癌症生物学

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    现刊
    Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer
    多色流式细胞术综合分析乳腺癌小鼠模型中肿瘤免疫渗透
    作者:Ana S. Almeida, Miriam R. Fein and Mikala Egeblad日期:06/05/2021,浏览量:3106,Q&A: 0
    [Abstract]

    Flow cytometry is a popular laser-based technology that allows the phenotypic and functional characterization of individual cells in a high-throughput manner. Here, we describe a detailed procedure for preparing a single-cell suspension from mammary tumors of the mouse mammary tumor virus-polyoma middle T (MMTV-PyMT) and analyzing these cells by

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    In-vivo gp100-specific Cytotoxic CD8+ T Cell Killing Assay
    体内gp100特异的细胞毒CD8+ T 细胞杀伤实验
    作者:Mahdia Benkhoucha, Nicolas Molnarfi, Elodie Belnoue, Madiha Derouazi and Patrice H Lalive日期:11/20/2018,浏览量:6307,Q&A: 0
    [Abstract] Cytotoxic CD8+ T lymphocytes (CTLs) represent a crucial component of the adaptive immune system and play a prominent role in the anti-tumor immune responses of both mice and humans.

    Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived ...
    Glioma Induction by Intracerebral Retrovirus Injection
    脑内注射逆转录病毒诱导胶质瘤
    作者:Ravinder K Verma , Fanghui Lu and Qing Richard Lu日期:07/20/2017,浏览量:8411,Q&A: 0
    [Abstract] Glioblastoma (GBM) is the most common primary brain cancer in adults and has a poor prognosis. It is characterized by a high degree of cellular infiltration that leads to tumor recurrence, atypical hyperplasia, necrosis, and angiogenesis. Despite aggressive treatment modalities, current therapies are ineffective for GBM. Mouse GBM models not only ...
    Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model
    采用联体模型在PyMT乳腺肿瘤模型中测定白血球起源
    作者:Ruth A. Franklin and Ming O. Li日期:08/20/2015,浏览量:9001,Q&A: 0
    [Abstract] Tumors develop in a complex microenvironment alongside numerous cell types that impact their survival. Immune cells make up a large proportion of these accessory cells and many are known to promote tumor progression. Macrophages, in particular, are associated with poor patient prognosis and are therefore potential candidates for therapeutic ...
    Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model
    前列腺癌模型中骨髓来源的抑制性细胞和T淋巴细胞的过继转移
    作者:Libo Yan and Yan Xu日期:08/20/2015,浏览量:8156,Q&A: 0
    [Abstract] The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP mouse tumor. TRAMP-C2 is tumorigenic when ...
    Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)
    肿瘤相关巨噬细胞(TAM)和肿瘤相关树突细胞(TADC)的纯化
    作者:Damya Laoui, Eva Van Overmeire, Jiri Keirsse, Kiavash Movahedi and Jo A Van Ginderachter日期:11/20/2014,浏览量:25678,Q&A: 1
    [Abstract] Tumors are heterogeneous microenvironments where complex interactions take place between neoplastic cells and infiltrating inflammatory cells, such as tumor-associated macrophages (TAM) and tumor-associated dendritic cells (TADC). The relevance of tumor-infiltrating mononuclear myeloid cells is underscored by clinical studies showing a correlation ...
    Natural Killer Cell Transfer Assay
    自然杀伤细胞迁移试验
    作者:Lee-Hwa Tai, Christiano Tanese de Souza, Andrew P. Makrigiannis and Rebecca Ann C. Auer日期:08/20/2013,浏览量:11206,Q&A: 0
    [Abstract] Natural Killer (NK) cells are cytotoxic lymphocytes that constitute a major component of the innate immune system. Immunosurveillance of the host by NK cells for malignant and virally-infected cells results in direct cytotoxicity and the production of cytokines to enhance the immune response. This protocol will describe the adoptive transfer of ...
    Lung Clearance Assay
    肺部清除试验
    作者:Pinchas Tsukerman and Ofer Mandelboim日期:03/05/2013,浏览量:9675,Q&A: 0
    [Abstract] Lung clearance assay tests the ability of innate immune cells (mainly NK cells) to eradicate in vivo cells injected via the tail vein of the mice. This assay helps to elucidate the role played by NK cells and their receptors (if the mice are genetically modified) against various human and mouse targets in an in vivo setting ...