• 卷册 11, 2021
  • 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3890

往期刊物(2021)

卷册: 11 期号: 2

生物化学

A mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases 用Mant-GDP分离实验比较小GTP蛋白的鸟嘌呤核苷酸的结合偏好 作者:Yuping Tan and Qingxiang Sun日期:01/20/2021,浏览量:2185,Q&A: 0

Small GTPases are cellular switches that are switched on when bound to GTP and switched off when bound to GDP. Different small GTPase proteins or those with mutations may bind to GTP or GDP with different relative affinities. However, small GTPases generally have very high affinities for guanine nucleotides, rendering it difficult to compare the

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Polyamine Transport Assay Using Reconstituted Yeast Membranes 利用重构酵母细胞膜的多胺转运实验 作者:Sarah van Veen, Shaun Martin, Marleen Schuermans and Peter Vangheluwe日期:01/20/2021,浏览量:1533,Q&A: 0

ATP13A2/PARK9 is a late endo-/lysosomal P5B transport ATPase that is associated with several neurodegenerative disorders. We recently characterized ATP13A2 as a lysosomal polyamine exporter, which sheds light on the molecular identity of the unknown mammalian polyamine transport system. Here, we describe step by step a protocol to measure

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Reverse Phase-high-performance Liquid Chromatography (RP-HPLC) Analysis of Globin Chains from Human Erythroid Cells 人类红细胞珠蛋白链的反相高效液相色谱(RP-HPLC)分析 作者:Anne Chalumeau, Giacomo Frati, Elisa Magrin and Annarita Miccio日期:01/20/2021,浏览量:1309,Q&A: 0

β-hemoglobinopathies are severe genetic disorders characterized either by the abnormal synthesis of the adult β-globin chains of the hemoglobin (Hb) tetramer (βS-globin chains) in sickle cell disease (SCD) or by the reduced β-globin production in β-thalassemia. The identification and quantification of globin chains are

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癌症生物学

Imaging of Human Cancer Cells in 3D Collagen Matrices 人类癌细胞的3D胶原基质成像 作者:Karin Pfisterer, Brooke Lumicisi and Maddy Parsons日期:01/20/2021,浏览量:2676,Q&A: 0

Research on cell migration and interactions with the extracellular matrix (ECM) was mostly focused on 2D surfaces in the past. Many recent studies have highlighted differences in migratory behaviour of cells on 2D surfaces compared to complex cell migration modes in 3D environments. When embedded in 3D matrices, cells constantly sense the

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Evaluation of Toxicity with Brine Shrimp Assay 通过卤虫实验进行毒性评价 作者:Christina N. Banti and Sotiris K. Hadjikakou日期:01/20/2021,浏览量:2602,Q&A: 0

The in vivo toxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) or their hydrogels such as with hydroxyethyl-methacrylate (HEMA) (pHEMA@SBAMs or pHEMA@CoMeDs) are evaluated by the brine shrimp assay. Thus individuals of Artemia salina larvae are incubated in saline solutions with

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免疫学

Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection System 利用基于T细胞活化报告基因的检测系统进行多重T细胞刺激测定 作者:Laurie G. Landry, Sarah E. Mann, Amanda M. Anderson and Maki Nakayama日期:01/20/2021,浏览量:1976,Q&A: 0

Immune tolerance and response are both largely driven by the interactions between the major histocompatibility complex (MHC) expressed by antigen presenting cells (APCs), T-cell receptors (TCRs) on T-cells, and their cognate antigens. Disordered interactions cause the pathogenesis of autoimmune diseases such as type 1 diabetes. Therefore, the

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Wounding Caenorhabditis elegans with Glass Wool 玻璃棉用于秀丽隐杆线虫的创伤研究 作者:Murugesan Pooranachithra, James Prabhanand Bhaskar and Krishnaswamy Balamurugan日期:01/20/2021,浏览量:1378,Q&A: 0

Research on wound healing majorly relies on rat, mice and other animal models. However, an alternative animal model ought to be brought in the field, pertaining to the stringent ethical issues owing to the use of animals in research. In this regard, Caenorhabdits elegans, a miniature model nematode gains the great attention of the researchers in

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微生物学

Bacterial Adhesion Kinetics in a High Throughput Setting in Seconds-minutes Time Resolution 高通量条件下细菌粘附动力学(Seconds-minutes时间分辨率) 作者:Nimrod Shteindel and Yoram Gerchman日期:01/20/2021,浏览量:1014,Q&A: 0
Bacterial surface adhesion, the first step in many important processes including biofilm formation and tissue invasion, is a fast process that occurs on a time scale of seconds. Adhesion patterns tend to be stochastic and spatially heterogeneous, especially when bacteria are present in low population densities and at early stages of adhesion to ...
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Determination of Antibacterial Activity of Film Coatings against Four Clinically Relevant Bacterial Strains 薄膜敷层对4种与临床相关菌株的抑菌活性测定 作者:Natalia A. Scilletta, Magdalena Pezzoni, Martín F. Desimone, Galo J. A. A. Soler-Illia, Martín G. Bellino and Paolo N. Catalano日期:01/20/2021,浏览量:1589,Q&A: 0

Antibacterial coatings have currently gained great importance in biomedical technology investigations. Because of the spatial arrangement of the film coatings, evaluation of antibacterial activity presents a new challenge regarding traditional bacterial counting methods. In this protocol, four clinically relevant pathogens, Salmonella typhimurium,

