生物化学

分类

    现刊
    A Highly Sensitive, Reproducible Assay for Determining 4-hydroxynonenal Protein Adducts in Biological Material
    生物材料中4-羟基壬烯醛与蛋白质加合物的高灵敏、可重复检测法
    作者:T. Blake Monroe and Ethan J. Anderson日期:10/05/2019,浏览量:142,Q&A: 0
    Oxidative stress is associated with numerous diseases, and markers of oxidative stress in biological material are becoming a mainstay of both experimental and clinical/epidemiological research. Lipid peroxidation is a major form of oxidative stress, ...
    Isolation, Purification, and Characterization of Ginger-derived Nanoparticles (GDNPs) from Ginger, Rhizome of Zingiber officinale
    源自生姜及根茎的生姜源性纳米颗粒(GDNPs)的分离、纯化和鉴定
    作者:Junsik Sung, Chunhua Yang, Emilie Viennois, Mingzhen Zhang and Didier Merlin日期:10/05/2019,浏览量:201,Q&A: 0
    Factors implicated in the pathophysiology of intestinal inflammation include defects in intestinal epithelial barrier function, abnormal immune responses, and activities of the gut microbiota. Current agents used to treat human Inflammatory Bowels ...
    Measurement of Acid Ecto-phosphatase Activity in Live Leishmania donovani Parasites
    杜氏利什曼原虫中酸性胞外磷酸酶活性的活体检测
    作者:Amalia Papadaki and Haralabia Boleti日期:10/05/2019,浏览量:111,Q&A: 0
    Acid ecto-phosphatases are enzymes that hydrolyze phosphomonoesters in the acidic pH range with their active sites facing the extacellular medium. Their activities can be measured in living cells. In bacteria and protozoan pathogens, acid ...
    Conjugation of Fab’ Fragments with Fluorescent Dyes for Single-molecule Tracking on Live Cells
    Fab片段结合荧光染料用于活细胞单分子示踪
    作者:I-Ting Teng, Xiangning Bu and Inhee Chung日期:09/20/2019,浏览量:241,Q&A: 0
    Our understanding of the regulation and functions of cell-surface proteins has progressed rapidly with the advent of advanced optical imaging techniques. In particular, single-molecule tracking (SMT) using bright fluorophores conjugated to ...
    Subcellular Fractionation of Hela Cells for Lysosome Enrichment Using a Continuous Percoll-density Gradient
    利用连续percoll密度梯度富集hela细胞溶酶体亚细胞组分
    作者:Julian M. Carosi, Kathryn J. Hattersley, Yi Cui, Zhe Yang, Rohan D. Teasdale and Timothy J. Sargeant日期:09/20/2019,浏览量:351,Q&A: 0
    The enrichment of lysosomes is a useful way to study their structure and function. These dynamic vesicles can be enriched from cell cultures in a variety of ways including immunoprecipitation and fluorescence-activated organelle sorting. These ...
    Supported Cell Membrane Sheets to Monitor Protein Assembly
    蛋白质组装的细胞膜片监测
    作者:Simon Erlendsson, Thor Seneca Thorsen and Kenneth Lindegaard Madsen日期:09/20/2019,浏览量:251,Q&A: 0
    Studying protein-protein and protein-lipid interactions in their native environment is highly desirable, yet, the heterogeneity and complexity of cellular systems limits the repertoire of experimental methods available. In cells, interactions are ...
    A PhoA-STII Based Method for Efficient Extracellular Secretion and Purification of Fab from Escherichia coli
    一种基于phoa stii技术的 大肠杆菌中高效分泌表达的fab片段纯化
    作者:Ziyan Wang, Yang Gao, Manyu Luo, Cedric Cagliero, Hua Jiang, Yueqing Xie, Jianwei Zhu and Huili Lu日期:09/20/2019,浏览量:200,Q&A: 0
    In comparison with full-length IgGs, antigen binding fragments (Fabs) are smaller in size and do not require the complexed post-translational modification. Therefore, Fab can be cost-effectively produced using an Escherichia coli (E. coli ...
    A Robust, One-step FRET Assay for Human Heparanase
    人乙酰肝素酶的一步快速FRET检测
    作者:Jyothi C. Sistla and Umesh R. Desai日期:09/05/2019,浏览量:296,Q&A: 0
    Heparanase, an endo-β-D-glucuronidase, cleaves cell surface and extracellular matrix heparan sulfate (HS) chains at distinct sites and plays important biological roles including modulation of cell growth and metastasis. Although a number of ...
