细胞生物学

分类

    现刊
    Electron Tomography to Study the Three-dimensional Structure of the Reovirus Egress Pathway in Mammalian Cells
    电子断层扫描研究哺乳动物细胞呼肠孤病毒出口途径的三维结构
    作者:Isabel Fernández de Castro, Jose Jesús Fernández, Terence S. Dermody and Cristina Risco日期:07/05/2021,浏览量:1501,Q&A: 0
    [Abstract]

    Mammalian orthoreoviruses (reoviruses) are nonenveloped, double-stranded RNA viruses that replicate and assemble in cytoplasmic membranous organelles called viral inclusions (VIs). To define the cellular compartments involved in nonlytic reovirus egress, we imaged viral egress in infected, nonpolarized human brain microvascular endothelial cells

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    Rice Root Hair Phenotypes Imaged by Cryo-SEM
    水稻根毛表型的低温扫描电镜成像
    [Abstract]

    Cryo-scanning electron microscopy (cryo-SEM) was first introduced for scientific use in the 1980s. Since then, cryo-SEM has become a routine technique for studying the surfaces and internal structures of biological samples with a high water content. In contrast to traditional SEM, cryo-SEM requires no sample pretreatment processes; thus, we can

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    Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy
    单粒子低温电子显微镜双态微管馏分的制备
    作者:Corbin Black, Daniel Chen Dai, Katya Peri, Muneyoshi Ichikawa and Khanh Huy Bui日期:06/05/2021,浏览量:1852,Q&A: 0
    [Abstract]

    Over the years, studying the ultrastructure of the eukaryotic cilia/flagella using electron microscopy (EM) has contributed significantly toward our understanding of ciliary function. Major complexes in the cilia, such as inner and outer dynein arms, radial spokes, and dynein regulatory complexes, were originally discovered by EM. Classical

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    Automated Analysis of Cell Surface Ruffling: Ruffle Quantification Macro
    细胞表面Ruffling的自动分析:Ruffle的宏观量化
    作者:Nicholas D. Condon, Jennifer L. Stow and Adam A. Wall日期:01/20/2020,浏览量:2673,Q&A: 1
    [Abstract] Cell surface protrusions include F-actin rich, wave-like ruffles that are erected transiently in response to stimuli and during cell migration. Macrophages are innate immune cells that ruffle constitutively and more dramatically in cells activated by pathogens. Dorsal ruffles and their resulting macropinosomes are key sites for environmental ...
    High Throughput Traction Force Microscopy for Multicellular Islands on Combinatorial Microarrays
    组合微阵列多细胞岛的高通量牵引力显微镜实验
    作者:Ian C. Berg and Gregory H. Underhill日期:11/05/2019,浏览量:3747,Q&A: 0
    [Abstract] The composition and mechanical properties of the cellular microenvironment along with the resulting distribution of cellular devolved forces can affect cellular function and behavior. Traction Force Microscopy (TFM) provides a method to measure the forces applied to a surface by adherent cells. Numerous TFM systems have been described in ...
    Cell Wall Compositional Analysis of Rice Culms
    水稻茎杆的细胞壁组成分析
    作者:Lanjun Zhang, Baocai Zhang and Yihua Zhou日期:10/20/2019,浏览量:2477,Q&A: 0
    [Abstract] The plant cell wall is a complicated network that is mainly constituted of polysaccharides, such as cellulose, hemicellulose and pectin. Many noncellulosic polysaccharides are further acetylated, which confers these polymers flexible physicochemical properties. Due to the significance of cell wall in plant growth and development, the analytic ...
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:3327,Q&A: 0
    [Abstract] All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...
    An Image Analysis Pipeline to Quantify Emerging Cracks in Materials or Adhesion Defects in Living Tissues
    分析活体组织中出现的材料裂缝或附着力缺陷的图像分析法
    作者:Stéphane Verger, Guillaume Cerutti and Olivier Hamant日期:10/05/2018,浏览量:4283,Q&A: 0
    [Abstract] Microcracks in materials reflect their mechanical properties. The quantification of the number or orientation of such cracks is thus essential in many fields, including engineering and geology. In biology, cracks in soft tissues can reflect adhesion defects, and the analysis of their pattern can help to deduce the magnitude and orientation of ...
    Sleeping Beauty Transposon-based System for Rapid Generation of HBV-replicating Stable Cell Lines
    使用基于睡美人转座子的系统快速生成HBV复制稳定细胞系
    作者:Jin-Wei Zheng, Jia-Li Cao and Quan Yuan 日期:07/05/2018,浏览量:6237,Q&A: 0
    [Abstract] The stable HBV-transfected cell lines, which based on stable integration of replication-competent HBV genome into hepatic cells, are widely used in basic research and antiviral drug evaluation against HBV. However, previous reported strategies to generate HBV-replicating cell lines, which primarily rely on random integration of exogenous DNA by ...
    Transmission Electron Microscopy for Analysis of Mitochondria in Mouse Skeletal Muscle
    使用透射电子显微镜技术分析小鼠骨骼肌线粒体
    作者:Joseph D. McMillan and Michael A. Eisenback日期:05/20/2018,浏览量:13643,Q&A: 0
    [Abstract] Skeletal muscle is the most abundant tissue in the human body and regulates a variety of functions including locomotion and whole-body metabolism. Skeletal muscle has a plethora of mitochondria, the organelles that are essential for aerobic generation of ATP which provides the chemical energy to fuel vital functions such as contraction. The number ...