• 卷册 11, 2021
  • 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3947

往期刊物(2021)

卷册: 11 期号: 5

生物化学

Simultaneous Imaging of Single Protein Size, Charge, and Binding Using A Protein Oscillation Approach 利用蛋白质振荡方法同时成像单个蛋白质的大小、电荷和结合状态 作者:Guangzhong Ma, Zijian Wan and Shaopeng Wang日期:03/05/2021,浏览量:1235,Q&A: 0

Electrophoresis and Western blot are important tools in protein research for detection and identification of proteins. These traditional techniques separate the proteins based on size and charge differences and identify the proteins by antibody binding. Over the past decade, the emergence of single-molecule techniques has shown great potential in

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Identification of Intrinsic RNA Binding Specificity of Purified Proteins by in vitro RNA Immunoprecipitation (vitRIP) 体外RNA免疫沉淀(vitRIP)鉴定纯化蛋白的RNA结合特异性 作者:Marisa Müller, Tamas Schauer and Peter B. Becker日期:03/05/2021,浏览量:941,Q&A: 0

RNA-protein interactions are often mediated by dedicated canonical RNA binding domains. However, interactions through non-canonical domains with unknown specificity are increasingly observed, raising the question how RNA targets are recognized. Knowledge of the intrinsic RNA binding specificity contributes to the understanding of target

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生物物理学

Automated Analysis of Cerebrospinal Fluid Flow and Motile Cilia Properties in The Central Canal of Zebrafish Embryos 斑马鱼胚胎中央管中脑脊液流动和运动纤毛特性的自动分析 作者:Olivier Thouvenin, Yasmine Cantaut-Belarif, Ludovic Keiser, François Gallaire and Claire Wyart日期:03/05/2021,浏览量:773,Q&A: 0

Circulation of cerebrospinal fluid (CSF) plays an important role during development. In zebrafish embryo, the flow of CSF has been found to be bidirectional in the central canal of the spinal cord. In order to compare conditions and genetic mutants across each other, we recently automated the quantification of the velocity profile of exogenous

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Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy 用原子力显微镜描述DNA解旋酶PriA与停止的DNA复制叉的相互作用 作者:Yaqing Wang, Zhiqiang Sun, Piero R. Bianco and Yuri L. Lyubchenko日期:03/05/2021,浏览量:943,Q&A: 0

In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. ssDNA-binding protein (SSB) is typically present at the abandoned forks,

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癌症生物学

Strategy of Isolating ‘Primed’ Tumor Initiating Cells Based on Mitochondrial Transmembrane Potential 基于线粒体跨膜电位分离“启动”肿瘤起始细胞的策略 作者:Brian Spurlock, Vidya Sagar Hanumanthu and Kasturi Mitra日期:03/05/2021,浏览量:1214,Q&A: 0

Various stem cells have been found to be dependent on mitochondrial energetics. The role of mitochondria in regulating the self-renewal of normal stem cells and stem-like tumor initiating cells (TICs) is increasingly being appreciated. We proposed that TIC populations have a sub population of cells that are “primed” by mitochondria for

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细胞生物学

Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells 用RUSH蛋白分选法追踪HeLa细胞中的蛋白囊泡 作者:Natalia Pacheco-Fernandez, Mehrshad Pakdel and Julia von Blume日期:03/05/2021,浏览量:1055,Q&A: 0

Monitoring vesicle trafficking is an excellent tool for the evaluation of protein dynamics in living cells. Such study is key for the understanding of protein sorting and secretion. Recent developments in microscopy, as well as new methodologies developed to study synchronized trafficking of proteins, allowed a better understanding of signaling,

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Live Intravital Intestine with Blood Flow Visualization in Neonatal Mice Using Two-photon Laser Scanning Microscopy 用双光子激光扫描显微镜观察新生小鼠活体肠内血流 作者:Yuhki Koike, Bo Li, Yong Chen, Niloofar Ganji, Mashriq Alganabi, Hiromu Miyake, Carol Lee, Alison Hock, Richard Wu, Keiichi Uchida, Mikihiro Inoue, Paul Delgado-Olguin and Agostino Pierro日期:03/05/2021,浏览量:951,Q&A: 0

This protocol describes a novel technique to investigate the microcirculation dynamics underlying the pathology in the small intestine of neonatal mice using two-photon laser-scanning microscopy (TPLSM). Recent technological advances in multi-photon microscopy allow intravital analysis of different organs such as the liver, brain and intestine.

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微生物学

Assay for Assessing Mucin Binding to Bacteria and Bacterial Proteins 评估粘蛋白与细菌和细菌蛋白结合的实验 作者:Lubov S. Grigoryeva, Saima Rehman, Richard C. White, James A. Garnett and Nicholas P. Cianciotto日期:03/05/2021,浏览量:885,Q&A: 0

Legionella pneumophila, a Gram-negative bacterium and the causative agent of Legionnaires’ disease, exports over 300 effector proteins/virulence factors, through its type II (T2SS) and type IV secretion systems (T4SS). One such T2SS virulence factor, ChiA, not only functions as a chitinase, but also as a novel mucinase, which we believe aids

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Primer ID Next-Generation Sequencing for the Analysis of a Broad Spectrum Antiviral Induced Transition Mutations and Errors Rates in a Coronavirus Genome 用于分析冠状病毒基因组中广谱抗病毒药物诱导的过渡突变和错误率的引物ID下一代测序 作者:Shuntai Zhou, Collin S. Hill, Michael U. Clark, Timothy P. Sheahan, Ralph Baric and Ronald Swanstrom日期:03/05/2021,浏览量:1074,Q&A: 0

Next generations sequencing (NGS) has become an important tool in biomedical research. The Primer ID approach combined with the MiSeq platform overcomes the limitation of PCR errors and reveals the true sampling depth of population sequencing, making it an ideal tool to study mutagenic effects of potential broad-spectrum antivirals on RNA viruses.