    Photoaffinity Labeling of Respiratory Complex I in Bovine Heart Submitochondrial Particles by Photoreactive [125I] amilorides
    利用光敏阿米洛利光亲和标记牛心脏亚线粒体颗粒中呼吸链复合物I
    作者:Masatoshi Murai and Hideto Miyoshi日期:09/05/2019,浏览量:257,Q&A: 0
    The architecture of quinone/inhibitor-access channel in proton-translocating NADH-quinone oxidoreductase (respiratory complex I) was modeled by X-ray crystallography and cryo-EM, however, it remains debatable whether the channel model reflects the ...
    Cell-based Assay for Recruitment of DDR1 to Collagen-coated Beads
    以细胞为基础的DDR1向胶原包被微珠的招募分析
    作者:Victoria Juskaite and Birgit Leitinger日期:08/20/2019,浏览量:352,Q&A: 0
    The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for ...
    A Radioactive in vitro ERK3 Kinase Assay
    体外ERK3激酶的放射性分析
    作者:Lobna Elkhadragy and Weiwen Long日期:08/20/2019,浏览量:361,Q&A: 0
    Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases that have an important role in signal transduction. Extracellular signal-regulated kinase 3 (ERK3), also known as MAPK6, is an atypical MAPK. Here, we describe in detail an in ...
    Peptide Feeding and Mechanical Wounding for Tomato Seedlings
    番茄幼苗的肽饲喂和机械伤害
    作者:Lei Wang, Yan Wang and Georg Felix日期:03/20/2019,浏览量:1550,Q&A: 0
    Plants need to respond appropriately to wounding and herbivorous insects. Peptide signals have been implicated in local and systemic induction of appropriate plant defense responses. To study these peptide signals and their perception in host ...
    Biofilm Assays on Fibrinogen-coated Silicone Catheters and 96-well Polystyrene Plates
    在纤维蛋白原包覆的硅胶导管和96孔聚苯乙烯板上进行生物膜检测
    作者:Cristina Colomer-Winter, José A. Lemos and Ana L. Flores-Mireles日期:03/20/2019,浏览量:1330,Q&A: 0
    Biofilm formation is a well-known bacterial strategy that protects cells from hostile environments. During infection, bacteria found in a biofilm community are less sensitive to antibiotics and to the immune response, often allowing them to colonize ...
    A Highly Sensitive, Reproducible Assay for Determining 4-hydroxynonenal Protein Adducts in Biological Material
    生物材料中4-羟基壬烯醛与蛋白质加合物的高灵敏、可重复检测法
    作者:T. Blake Monroe and Ethan J. Anderson日期:10/05/2019,浏览量:142,Q&A: 0
    [Abstract] Oxidative stress is associated with numerous diseases, and markers of oxidative stress in biological material are becoming a mainstay of both experimental and clinical/epidemiological research. Lipid peroxidation is a major form of oxidative stress, but due to their rapid degradation and instability, lipid peroxides are notoriously difficult to ...
    Isolation, Purification, and Characterization of Ginger-derived Nanoparticles (GDNPs) from Ginger, Rhizome of Zingiber officinale
    源自生姜及根茎的生姜源性纳米颗粒(GDNPs)的分离、纯化和鉴定
    作者:Junsik Sung, Chunhua Yang, Emilie Viennois, Mingzhen Zhang and Didier Merlin日期:10/05/2019,浏览量:201,Q&A: 0
    [Abstract] Factors implicated in the pathophysiology of intestinal inflammation include defects in intestinal epithelial barrier function, abnormal immune responses, and activities of the gut microbiota. Current agents used to treat human Inflammatory Bowels Disease (IBD), chronic inflammation of digestive tract, have serious side effects. In addition, most ...
    Measurement of Acid Ecto-phosphatase Activity in Live Leishmania donovani Parasites
    杜氏利什曼原虫中酸性胞外磷酸酶活性的活体检测
    作者:Amalia Papadaki and Haralabia Boleti日期:10/05/2019,浏览量:111,Q&A: 0
    [Abstract] Acid ecto-phosphatases are enzymes that hydrolyze phosphomonoesters in the acidic pH range with their active sites facing the extacellular medium. Their activities can be measured in living cells. In bacteria and protozoan pathogens, acid ecto-phosphatases have been associated with the survival of intracellular pathogens within phagocytes through ...
