生物化学

分类

    现刊
    Production of the Receptor-binding Domain of the Viral Spike Proteins from 2003 and 2019 SARS CoVs and the Four Common Human Coronaviruses for Serologic Assays and Inhibitor Screening
    从2003年和2019年SARS冠状病毒和四种常见人类冠状病毒的血清学分析和抑制剂筛选中产生病毒刺突蛋白受体结合域
    [Abstract]

    The recombinant receptor-binding domain (RBD) of the viral spike protein from SARS-CoV-1 and 2 are reliable antigens for detecting viral-specific antibodies in humans. We and others have shown that the levels of RBD-binding antibodies and SARS-CoV-2 neutralizing antibodies in patients are correlated. Here, we report the expression and purification

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    A Fluorescence Dequenching-based Liposome Leakage Assay to Measure Membrane Permeabilization by Pore-forming Proteins
    一种以测定成孔蛋白膜通透性的基于荧光淬灭的脂质体渗漏测定方法
    作者:Javier Aguilera, Salvador Vazquez-Reyes and Jianjun Sun日期:05/20/2021,浏览量:867,Q&A: 0
    [Abstract]

    Pore-forming toxins (PFTs) have been discovered in a wide range of organisms. Their functions are essential to the survival or virulence of many species. PFTs often interact with lipid membranes. Large unilamellar vesicles (LUV), also known as liposomes, have been commonly used as reliable membrane models for testing PFTs activity. Liposomes have

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    A New Method for Studying RNA-binding Proteins on Specific RNAs
    一种研究特异性RNA结合蛋白的新方法
    作者:Weiping Sun, Ziheng Zhang, Ji-Long Liu and Min Zhuang日期:05/20/2021,浏览量:1314,Q&A: 0
    [Abstract]

    Proximity-based protein labeling has been developed to identify protein-nucleic acid interactions. We have reported a novel method termed CRUIS (CRISPR-based RNA-United Interacting System), which captures RNA-protein interactions in living cells by combining the RNA-binding capacity of CRISPR/Cas13 and the proximity-tagging activity of PUP-IT.

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    Intracellular IRF5 Dimerization Assay
    细胞内IRF5二聚分析
    作者:Cherrie D. Sherman and Betsy J. Barnes日期:05/20/2021,浏览量:913,Q&A: 0
    [Abstract]

    The intracellular interferon regulatory factor 5 (IRF5) dimerization assay is a technique designed to measure molecular interaction(s) with endogenous IRF5. Here, we present two methods that detect endogenous IRF5 homodimerization and interaction of endogenous IR5 with cell penetrating peptide (CPP) inhibitors. Briefly, to detect endogenous IRF5

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    Cell-free Synthesis of Correctly Folded Proteins with Multiple Disulphide Bonds: Production of Fungal Hydrophobins
    有多重二硫化物键的正确折叠蛋白质的无细胞合成:真菌疏水性化合物的生产
    作者:Rezwan Siddiquee and Ann H Kwan日期:05/20/2021,浏览量:1144,Q&A: 0
    [Abstract]

    Cell-free synthesis is a powerful technique that uses the transcriptional and translational machinery extracted from cells to create proteins without the constraints of living cells. Here, we report a cell-free protein production protocol using Escherichia coli lysate (Figure 1) to successfully express a class of proteins (known as hydrophobins)

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    Single-Molecule Studies of Membrane Receptors from Brain Region Specific Nanovesicles
    脑区特异性纳米泡膜受体的单分子研究
    作者:Surya P. Aryal, Xu Fu, Abdullah A. Masud, Khaga R. Neupane and Christopher I. Richards日期:05/20/2021,浏览量:914,Q&A: 0
    [Abstract]

    Single molecule imaging and spectroscopy are powerful techniques for the study of a wide range of biological processes including protein assembly and trafficking. However, in vivo single molecule imaging of biomolecules has been challenging because of difficulties associated with sample preparation and technical challenges associated with

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    Characterising Plant Deubiquitinases with in vitro Activity-based Labelling and Ubiquitin Chain Disassembly Assays
    植物去泛素酶的体外活性标记和泛素链分解分析
    作者:Michael J. Skelly and Steven H. Spoel日期:05/05/2021,浏览量:475,Q&A: 0
    [Abstract]

    Post-translational modification of proteins by ubiquitin is an essential cellular signaling mechanism in all eukaryotes. Ubiquitin is removed from target proteins by a wide range of deubiquitinase (DUB) enzymes with different activities and substrate specificities. Understanding how DUBs function in vitro is a vital first step to uncovering their

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    Chromatographic Assays for the Enzymatic Degradation of Chitin
    几丁质酶解的色谱分析
    作者:Sophanit Mekasha, Tina R. Tuveng, Gustav Vaaje-Kolstad and Vincent G. H. Eijsink日期:05/05/2021,浏览量:544,Q&A: 0
    [Abstract]

    Chitin is an insoluble linear polymer of β(1→4)-linked N-acetylglucosamine. Enzymatic cleavage of chitin chains can be achieved using hydrolytic enzymes, called chitinases, and/or oxidative enzymes, called lytic polysaccharide monooxygenases (LPMOs). These two groups of enzymes have different modes of action and yield different product

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    Histological Methods to Detect Early-stage Plant Defense Responses during Artificial Inoculation of Lolium perenne with Epichloë festucae
    羊茅人工接种多年生黑麦草过程中植物早期防御反应的组织学检测
    作者:Mostafa Rahnama, Damien J. Fleetwood and Richard D. Johnson日期:05/05/2021,浏览量:522,Q&A: 0
    [Abstract]

    Epichloë species form agriculturally important symbioses with many cool season grasses. To study these symbioses, such as the interaction of Epichloë festucae with perennial ryegrass (Lolium perenne), host plants can be infected by artificial inoculation of etiolated seedlings. This inoculation is performed by placing mycelium into an

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    COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR
    COVID-19样本汇集:从RNA提取到实时定量RT-PCR
    [Abstract]

    The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume

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