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Candida Biofilm Formation Assay on Essential Oil Coated Silicone Rubber 精油包覆硅橡胶上念珠菌生物膜形成的实验研究 作者:Gulcan Sahal, Herman J. Woerdenbag, Wouter L. J. Hinrichs, Anita Visser, Henny C. van der Mei and Isil Seyis Bilkay日期:03/05/2021,浏览量:645,Q&A: 0

Development of biofilm associated candidemia for patients with implanted biomaterials causes an urgency to develop antimicrobial and biofilm inhibitive coatings in the management of recalcitrant Candida infections. Recently, there is an increase in the number of patients with biofilm formation and resistance to antifungal therapy. Therefore, there

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Generation and Implementation of Reporter BHK-21 Cells for Live Imaging of Flavivirus Infection 黄病毒感染活体显像报告细胞BHK-21的制备与实施 作者:Jorge L. Arias-Arias and Rodrigo Mora-Rodríguez日期:03/05/2021,浏览量:469,Q&A: 0

The genus Flavivirus within the family Flaviviridae includes many viral species of medical importance, such as yellow fever virus (YFV), Zika virus (ZIKV), and dengue virus (DENV), among others. Presently, the identification of flavivirus-infected cells is based on either the immunolabeling of viral proteins, the application of recombinant

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分子生物学

Trypanosomatid, fluorescence-based in vitro U-insertion/U-deletion RNA-editing (FIDE) 基于荧光的锥虫体体外尿嘧啶插入或删除RNA编辑(FIDE) 作者:Wolf-Matthias Leeder, Elisabeth Kruse and H. Ulrich Göringer日期:03/05/2021,浏览量:422,Q&A: 0

Gene expression within the mitochondria of African trypanosomes and other protozoan organisms relies on a nucleotide-specific RNA-editing reaction. In the process exclusively uridine (U)-nucleotides are site-specifically inserted into and deleted from sequence-deficient primary transcripts to convert them into translatable mRNAs. The reaction is

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Defined Mutant Library Sequencing (DML-Seq) for Identification of Conditional Essential Genes 确定突变体文库测序(DML-Seq)用于鉴定的条件必需基因 作者:Shuai Shao, Lifan Wei, Feng Xia, Yuanxing Zhang and Qiyao Wang日期:03/05/2021,浏览量:437,Q&A: 0

Transposon insertion sequencing (TIS) is an emerging technique which utilizes a massive transposon mutant library to screen specific phenotype and determine the conditional essential genetic requirements for bacterial fitness under distinct conditions combined with high-throughput parallel sequencing technology. Compared with a massive mutant

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神经科学

Generation of Mouse Primary Hypothalamic Neuronal Cultures for Circadian Bioluminescence Assays 培养小鼠原发性下丘脑神经元用于昼夜生物发光分析 作者:Cosima X. Schmidt, Anthony H. Tsang and Henrik Oster日期:03/05/2021,浏览量:446,Q&A: 0

An endogenous circadian clock system enables organisms to adapt to time-of-day dependent environmental changes. In consequence, most physiological processes exhibit daily rhythms of, e.g., energy metabolism, immune function, sleep, or hormone production. Hypothalamic circadian clocks have been identified to play a particular role in coordinating

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Dissociating Behavior and Spatial Working Memory Demands Using an H Maze 利用H迷宫研究分离行为和空间工作记忆需求 作者:Hannah S. Wirtshafter, Moqing Quan and Matthew A. Wilson日期:03/05/2021,浏览量:648,Q&A: 0

The development of mazes for animal experiments has allowed for the investigation of cognitive maps and place cells, spatial working memory, naturalistic navigation, perseverance, exploration, and choice and motivated behavior. However, many mazes, such as the T maze, currently developed to test learning and memory, do not distinguish temporally

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Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation 小鼠心脏灌流、脑组织解剖与固定 作者:Jinyun Wu, Yuqi Cai, Xiaoyang Wu, Yue Ying, Yilin Tai and Miao He日期:03/05/2021,浏览量:829,Q&A: 0
Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to preserve tissue and cell structure. Here we provide a step by step guide for ...
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植物科学

Chromatographic Analysis for Targeted Metabolomics of Antioxidant and Flavor-Related Metabolites in Tomato 番茄抗氧化剂和风味相关代谢产物靶向代谢组学的色谱分析 作者:Nergiz Gürbüz Çolak, Neslihan Tek Eken, Anne Frary and Sami Doğanlar日期:03/05/2021,浏览量:780,Q&A: 0

Targeted metabolomics is a useful approach to evaluate crop breeding studies. Antioxidant and flavor-related traits are of increasing interest and are considered quality traits in tomato breeding. The present study presents chromatographic methods to study antioxidants (carotenoids, vitamin C, vitamin E, phenolic compounds, and glutathione) and

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干细胞

Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders 人来源iPSC获得的神经祖细胞(NPCs)的产生作为神经和线粒体疾病的药物发现模型 作者:Annika Zink, Pawel Lisowski and Alessandro Prigione日期:03/05/2021,浏览量:794,Q&A: 0

The high attrition rate in drug development processes calls for additional human-based model systems. However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced pluripotent stem cells (iPSCs) has revolutionized the field of neuronal disease modeling and drug discovery.

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