生物化学

分类

    现刊
    Tyramide Signal-Amplified Immunofluorescence of MYCN and MYC in Human Tissue Specimens and Cell Line Cultures
    酪胺信号放大的人体组织标本和细胞系培养物中的MYCN和MYC免疫荧光
    作者:Johanna M. Schafer and Jennifer A. Pietenpol日期:07/05/2020,浏览量:0,Q&A: 0
    MYC family members, MYC, MYCN, and MYCL, are oncogenic transcription factors that regulate the expression of genes involved in normal development, cell growth, proliferation, metabolism, and survival. While MYC is amplified and/or overexpressed ...
    Preparation of Drosophila Polytene Chromosomes, Followed by Immunofluorescence Analysis of Chromatin Structure by Multi-fluorescence Correlations
    制备果蝇多线染色体并通过多重荧光关联对染色质结构进行免疫荧光分析
    作者:Terra M. Kuhn, Shawn C. Little and Maya Capelson日期:07/05/2020,浏览量:0,Q&A: 0
    Drosophila larval salivary gland polytene chromosome squashes have been used for decades to analyze genome-wide protein-binding patterns, transcriptional activation processes, and changes in chromatin structure at specific genetic loci. ...
    In vitro Assessment of Pathogen Effector Binding to Host Proteins by Surface Plasmon Resonance
    利用表面等离子体共振技术评价病原菌与宿主蛋白的结合
    作者:Marina Franceschetti, Mark J. Banfield, Clare E. M. Stevenson and Juan Carlos De la Concepcion日期:07/05/2020,浏览量:73,Q&A: 0
    The mechanisms of virulence and immunity are often governed by molecular interactions between pathogens and host proteins. The study of these interactions has major implications on understanding virulence activities, and how the host immune system ...
    Protocol for Peptide Synthesis on Spectrally Encoded Beads for MRBLE-pep Assays
    用于MRBLE-pep分析的光谱编码微球肽合成方案
    作者:Jamin B. Hein, Huy Q. Nguyen, Martha Cyert and Polly M. Fordyce日期:07/05/2020,浏览量:0,Q&A: 0
    Every living cell relies on signal transduction pathways comprised of protein-protein interactions (PPIs). In many cases, these PPIs are between a folded protein domain and a short linear motif (SLiM) within an unstructured region of a protein. As a ...
    Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis
    哺乳动物细胞脂筏的分离及其脂质组学分析
    作者:Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J. Meikle and Dmitri Sviridov日期:07/05/2020,浏览量:0,Q&A: 0
    Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways ...
    Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces
    小鼠粪便中短链脂肪酸的气相色谱检测
    作者:Chaozheng Zhang, An Liu, Tianshuang Zhang, Yi Li and Hua Zhao日期:07/05/2020,浏览量:15,Q&A: 0
    Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called ...
    Preparation of Yeast tRNA Sample for NMR Spectroscopy
    酵母tRNA样品的核磁共振制备
    作者:Marjorie Catala, Alexandre Gato, Carine Tisné and Pierre Barraud日期:06/20/2020,浏览量:453,Q&A: 0
    Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional modifications during their biosynthesis. To fulfil their functions within cells, tRNAs undergo a tightly controlled biogenesis process leading to the formation of mature tRNAs. In ...
    Assessments of HLA-I Specificities of Anti-HLA-I Monoclonal Antibodies Using Solid Phase Bead Arrays
    固相微珠技术评价抗HLA-I单克隆抗体的HLA-I特异性
    作者:Anita J. Zaitouna, Daniel S. Ramon and Malini Raghavan日期:06/20/2020,浏览量:432,Q&A: 0
    Human leukocyte antigen class I (HLA-I) molecules are a group of structurally-related cell surface proteins with a high degree of variability within the population. While only up to six variants are expressed in an individual person, the whole ...
    Electrophoretic Mobility Shift Assay of in vitro Phosphorylated RNA Polymerase II Carboxyl-terminal Domain Substrates
    体外磷酸化的RNA聚合酶II羧基末端域底物的电泳迁移率测定
    作者:Joshua E. Mayfield, Seema Irani and Yan Zhang日期:06/20/2020,浏览量:641,Q&A: 0
    Eukaryotic RNA polymerase II transcribes all protein-coding mRNAs and is highly regulated. A key mechanism directing RNA polymerase II and facilitating the co-transcriptional processing of mRNAs is the phosphorylation of its highly repetitive ...
    Identification of Buffer Conditions for Optimal Thermostability and Solubility of Herpesviral Protein UL37 Using the Thermofluor Assay
    用热荧光法鉴定疱疹病毒蛋白UL37的最佳热稳定性和溶解度的缓冲液条件
    作者:Andrea L. Koenigsberg, Jared D. Pitts and Ekaterina E. Heldwein日期:06/20/2020,浏览量:312,Q&A: 0
    Structural and biochemical studies of proteins require high amounts of stable, purified proteins. Protein stability often depends on the buffer composition, which includes pH and concentration of salts or other solutes such as glycerol, hence an ...
