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生物化学

Binding to Secreted Bone Matrix in vitro 分泌骨基质结合的体外试验 作者:Aurélie Jeanne Tormo, Christian Beauséjour and Jean-François Gauchat日期:02/20/2014,浏览量:7878,Q&A: 0
This method examines the bone matrix binding capacity of proteins. Using osteogenic differentiation medium, multipotent stromal cells (MSC) are induced to differentiate into osteocytes in vitro and to secrete bone matrix. The latter is confirmed using Alizarin red S staining, which detects the presence of calcific deposits ...
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A Surface Plasmon Resonance Method to Study HCV NS5B Inhibitors 采用表面等离子体共振研究HCV NS5B抑制因子 作者:Melanie Wong and Giuseppe A. Papalia日期:02/20/2014,浏览量:7794,Q&A: 0
Surface Plasmon Resonance (SPR) technology is a well-established platform used to evaluate the kinetic parameters of protein-small molecule interactions. Below, we describe the use of the ProteOn XPR36 biosensor from Bio-Rad (Hercules, CA) to evaluate the binding of small molecule inhibitors to recombinant NS5B protein. The high pI (> 9) of ...
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癌症生物学

Isolation of Mammary Epithelial Cells and Fibroblasts From Mouse Tumor 小鼠肿瘤乳腺上皮细胞与成纤维细胞的分离 作者:Shiva Kazerounian日期:02/20/2014,浏览量:18001,Q&A: 0
Developing cancer therapeutics requires the ability to investigate their effects using in vitro models of a specific type of tumor. This protocol provides a method for the isolation and adoption to growth in culture of cells from primary tumors. This is particularly valuable for studying mouse models where original tumor cells can be ...
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In vivo Extravasation Assay 癌细胞外渗的体内试验 作者:Elisa Penna and Daniela Taverna日期:02/20/2014,浏览量:12338,Q&A: 1
Tumor metastases develop when disseminated intravascular cancer cells acquire the ability to arrest by adhering to the capillary walls of distant organs, actively extravasate into their parenchyma, proliferate and establish secondary colonies. The extravasation assay described here is an in vivo technique aimed to analyze the ability of ...
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细胞生物学

Lysosomal Amino Acid Efflux Assay 溶酶体氨基酸外流检测试验 作者:Chunlei Cang and Dejian Ren日期:02/20/2014,浏览量:8452,Q&A: 0
As the cellular “recycling” organelle, lysosomes break down proteins into amino acids, which are then transported into cytosol for reuse by various amino acid transporters. The transport rate of an amino acid is presumably regulated by cellular conditions such as organelle pH, membrane potential and metabolic states. Because of their ...
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免疫学

Isolation of Multipotent Stromal Cells from Mouse Bone Marrow 从小鼠骨髓中分离多能间质干细胞 作者:Aurélie Jeanne Tormo, Moutih Rafei and Jean-François Gauchat日期:02/20/2014,浏览量:8236,Q&A: 0
Generating mouse multipotent stromal cells (MSC) from bone-marrow cells is usefull for a wide range of applications. Effectively, these MSC can differentiate into adipocytes, osteocytes [See “Binding to Secreted Bone Matrix in vitro” (Tormo et al., 2014)] or ...
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DNA PCR Assays for Igh Rearrangement 基于DNA的PCR法分析免疫球蛋白重链(Igh)的重排 作者:Yusuke Satoh, Takao Sudo and Takafumi Yokota日期:02/20/2014,浏览量:8645,Q&A: 0
This protocol is used for the detection of immunoglobulin heavy (H) chain rearrangements. This PCR-based assay enables detection of DH-JH recombination in cultured hematopoietic cells (Schlissel et al., 1991; Satoh et al., 2013) [e.g. ES-derived cells (Satoh et al., 2013)].
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Chikungunya ELISA Protocol 酶联免疫吸附法(ELISA)测定基孔肯雅病毒 作者:Caitlin Mattos Briggs, Amanda Piper and Raquel Hernandez日期:02/20/2014,浏览量:8529,Q&A: 0
This protocol will result in the accurate qualitative measurement of anti-Chikungunya virus antibody (Ab) from infected mouse tissue or serum. This assay was developed by Dr. Caitlin Briggs, Arbovax, Inc. Chikungunya is a BL3 agent and should be handled in a biosafety level 3 laboratory under BL3 conditions. This protocol was used in the ...
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Isolation and Culture of Peritoneal Cell-derived Mast Cells 来源于腹水的肥大细胞分离和培养 作者:Krisztina V. Vukman, Martin Metz, Marcus Maurer and Sandra M. O’Neill日期:02/20/2014,浏览量:13720,Q&A: 2
The generation of mast cells for in vitro studies comes from a variety of sources including mast cell lines (MC/9) (McCurdy et al., 2001), bone marrow-derived mast cells (BMMCs) (Supajatura et al., 2001), skin-derived mast cells (FSMCs) (Matsushima et al., 2004), peritoneal-derived mast cells (PMCs) (Hochdorfer ...
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Isolation and Culture of Bone Marrow-derived Mast Cells 骨髓源肥大细胞的分离和培养 作者:Krisztina V. Vukman, Martin Metz, Marcus Maurer and Sandra M. O’Neill日期:02/20/2014,浏览量:18889,Q&A: 5
The generation of mast cells for in vitro studies comes from a variety of sources including mast cell lines (MC/9) (McCurdy et al., 2001), bone marrow-derived mast cells (BMMCs) (Supajatura et al., 2001), skin-derived mast cells (FSMCs) (Matsushima et al., 2004), peritoneal-derived mast cells (PMCs) (Hochdorfer ...
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微生物学

An ex vivo Model of HIV-1 Infection in Human Lymphoid Tissue and Cervico-vaginal Tissue HIV-1感染人淋巴组织和子宫颈阴道组织的体外模型 作者:Andrea Introini, Christophe Vanpouille, Jean Charles Grivel and Leonid Margolis日期:02/20/2014,浏览量:13131,Q&A: 0
Human tissue explants are a valuable tool to study the interactions between host and infectious agents. They reliably mimic many important aspects of tissue cytoarchitecture and functions and allow us the investigation of the mechanisms of microbial pathogenesis under controlled laboratory conditions. One of the advantages of this system is that, ...
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干细胞

Limiting Dilution Assays to Determine Frequencies of Lymphohematopoietic Progenitors 有限稀释法测定淋巴造血祖细胞的比例 作者:Takafumi Yokota and Yusuke Satoh日期:02/20/2014,浏览量:8630,Q&A: 0
This protocol is useful to determine the frequencies of lymphohematopoietic progenitors in tested samples. To effectively support the growth and differentiation of primitive lymphohematopoietic progenitors, complex signals from stromal cells are important. Several stromal cell lines are known to support both lymphoid and myeloid cells ...
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