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Analysis of Pseudomonas aeruginosa c-di-GMP High and Low Subpopulations Using Flow-assisted Cell Sorting (FACS) and Quantitative Reverse Transcriptase PCR (qRT-PCR) 用荧光激活细胞分类术和荧光定量PCR技术分析铜绿假单胞菌环二鸟苷酸的高低亚群 作者:Catherine R. Armbruster and Matthew R. Parsek日期:01/20/2021,浏览量:1279,Q&A: 0

Cyclic diguanylate monophosphate (c-di-GMP) is a second messenger signaling molecule that drives the transition from planktonic to the biofilm mode of growth in many bacterial species. Pseudomonas aeruginosa has at least two surface sensing systems that produce c-di-GMP in response to surface attachment, the Wsp and Pil-Chp systems. We recently

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Bacterial Conjugation Protocol for Ruminant Mycoplasmas 反刍动物支原体细菌接合的实验方法 作者:Eveline Sagné, Christine Citti and Emilie Dordet-Frisoni日期:01/20/2021,浏览量:1007,Q&A: 0

In Mycoplasma agalactiae, two simultaneous processes of DNA transfer have been described that require direct cell-to-cell contact and are similar to conjugation. One involves the self-transmission of an integrative conjugative element (ICE) while the second concerns the horizontal transfer of large and small fragments of chromosomal DNA. Here, we

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A One-enzyme RT-qPCR Assay for SARS-CoV-2, and Procedures for Reagent Production SARS-CoV-2单酶RT-qPCR检测方法及试剂生产程序 作者:Sanchita Bhadra, Andre C. Maranhao, Inyup Paik and Andrew D. Ellington日期:01/20/2021,浏览量:1365,Q&A: 1

Given the scale of the ongoing COVID-19 pandemic, the need for reliable, scalable testing, and the likelihood of reagent shortages, especially in resource-poor settings, we have developed an RT-qPCR assay that relies on an alternative to conventional viral reverse transcriptases, a thermostable reverse transcriptase/DNA polymerase (RTX) (Ellefson

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神经科学

Characterization of Hippocampal Adult-borne Granule Cells in a Transient Cerebral Ischemia Model 海马成体新生颗粒细胞在短暂性脑缺血模型中的特征 作者:Mihai Ceanga, Madlen Guenther, Ina Ingrisch and Albrecht Kunze日期:01/20/2021,浏览量:907,Q&A: 0

Long-term consequences of stroke significantly impair the quality of life in a growing population of stroke survivors. Hippocampal adult neurogenesis has been hypothesized to play a role in the pathophysiology of cognitive and neuropsychiatric long-term sequelae of stroke. Reliable animal models of stroke are paramount to understanding their

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Low- and High-resolution Dynamic Analyses for Magnetic Resonance Spectroscopy Data 磁共振光谱数据的低高分辨率动态分析 作者:Reuben Rideaux日期:01/20/2021,浏览量:1486,Q&A: 0

Magnetic resonance spectroscopy (MRS) can be used to measure in vivo concentrations of neurometabolites. This information can be used to identify neurotransmitter involvement in healthy (e.g., perceptual and cognitive processes) and unhealthy brain function (e.g., neurological and psychiatric illnesses). The standard approach for analyzing MRS

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Preparation of Synaptoneurosomes to Study the Synapse in the Murine Cerebral Cortex 用于研究小鼠大脑皮层突触的突触神经小体的制备 作者:Ariel Diaz, Enrique Torre and Manuel Yepes日期:01/20/2021,浏览量:1515,Q&A: 1

The synapse is a complex structure where the transmission of information takes place. Synaptic dysfunction is one of the earliest pathophysiological events in several diseases, such as traumatic brain injury, cerebral ischemia, and neurodegenerative diseases. Thus, a methodology to study synaptic structure and function is crucial for the

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植物科学

Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts 纤维素酶和离析酶-PEG介导的苔藓原生质体转化 作者:Rituraj Batth, Ilenuta Simina Cuciurean, Satish Kumar Kodiripaka, Sarah Skovlund Rothman, Caroline Greisen and Henrik Toft Simonsen日期:01/20/2021,浏览量:1643,Q&A: 0
This protocol describes the generation of protoplasts from protonemal tissue of the moss Physcomitrium patens (syn. Physcomitrella patens), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) mediated transformation. The protonemal tissue grown in liquid suspension was harvested and treated with ...
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Design and Construction of Repacked Soil Columns for Measuring Solute Transport, Plant Growth and Soil Biota 用于测量溶质迁移、植物生长和土壤生物区系的重装土柱的设计和建造 作者:Israel Ikoyi and Achim Schmalenberger日期:01/20/2021,浏览量:1231,Q&A: 0

Researchers face a number of challenges in the construction of soil columns which can affect the outcome of their experiments. The use of intact soil cores closely mimics actual field conditions. However, the excavation of large intact soil cores is a time-consuming, labor-intensive process and may lead to soil compaction that would influence the

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干细胞

Histological and Immunohistochemical Examination of Stem Cell Proliferation and Reepithelialization in the Wounded Skin 损伤皮肤干细胞增殖和再生的组织学和免疫组化检测 作者:Rupali Gund, Ravindra Zirmire, Haarshaadri J., Gaurav Kansagara and Colin Jamora日期:01/20/2021,浏览量:2617,Q&A: 0

The skin is the largest organ that protects our body from the external environment and it is constantly exposed to pathogenic insults and injury. Repair of damage to this organ is carried out by a complex process involving three overlapping phases of inflammation, proliferation and remodeling. Histological analysis of wounded skin is a convenient

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