    Quantification of Protein Kinase A (PKA) Activity by An in vitro Radioactive Assay Using the Mouse Sperm Derived Enzyme
    用小鼠精子衍生酶体外放射性测定蛋白激酶A(PKA)活性
    作者:Cintia Stival, Carolina Baro Graf, Pablo E. Visconti and Dario Krapf日期:06/20/2020,浏览量:563,Q&A: 0
    In order to acquire fertilizing potential, mammalian sperm must undergo a process known as capacitation, which relies on the early activation of Protein Kinase A (PKA). Frequently, PKA activity is assessed in whole-cell ...
    In vitro Crosslinking Reactions and Substrate Incorporation Assays for The Identification of Transglutaminase-2 Protein Substrates
    转谷氨酰胺酶-2蛋白底物的体外交联反应和底物掺入鉴定
    作者:William L. Willis, Abigail Foster, Caitlin Henry, Lai Chu Wu and Wael Jarjour日期:06/20/2020,浏览量:453,Q&A: 0
    Transglutaminase (TG2) catalyzes protein crosslinking between glutamyl and lysyl residues. Catalytic activity occurs via a transamidation mechanism resulting in the formation of isopeptide bonds. Since TG2-mediated transamidation is of mechanistic ...
    Preparation of HeLa Total Membranes and Assay of Lipid-inhibition of Serine Palmitoyltransferase Activity
    HeLa全膜的制备及其对丝氨酸-棕榈酰转移酶活性的抑制作用
    作者:Muthukumar Kannan, Deanna L. Davis, John Suemitsu, Christopher D. Oltorik and Binks Wattenberg日期:06/20/2020,浏览量:524,Q&A: 0
    Serine palmitoyltranferase (SPT) is a pyridoxal 5′ phosphate (PLP)-dependent enzyme that catalyzes the first and rate-limiting step of de novo synthesis of sphingolipids. SPT activity is homeostatically regulated in response to increased ...
    Isolation of Tumor Cells Based on Their Distance from Blood Vessels
    基于与血管距离的肿瘤细胞分离
    作者:Saran Kumar, Husni Sharife, Tirzah Kreisel, Libat Bar-Lev, Myriam Grunewald and Eli Keshet日期:05/20/2020,浏览量:787,Q&A: 0
    Differential exposure of tumor cells to microenvironmental cues greatly impacts cell phenotypes, raising a need for position based sorting of tumor cells amenable to multiple OMICs and functional analyses. One such key determinant of tumor ...
    Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation
    重组输入蛋白-组蛋白配合物分子大小的超离心分析
    作者:Abhilash Padavannil, Chad A. Brautigam and Yuh Min Chook日期:05/20/2020,浏览量:520,Q&A: 0
    Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone ...
    Evaluation of the Efficiency of Genome Editing Tools by a Frameshift Fluorescence Protein Reporter
    通过移码荧光蛋白报告基因评估基因组编辑工具的效率
    作者:Balaji T. Moorthy, Akhilesh Kumar, Lauren X. Lotenfoe and Fangliang Zhang日期:05/20/2020,浏览量:760,Q&A: 0
    In the last decade, genome editing has been the center of attention as a novel tool for mechanistic investigations and for potential clinical applications. Various genome editing tools like meganucleases, zinc finger nucleases (ZFNs), transcription ...
    Spatial Image Correlation Spectroscopy (ICS): A Technique for Average Size Determination of Subcellular Accumulated Structures from Fluorescence Microscopic Images
    空间图像相关光谱法(ICS):一种从荧光显微图像中确定亚细胞聚集结构平均尺寸的技术
    作者:Akira Kitamura and Masakata Kinjo日期:05/20/2020,浏览量:630,Q&A: 0
    Size determination of subcellular structures such as inclusion bodies (IBs) and granules from fluorescent images is important for identification and structural characterization. However, it is often time-consuming just for the comparison of the ...
    Tyramide Signal-Amplified Immunofluorescence of MYCN and MYC in Human Tissue Specimens and Cell Line Cultures
    酪胺信号放大的人体组织标本和细胞系培养物中的MYCN和MYC免疫荧光
    作者:Johanna M. Schafer and Jennifer A. Pietenpol日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] MYC family members, MYC, MYCN, and MYCL, are oncogenic transcription factors that regulate the expression of genes involved in normal development, cell growth, proliferation, metabolism, and survival. While MYC is amplified and/or overexpressed across a variety of tissue types, MYCN is often overexpressed in tumors of the nervous system ...
