生物化学

分类

    现刊
    Cell-based Assay for Recruitment of DDR1 to Collagen-coated Beads
    以细胞为基础的DDR1向胶原包被微珠的招募分析
    作者:Victoria Juskaite and Birgit Leitinger日期:08/20/2019,浏览量:43,Q&A: 0
    The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for ...
    A Radioactive in vitro ERK3 Kinase Assay
    体外ERK3激酶的放射性分析
    作者:Lobna Elkhadragy and Weiwen Long日期:08/20/2019,浏览量:55,Q&A: 0
    Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases that have an important role in signal transduction. Extracellular signal-regulated kinase 3 (ERK3), also known as MAPK6, is an atypical MAPK. Here, we describe in detail an in ...
    Peptide Feeding and Mechanical Wounding for Tomato Seedlings
    番茄幼苗的肽饲喂和机械伤害
    作者:Lei Wang, Yan Wang and Georg Felix日期:03/20/2019,浏览量:1338,Q&A: 0
    Plants need to respond appropriately to wounding and herbivorous insects. Peptide signals have been implicated in local and systemic induction of appropriate plant defense responses. To study these peptide signals and their perception in host ...
    Biofilm Assays on Fibrinogen-coated Silicone Catheters and 96-well Polystyrene Plates
    在纤维蛋白原包覆的硅胶导管和96孔聚苯乙烯板上进行生物膜检测
    作者:Cristina Colomer-Winter, José A. Lemos and Ana L. Flores-Mireles日期:03/20/2019,浏览量:1094,Q&A: 0
    Biofilm formation is a well-known bacterial strategy that protects cells from hostile environments. During infection, bacteria found in a biofilm community are less sensitive to antibiotics and to the immune response, often allowing them to colonize ...
    Cell-based Assay for Recruitment of DDR1 to Collagen-coated Beads
    以细胞为基础的DDR1向胶原包被微珠的招募分析
    作者:Victoria Juskaite and Birgit Leitinger日期:08/20/2019,浏览量:43,Q&A: 0
    [Abstract] The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for in vitro studies. Thus, in vitro studies often use acid-soluble collagens in the ...
    A Radioactive in vitro ERK3 Kinase Assay
    体外ERK3激酶的放射性分析
    作者:Lobna Elkhadragy and Weiwen Long日期:08/20/2019,浏览量:55,Q&A: 0
    [Abstract] Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases that have an important role in signal transduction. Extracellular signal-regulated kinase 3 (ERK3), also known as MAPK6, is an atypical MAPK. Here, we describe in detail an in vitro assay for the kinase activity of ERK3 using myelin basic protein (MBP) or steroid ...
    Cell Type-specific mRNA Purification in Caenorhabditis elegans via Translating Ribosome Affinity Purification
    秀丽隐杆线虫细胞特异性mRNA的核糖体亲和纯化
    作者:Ian G. McLachlan and Steven W. Flavell日期:08/05/2019,浏览量:261,Q&A: 0
    [Abstract] Cell type-specific molecular profiling is widely used to gain new insights into the diverse cell types that make up complex biological tissues. Translating ribosome affinity purification (TRAP) is a method in which the cell type-specific expression of epitope-tagged ribosomal subunits allows one to purify actively translating mRNAs without the ...
    Measuring Protein Half-life in Arabidopsis thaliana
    拟南芥蛋白半衰期的检测
    作者:Hongqing Guo and Yanhai Yin日期:08/05/2019,浏览量:200,Q&A: 0
    [Abstract] Post-translational modifications play important roles in controlling protein function and can lead to altered protein stability. Protein stability can be determined after treatment with the protein synthesis inhibitor Cycloheximide. Cycloheximide is a translational inhibitor that inhibits protein synthesis via cytoplasmic ribosomes. Here we ...
    Quantification of Prostaglandin E2 Concentration in Interstitial Fluid from the Hypothalamic Region of Free-moving Mice
    "自由活动小鼠下丘脑区域间质液中前列腺素E2浓度的定量分析
    作者:Yoshinobu Nakamura, Takatoshi Sakaguchi, Ikumi Tamai and Takeo Nakanishi日期:08/05/2019,浏览量:217,Q&A: 0
    [Abstract] Prostaglandin E2 (PGE2) is a well-established chemical mediator for the generation of the fever at the hypothalamus of the brain. PGE2 mediates fever generation via PGE receptor 3 (i.e., EP3) on neurons in the preoptic area. The role of PGE2 has been analyzed by measuring PGE2 ...
    In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay
    通过体外RNA剪切检测IRE1依赖的RNA降解
    作者:G. Elif Karagöz, Jirka Peschek, Peter Walter and Diego Acosta-Alvear日期:07/20/2019,浏览量:854,Q&A: 0
    [Abstract] The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube. Here we describe a protocol for the in vitro cleavage and analysis of RNA substrates of IRE1. The method consists of the in vitro transcription, purification ...
    Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina
    等电聚焦用于小鼠视网膜中磷酸化视紫红质的定量分析
    作者:Sowmya Bekshe Lokappa, M. Carter Cornwall and Jeannie Chen日期:07/20/2019,浏览量:1154,Q&A: 0
    [Abstract] Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its ability to activate the visual G-protein transducin. Binding of light-activated, ...
    Evaluation of Genotoxicity by Micronucleus Assay in vitro and by Allium cepa Test in vivo
    利用微核体外分析系统和洋葱体内实验进行遗传毒性测试
    作者:Christina N. Banti and Sotiris K. Hadjikakou日期:07/20/2019,浏览量:587,Q&A: 0
    [Abstract] The in vitro and in vivo genotoxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) is evaluated by the micronucleus test and the Allium cepa assay, respectively. Fetal lung fibroblast cells (MRC-5), normal human corneal epithelial cells (HCEC) and ...
    Adhesive and Cohesive Peel Force Measurement of Human Airway Mucus
    人气道粘液的黏附力和粘结剥离力测定
    [Abstract] In health, the high-speed airflow associated with cough represents a vital backup mechanism for clearing accumulated mucus from our airways. However, alterations in the mucus layer in cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) leads to the mucus layer adhered to the airway surfaces, representing the nidus of chronic lung ...
    Oxygen Consumption Measurements in Caenorhabditis elegans Using the Seahorse XF24
    利用海马细胞能量代谢实时测定仪XF24 进行秀丽隐杆线虫中耗氧量测定
    作者:Suraiya Haroon and Marc Vermulst日期:07/05/2019,浏览量:453,Q&A: 0
    [Abstract] Mitochondria generate 90% of the energy required to sustain life. As a result, loss of mitochondrial function compromises almost every facet of human physiology. Accordingly, most mitochondrial diseases tend to present themselves as complex, multi-systemic disorders that can be difficult to diagnose. Depending on the severity of the mitochondrial ...