    Conjugation of Fab’ Fragments with Fluorescent Dyes for Single-molecule Tracking on Live Cells
    Fab片段结合荧光染料用于活细胞单分子示踪
    作者:I-Ting Teng, Xiangning Bu and Inhee Chung日期:09/20/2019,浏览量:241,Q&A: 0
    [Abstract] Our understanding of the regulation and functions of cell-surface proteins has progressed rapidly with the advent of advanced optical imaging techniques. In particular, single-molecule tracking (SMT) using bright fluorophores conjugated to antibodies and wide-field microscopy methods such as total internal reflection fluorescence microscopy have ...
    Subcellular Fractionation of Hela Cells for Lysosome Enrichment Using a Continuous Percoll-density Gradient
    利用连续percoll密度梯度富集hela细胞溶酶体亚细胞组分
    作者:Julian M. Carosi, Kathryn J. Hattersley, Yi Cui, Zhe Yang, Rohan D. Teasdale and Timothy J. Sargeant日期:09/20/2019,浏览量:351,Q&A: 0
    [Abstract] The enrichment of lysosomes is a useful way to study their structure and function. These dynamic vesicles can be enriched from cell cultures in a variety of ways including immunoprecipitation and fluorescence-activated organelle sorting. These methods are extremely precise but often require the transfection and expression of an affinity or ...
    Supported Cell Membrane Sheets to Monitor Protein Assembly
    蛋白质组装的细胞膜片监测
    作者:Simon Erlendsson, Thor Seneca Thorsen and Kenneth Lindegaard Madsen日期:09/20/2019,浏览量:251,Q&A: 0
    [Abstract] Studying protein-protein and protein-lipid interactions in their native environment is highly desirable, yet, the heterogeneity and complexity of cellular systems limits the repertoire of experimental methods available. In cells, interactions are often taking place in confined microenvironments where factors such as avidity, hindered diffusion, ...
    A PhoA-STII Based Method for Efficient Extracellular Secretion and Purification of Fab from Escherichia coli
    一种基于phoa stii技术的 大肠杆菌中高效分泌表达的fab片段纯化
    作者:Ziyan Wang, Yang Gao, Manyu Luo, Cedric Cagliero, Hua Jiang, Yueqing Xie, Jianwei Zhu and Huili Lu日期:09/20/2019,浏览量:200,Q&A: 0
    [Abstract] In comparison with full-length IgGs, antigen binding fragments (Fabs) are smaller in size and do not require the complexed post-translational modification. Therefore, Fab can be cost-effectively produced using an Escherichia coli (E. coli) expression system. However, the disulfide-bonds containing exogenous protein, including ...
    A Robust, One-step FRET Assay for Human Heparanase
    人乙酰肝素酶的一步快速FRET检测
    作者:Jyothi C. Sistla and Umesh R. Desai日期:09/05/2019,浏览量:296,Q&A: 0
    [Abstract] Heparanase, an endo-β-D-glucuronidase, cleaves cell surface and extracellular matrix heparan sulfate (HS) chains at distinct sites and plays important biological roles including modulation of cell growth and metastasis. Although a number of different types of heparanase assays have been reported to date, most are labor intensive, complex ...
    Photoaffinity Labeling of Respiratory Complex I in Bovine Heart Submitochondrial Particles by Photoreactive [125I] amilorides
    利用光敏阿米洛利光亲和标记牛心脏亚线粒体颗粒中呼吸链复合物I
    作者:Masatoshi Murai and Hideto Miyoshi日期:09/05/2019,浏览量:257,Q&A: 0
    [Abstract] The architecture of quinone/inhibitor-access channel in proton-translocating NADH-quinone oxidoreductase (respiratory complex I) was modeled by X-ray crystallography and cryo-EM, however, it remains debatable whether the channel model reflects the physiologically relevant state present throughout the catalytic cycle. Using photoreactive [125 ...
    Cell-based Assay for Recruitment of DDR1 to Collagen-coated Beads
    以细胞为基础的DDR1向胶原包被微珠的招募分析
    作者:Victoria Juskaite and Birgit Leitinger日期:08/20/2019,浏览量:352,Q&A: 0
    [Abstract] The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for in vitro studies. Thus, in vitro studies often use acid-soluble collagens in the ...