细胞生物学

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    现刊
    CRISPR/Cas9 Gene Editing of HeLa Cells to Tag Proteins with mNeonGreen
    mNeonGreen对 HeLa 细胞进行 CRISPR/Cas9 基因编辑以标记蛋白质
    作者:Sachin Surve and Alexander Sorkin日期:05/20/2022,浏览量:1002,Q&A: 0
    [Abstract]

    Subcellular localization dynamics of proteins involved in signal transduction processes is crucial in determining the signaling outcome. However, there is very limited information about the localization of endogenous signaling proteins in living cells. For example, biochemical mechanisms underlying the signaling pathway from epidermal growth

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    Measurement of Cell Intrinsic TGF-β Activation Mediated by the Integrin αvβ8
    整合素αvβ8介导的细胞固有TGF-β活化测定
    作者:Robert Ian Seed and Stephen Lloyd Nishimura日期:04/20/2022,浏览量:1201,Q&A: 0
    [Abstract]

    Transforming growth factor beta (TGF-β) is a multi-functional cytokine that plays a significant role in multiple diseases, including fibrosis and tumor progression. Whilst the biologic effects of TGF-β are well characterized, it is unclear how TGF-β signaling is regulated to impart specific responses within certain cell types. One

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    Activation of Mitochondrial Ca2+ Oscillation and Mitophagy Induction by Femtosecond Laser Photostimulation
    飞秒激光光刺激激活线粒体 Ca2+ 振荡和线粒体自噬诱导
    作者:Xiaoying Tian and Hao He日期:04/05/2022,浏览量:418,Q&A: 0
    [Abstract]

    Ultra-precise stimulation solely to individual mitochondria, without any influence to the whole cell, is quite difficult by traditional biochemical reagents. In mitophagy research, the mitochondria and even the whole cell usually suffer irreversible and great damage caused by treatment with potent chemicals. In this protocol, we present the

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    A Hypersensitive Genetically Encoded Fluorescent Indicator (roGFP2-Prx1) Enables Continuous Measurement of Intracellular H2O2 during Cell Micro-cultivation
    超灵敏基因编码荧光指示剂 (roGFP2-Prx1) 在细胞微培养期间连续测量细胞内 H2O2
    作者:Veronica Gast, Verena Siewers and Mikael Molin日期:02/05/2022,浏览量:1268,Q&A: 0
    [Abstract]

    Hydrogen peroxide (H2O2) is a toxic oxidant produced as a byproduct of several biological processes. At too high levels of hydrogen peroxide cells will experience oxidative stress, leading to a cellular response to decrease its levels and to protect the cells. Previously, methods used to study and quantify intracellular H2O2 have been limited by

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    Chronic Daily House Dust Mite Exposure in Mice is an Effective Model to Quantify the Effect of Pharmacologic Agents on Discrete Stages of Artery Remodeling in Pulmonary Hypertension
    小鼠每日慢性房尘螨暴露是一种量化药物对肺动脉高压动脉重塑离散阶段影响的有效模型
    作者:Lea C. Steffes and Maya E. Kumar日期:01/05/2022,浏览量:1477,Q&A: 0
    [Abstract]

    Pulmonary hypertension (PH) is a heterogenous and incurable disease marked by varying degrees of pulmonary vascular remodeling. This vascular remodeling, which includes thickening of the smooth muscle layer (an early finding) and formation of occlusive neointimal lesions (a late finding) in the pulmonary arteries, is a major driver of morbidity

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    In vitro Fluid Shear Stress Induced Sclerostin Degradation and CaMKII Activation in Osteocytes
    体外流体剪切应力诱导骨细胞中的硬化蛋白降解和 CaMKII 激活
    [Abstract]

    Bone is a dynamic tissue that adapts to changes in its mechanical environment. Mechanical stimuli pressurize interstitial fluid in the lacunar-canalicular system within the bone matrix, causing fluid shear stress (FSS) across bone embedded, mechano-sensitive osteocytes. Therefore, modeling this mechanical stimulus in vitro is vital for identifying

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    Modeling the Nonlinear Dynamics of Intracellular Signaling Networks
    细胞内信号网络的非线性动力学建模
    作者:Oleksii S. Rukhlenko and Boris N. Kholodenko日期:07/20/2021,浏览量:2159,Q&A: 0
    [Abstract]

    This protocol illustrates a pipeline for modeling the nonlinear behavior of intracellular signaling pathways. At fixed spatial points, nonlinear signaling dynamics are described by ordinary differential equations (ODEs). At constant parameters, these ODEs may have multiple attractors, such as multiple steady states or limit cycles. Standard

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    Ethanol-induced Sedative Behavior: An Assay to Investigate Increased Dopamine Signaling in Caenorhabditis elegans
    乙醇诱导的镇静行为:一项在秀丽隐杆线虫研究增加多巴胺信号
    作者:Anuradha Singh, Kavita Babu and Pratima Pandey日期:07/05/2021,浏览量:1761,Q&A: 0
    [Abstract]

    Dopamine (DA) signaling affects locomotion, feeding, learning, and memory in C. elegans. Various assays have been developed to study the proteins involved in these behaviors; however, these assays show behavioral output only when there is a drastic change in DA levels. We designed an assay capable of observing behavioral output even with only

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    AIMTOR, a BRET Biosensor for Live Recording of mTOR Activity in Cell Populations and Single Cells
    一种用于实时记录细胞群和单个细胞中mTOR活性的BRET生物传感器AIMTOR
    作者:Nathalie Bouquier, Enora Moutin, Julie Perroy and Vincent Ollendorff日期:04/20/2021,浏览量:2393,Q&A: 0
    [Abstract]

    Mammalian target of rapamycin (mTOR) controls many crucial cellular functions, including protein synthesis, cell size, energy metabolism, lysosome and mitochondria biogenesis, and autophagy. Consequently, deregulation of mTOR signaling plays a role in numerous pathological conditions such as cancer, metabolic disorders and neurological diseases.

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    A Versatile Protocol to Quantify BCR-mediated Phosphorylation in Human and Murine B Cell Subpopulations
    量化人类和小鼠B细胞亚群中BCR介导的磷酸化的通用实验方法
    作者:Jasper Rip, Rudi W. Hendriks and Odilia B. J. Corneth日期:02/05/2021,浏览量:1800,Q&A: 0
    [Abstract]

    Signal transduction is the process by which molecular signals are transmitted from the cell surface to its interior, resulting in functional changes inside the cell. B cell receptor (BCR) signaling is of crucial importance for B cells, as it regulates their differentiation, selection, survival, cellular activation and proliferation. Upon BCR

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