    Preparation of Drosophila Polytene Chromosomes, Followed by Immunofluorescence Analysis of Chromatin Structure by Multi-fluorescence Correlations
    制备果蝇多线染色体并通过多重荧光关联对染色质结构进行免疫荧光分析
    作者:Terra M. Kuhn, Shawn C. Little and Maya Capelson日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] Drosophila larval salivary gland polytene chromosome squashes have been used for decades to analyze genome-wide protein-binding patterns, transcriptional activation processes, and changes in chromatin structure at specific genetic loci. There have been many evolutions of the squashing protocol over the years, with sub-optimal ...
    In vitro Assessment of Pathogen Effector Binding to Host Proteins by Surface Plasmon Resonance
    利用表面等离子体共振技术评价病原菌与宿主蛋白的结合
    作者:Marina Franceschetti, Mark J. Banfield, Clare E. M. Stevenson and Juan Carlos De la Concepcion日期:07/05/2020,浏览量:73,Q&A: 0
    [Abstract] The mechanisms of virulence and immunity are often governed by molecular interactions between pathogens and host proteins. The study of these interactions has major implications on understanding virulence activities, and how the host immune system recognizes the presence of pathogens to initiate an immune response. Frequently, the association ...
    Protocol for Peptide Synthesis on Spectrally Encoded Beads for MRBLE-pep Assays
    用于MRBLE-pep分析的光谱编码微球肽合成方案
    作者:Jamin B. Hein, Huy Q. Nguyen, Martha Cyert and Polly M. Fordyce日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] Every living cell relies on signal transduction pathways comprised of protein-protein interactions (PPIs). In many cases, these PPIs are between a folded protein domain and a short linear motif (SLiM) within an unstructured region of a protein. As a result of this small interaction interface (3-10 amino acids), the affinities of SLiM-mediated ...
    Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis
    哺乳动物细胞脂筏的分离及其脂质组学分析
    作者:Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J. Meikle and Dmitri Sviridov日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and ...
    Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces
    小鼠粪便中短链脂肪酸的气相色谱检测
    作者:Chaozheng Zhang, An Liu, Tianshuang Zhang, Yi Li and Hua Zhao日期:07/05/2020,浏览量:15,Q&A: 0
    [Abstract] Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called short-chain fatty acids. SCFAs are closely related to various aspects of the human body, so more and ...
    Preparation of Yeast tRNA Sample for NMR Spectroscopy
    酵母tRNA样品的核磁共振制备
    作者:Marjorie Catala, Alexandre Gato, Carine Tisné and Pierre Barraud日期:06/20/2020,浏览量:453,Q&A: 0
    [Abstract] Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional modifications during their biosynthesis. To fulfil their functions within cells, tRNAs undergo a tightly controlled biogenesis process leading to the formation of mature tRNAs. In addition, functions of tRNAs are often modulated by their modifications. Although the biological ...
    Assessments of HLA-I Specificities of Anti-HLA-I Monoclonal Antibodies Using Solid Phase Bead Arrays
    固相微珠技术评价抗HLA-I单克隆抗体的HLA-I特异性
    作者:Anita J. Zaitouna, Daniel S. Ramon and Malini Raghavan日期:06/20/2020,浏览量:432,Q&A: 0
    [Abstract] Human leukocyte antigen class I (HLA-I) molecules are a group of structurally-related cell surface proteins with a high degree of variability within the population. While only up to six variants are expressed in an individual person, the whole population contains thousands of different variants. The ability to distinguish specific variants is ...
    Electrophoretic Mobility Shift Assay of in vitro Phosphorylated RNA Polymerase II Carboxyl-terminal Domain Substrates
    体外磷酸化的RNA聚合酶II羧基末端域底物的电泳迁移率测定
    作者:Joshua E. Mayfield, Seema Irani and Yan Zhang日期:06/20/2020,浏览量:641,Q&A: 0
    [Abstract] Eukaryotic RNA polymerase II transcribes all protein-coding mRNAs and is highly regulated. A key mechanism directing RNA polymerase II and facilitating the co-transcriptional processing of mRNAs is the phosphorylation of its highly repetitive carboxyl-terminal domain (CTD) of its largest subunit, RPB1, at specific residues. A variety of techniques ...
    Identification of Buffer Conditions for Optimal Thermostability and Solubility of Herpesviral Protein UL37 Using the Thermofluor Assay
    用热荧光法鉴定疱疹病毒蛋白UL37的最佳热稳定性和溶解度的缓冲液条件
    作者:Andrea L. Koenigsberg, Jared D. Pitts and Ekaterina E. Heldwein日期:06/20/2020,浏览量:312,Q&A: 0
    [Abstract] Structural and biochemical studies of proteins require high amounts of stable, purified proteins. Protein stability often depends on the buffer composition, which includes pH and concentration of salts or other solutes such as glycerol, hence an efficient method for identifying optimal buffer conditions for stability would minimize time and ...