联合作者(
Jeffrey E. Gerst Molecular Genetics, Weizmann Institute of Science, Israel, Israel,
1 protocol
联合评审(
Abhijit Kale Deciduous Therapeutics
10 protocols

Akira Karasawa New York Structural Biology Center
8 protocols

Alexandros C Kokotos Weill Cornell Medicine
18 protocols

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

编委
Gal Haimovich
  • Research scientist, Molecular Genetics, Weizmann Institute of Science, Israel
研究方向
  • 细胞生物学
  • mRNA localization, mRNA transfer, membrane nanotubes, exosomes
  • 已发表 protocol 2
课题组

教育背景

PhD, Technion - Israeli institute of technology, 2011

实验室信息

Jeffrey Gerst lab http://www.weizmann.ac.il/molgen/Gerst/
We are studying protein and mRNA localization in eukaryotic cells (mainly yeast & mammalian cells).
http://www.weizmann.ac.il/molgen/Gerst/

发表论文

  1. https://greenfluorescentblog.wordpress.com/about/list-of-publications/
  2. Haimovich, G., Zabezhinsky, D., Haas, B., Slobodin, B., Purushothaman, P., Fan, L., Levin, J. Z., Nusbaum, C. and Gerst, J. E. (2016). Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5' to 3' degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system". RNA 22(5): 660-666.
  3. Haimovich, G., Cohen-Zontag, O. and Gerst, J. E. (2016). A role for mRNA trafficking and localized translation in peroxisome biogenesis and function? Biochim Biophys Acta 1863(5): 911-921.
  4. Buxbaum, A. R.*, Haimovich, G.* and Singer, R. H. (2015). In the right place at the right time: visualizing and understanding mRNA localization. Nat Rev Mol Cell Biol 16(2): 95-109. (*Equal contribution)
  5. Somekh, J., Haimovich, G., Guterman, A., Dori, D. and Choder, M. (2014). Conceptual modeling of mRNA decay provokes new hypotheses. PLoS One 9(9): e107085.
  6. Haimovich, G., Medina, D. A., Causse, S. Z., Garber, M., Millan-Zambrano, G., Barkai, O., Chavez, S., Perez-Ortin, J. E., Darzacq, X. and Choder, M. (2013). Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis. Cell 153(5): 1000-1011.
  7. Haimovich, G., Choder, M., Singer, R. H. and Trcek, T. (2013). The fate of the messenger is pre-determined: a new model for regulation of gene expression. Biochim Biophys Acta 1829(6-7): 643-653.
  8. Harel-Sharvit, L., Eldad, N., Haimovich, G., Barkai, O., Duek, L. and Choder, M. (2010). RNA polymerase II subunits link transcription and mRNA decay to translation. Cell 143(4): 552-563.
  9. Goler-Baron, V., Selitrennik, M., Barkai, O., Haimovich, G., Lotan, R. and Choder, M. (2008). Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes. Genes Dev 22(15): 2022-2027.
  10. Lotan, R., Goler-Baron, V., Duek, L., Haimovich, G. and Choder, M. (2007). The Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanisms. J Cell Biol 178(7): 1133-1143.
  11. Kamer, I., Sarig, R., Zaltsman, Y., Niv, H., Oberkovitz, G., Regev, L., Haimovich, G., Lerenthal, Y., Marcellus, R. C. and Gross, A. (2005). Proapoptotic BID is an ATM effector in the DNA-damage response. Cell 122(4): 593-603.
  12. Weiss, M., Haimovich, G. and Pick, U. (2001). Phosphate and Sulfate uptake in the halotolerant alga Dunaliella are driven by Na+-symport mechanism. J Plant Physiology 158:1519-1525.



已发表 protocol 11篇
Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells
利用单分子荧光原位杂交进行贴壁动物细胞中RNA的检测

作者:Gal Haimovich and Jeffrey E. Gerst日期:11/05/2018,浏览量:24508,Q&A: 1
Transcription and RNA decay play critical roles in the process of gene expression and the ability to accurately measure cellular mRNA levels is essential for understanding this regulation. Here, we describe a single-molecule fluorescent in situ ...
Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)
芽殖酵母(酿酒酵母)质粒提取

作者:Gal Haimovich日期:07/20/2018,浏览量:7858,Q&A: 0
Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...
参审 protocol 4篇
Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
作者:Rachna Narayanan and Andrew C. Oates日期:03/20/2019,浏览量:10059,Q&A: 0
In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes ...
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Ectopic Gene Expression in Macrophages Using in vitro Transcribed mRNA
使用体外转录mRNA在巨噬细胞中进行基因异位表达
作者:Pallavi Chandra and Jennifer A. Philips日期:05/20/2018,浏览量:5043,Q&A: 1
Macrophages are immune cells that contribute to host defense through various mechanisms including phagocytosis and antigen presentation. Their antimicrobial capacity is subverted by clinically important intracellular pathogens such as Mycobacterium ...
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主审 protocol 97篇
Preparation of Drosophila Larval Blood Cells for Single-cell RNA Sequencing
果蝇幼虫血细胞单细胞RNA测序的制备
作者:Sudhir Gopal Tattikota and Norbert Perrimon日期:08/20/2021,浏览量:530,Q&A: 0

Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain

...
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CRISPR-mediated Labeling of Cells in Chick Embryos Based on Selectively Expressed Genes
基于选择性表达基因的CRISPR介导的鸡胚胎细胞标记
作者:Masahito Yamagata and Joshua R. Sanes日期:08/05/2021,浏览量:1242,Q&A: 0

The abilities to mark and manipulate specific cell types are essential for an increasing number of functional, structural, molecular, and developmental analyses in model organisms. In a few species, this can be accomplished by germline transgenesis;

...
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