生物化学

分类

    现刊
    Quantitative Characterization of the Amount and Length of (1,3)-β-D-glucan for Functional and Mechanistic Analysis of Fungal (1,3)-β-D-glucan Synthase
    通过(1,3)-β-d-葡聚糖量和长度的定量表征对真菌(1,3)-β-d-葡聚糖合酶进行功能和机理分析
    作者:Abhishek Chhetri, Anna Loksztejn and Kenichi Yokoyama日期:04/20/2021,浏览量:34,Q&A: 0

    (1,3)-β-d-Glucan synthase (GS) is an essential enzyme for fungal cell wall biosynthesis that catalyzes the synthesis of (1,3)-β-d-glucan, a major and vital component of the cell wall. GS is a proven target of antifungal antibiotics

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    Optimized Recombinant Production of Secreted Proteins Using Human Embryonic Kidney (HEK293) Cells Grown in Suspension
    利用悬浮培养的人胚胎肾(HEK293)细胞优化分泌蛋白的重组生产

    Recombinant proteins are an essential milestone for a plethora of different applications ranging from pharmaceutical to clinical, and mammalian cell lines are among the currently preferred systems to obtain large amounts of proteins of interest due

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    Optimized Recombinant Production of Secreted Proteins Using Human Embryonic Kidney (HEK293) Cells Grown in Suspension
    利用悬浮培养的人胚胎肾(HEK293)细胞优化分泌蛋白的重组生产
    [Abstract]

    Recombinant proteins are an essential milestone for a plethora of different applications ranging from pharmaceutical to clinical, and mammalian cell lines are among the currently preferred systems to obtain large amounts of proteins of interest due to their high level of post-translational modification and manageable large-scale production. In

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    Quantitative Characterization of the Amount and Length of (1,3)-β-D-glucan for Functional and Mechanistic Analysis of Fungal (1,3)-β-D-glucan Synthase
    通过(1,3)-β-d-葡聚糖量和长度的定量表征对真菌(1,3)-β-d-葡聚糖合酶进行功能和机理分析
    作者:Abhishek Chhetri, Anna Loksztejn and Kenichi Yokoyama日期:04/20/2021,浏览量:34,Q&A: 0
    [Abstract]

    (1,3)-β-d-Glucan synthase (GS) is an essential enzyme for fungal cell wall biosynthesis that catalyzes the synthesis of (1,3)-β-d-glucan, a major and vital component of the cell wall. GS is a proven target of antifungal antibiotics including FDA-approved echinocandin derivatives; however, the function and mechanism of GS remain largely

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    Reconstitution of Chromatin by Stepwise Salt Dialysis
    逐步盐透析重组染色质
    作者:Grisel Cruz-Becerra and James T. Kadonaga日期:04/05/2021,浏览量:192,Q&A: 0
    [Abstract]

    Chromatin, rather than plain DNA, is the natural substrate of the molecular machines that mediate DNA-directed processes in the nucleus. Chromatin can be reconstituted in vitro by using different methodologies. The salt dialysis method yields chromatin that consists of purified histones and DNA. This biochemically pure chromatin is well-suited for

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    FRET-based Microscopy Assay to Measure Activity of Membrane Amino Acid Transporters with Single-transporter Resolution
    用荧光共振能量转移的显微技术测定单转运体膜氨基酸转运体的活性
    [Abstract]

    Secondary active transporters reside in cell membranes transporting polar solutes like amino acids against steep concentration gradients, using electrochemical gradients of ions as energy sources. Commonly, ensemble-based measurements of radiolabeled substrate uptakes or transport currents inform on kinetic parameters of transporters. Here we

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    Atomic Force Microscopy to Characterize Ginger Lipid-Derived Nanoparticles (GLDNP)
    原子力显微镜研究生姜脂质纳米颗粒(GLDNP)
    作者:Dingpei Long, Chunhua Yang, Junsik Sung and Didier Merlin日期:04/05/2021,浏览量:199,Q&A: 0
    [Abstract]

    We have demonstrated that a specific population of ginger-derived nanoparticles (GDNP-2) could effectively target the colon, reduce colitis, and alleviate colitis-associated colon cancer. Naturally occurring GDNP-2 contains complex bioactive components, including lipids, proteins, miRNAs, and ginger secondary metabolites (gingerols and shogaols).

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    Imaging Microtubules in vitro at High Resolution while Preserving their Structure
    保留微管结构同时在体外对其进行高分辨率成像
    作者:Camille Cuveillier, Yasmina Saoudi, Isabelle Arnal and Christian Delphin日期:04/05/2021,浏览量:204,Q&A: 0
    [Abstract]

    Microtubules (MT) are the most rigid component of the cytoskeleton. Nevertheless, they often appear highly curved in the cellular context and the mechanisms governing their overall shape are poorly understood. Currently, in vitro microtubule analysis relies primarily on electron microscopy for its high resolution and Total Internal Reflection

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    In vitro Reconstitution Assays of Arabidopsis 20S Proteasome
    拟南芥20S蛋白酶体的体外重组分析
    作者:Yanjun Li, Di Sun, Xingxing Yan, Zhiye Wang and Xiuren Zhang日期:04/05/2021,浏览量:203,Q&A: 0
    [Abstract]

    The majority of cellular proteins are degraded by the 26S proteasome in eukaryotes. However, intrinsically disordered proteins (IDPs), which contain large portions of unstructured regions and are inherently unstable, are degraded via the ubiquitin-independent 20S proteasome. Emerging evidence indicates that plant IDP homeostasis may also be

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    Expression and Purification of the Human Cation-chloride Cotransporter KCC1 from HEK293F Cells for Structural Studies
    从HEK293F细胞中人K-Cl转运蛋白KCC1的表达和纯化用于结构研究
    作者:Si Liu and Jiangtao Guo日期:04/05/2021,浏览量:201,Q&A: 0
    [Abstract]

    Cation-chloride cotransporters (CCCs) mediate the coupled, electroneutral symport of cations such as Na+ and/or K+ with chloride across membrane. Among CCCs family, K-Cl cotransporters (KCC1-KCC4) extrude intracellular Cl- by the transmembrane K+ gradient. In humans, these KCCs play vital roles in the physiology of the nervous system and kidney.

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    Quantitative Measurement of Mucolytic Enzymes in Fecal Samples
    粪便粘液溶解酶的定量测定
    作者:Shahanshah Khan and Hasan Zaki日期:03/20/2021,浏览量:753,Q&A: 0
    [Abstract]

    The mucus layer in the gastrointestinal tract covers the apical surface of intestinal epithelial cells, protecting the mucosal tissue from enteric pathogen and commensal microorganisms. The mucus is primarily composed of glycosylated protein called mucins, which are produced by goblet cells, a type of columnar epithelial cells in the intestinal

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    Monitoring Real-time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism
    利用荧光共振能量转移和圆二色性监测短发夹RNA的实时温度动态
    作者:Martin Balcerowicz, Marco Di Antonio and Betty Y. W. Chung日期:03/20/2021,浏览量:1027,Q&A: 0
    [Abstract]

    RNA secondary structures are highly dynamic and subject to prompt changes in response to the environment. Temperature in particular has a strong impact on RNA structural conformation, and temperature-sensitive RNA hairpin structures have been exploited by multiple organisms to modify the rate of translation in response to temperature changes.

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