• 卷册 11, 2021
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Image Credit: BioProtoc.4054

往期刊物(2021)

卷册: 11 期号: 12

生物化学

Protein Import Assay into Mitochondria Isolated from Human Cells 从人细胞中分离的线粒体蛋白质输入测定 作者:Lena M. Murschall, Esra Peker, Thomas MacVicar, Thomas Langer and Jan Riemer日期:06/20/2021,浏览量:1456,Q&A: 0

Mitochondria are essential organelles containing approximately 1,500 proteins. Only approximately 1% of these proteins are synthesized inside mitochondria, whereas the remaining 99% are synthesized as precursors on cytosolic ribosomes and imported into the organelle. Various tools and techniques to analyze the import process have been developed.

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Enrichment of Vascular Fragments from Mouse Embryonic Brains for Endothelial Cell Analysis 用于内皮细胞分析小鼠胚胎脑血管片段富集 作者:Nicolas Santander and Thomas D. Arnold日期:06/20/2021,浏览量:1083,Q&A: 0

Endothelial cells in the brain interact with other cell types, forming the blood-brain barrier. This barrier controls the movement of solutes into and out of the brain, regulating pathophysiological processes and drug delivery to the brain. Common isolation methods used to study these cells during embryonic development involve enzymatic treatment

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生物医学工程

A Detailed Protocol for Preparing Millimeter-sized Supergiant Liposomes that Permit Efficient Eukaryotic Cell-free Translation in the Interior 一种制备毫米大小的允许在内部有效的真核细胞游离翻译的超巨大脂质体的详细方案 作者:Hajime Takahashi and Atsushi Ogawa日期:06/20/2021,浏览量:887,Q&A: 0

Liposomes have been used as a pseudo cell membrane for encapsulating biomolecules and creating an artificial cell in the interior where biochemical reactions can occur. Among the several methods used to prepare biomolecule-encapsulating liposomes, the spontaneous emulsion transfer method is superior to others in that it allows us to readily

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细胞生物学

Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening 基于荧光的全染色单细胞分析和清除的微组织和类器官高通量筛选 作者:Henrik Renner, Mandy Otto, Martha Grabos, Hans R. Schöler and Jan M. Bruder日期:06/20/2021,浏览量:1481,Q&A: 1

Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues (“organoids”), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically, tissue sectioning is the standard method for immunohistochemical analysis. However, it removes cells

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Electrophysiological Properties of Neurons: Current-Clamp Recordings in Mouse Brain Slices and Firing-Pattern Analysis 神经元的电生理特性:小鼠脑切片中的电流钳记录和放电模式分析 作者:Elina Nagaeva, Ivan Zubarev and Esa R. Korpi日期:06/20/2021,浏览量:1443,Q&A: 0

Characterization of an electrically active cell, such as a neuron, demands measurement of its electrical properties. Due to differences in gene activation, location, innervation patterns, and functions, the millions of neurons in the mammalian brain are tremendously diverse in their membrane characteristics and abilities to generate action

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免疫学

A Lipidomics Approach to Measure Phosphatidic Acid Species in Subcellular Membrane Fractions Obtained from Cultured Cells 用脂质组学方法测定培养细胞亚细胞膜组分中磷脂酸的种类 作者:Nawal Kassas, Laetitia Fouillen, Stéphane Gasman and Nicolas Vitale日期:06/20/2021,浏览量:1139,Q&A: 0

Over the last decade, lipids have emerged as possessing an ever-increasing number of key functions, especially in membrane trafficking. For instance, phosphatidic acid (PA) has been proposed to play a critical role in different steps along the secretory pathway or during phagocytosis. To further investigate in detail the precise nature of PA

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微生物学

Fluorescence-based Heme Quantitation in Toxoplasma Gondii 基于荧光的弓形虫血红素定量 作者:Amy Bergmann and Zhicheng Dou日期:06/20/2021,浏览量:1052,Q&A: 0

Toxoplasma gondii is a highly prevalent protozoan pathogen throughout the world. As a eukaryotic intracellular pathogen, Toxoplasma ingests nutrients from host cells to support its intracellular growth. The parasites also encode full or partial metabolic pathways for the biosynthesis of certain nutrients, such as heme. Heme is an essential

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Transcriptional Run-on: Measuring Nascent Transcription at Specific Genomic Sites in Yeast 连缀转录:测定酵母特定基因组位点的新生转录 作者:Victoria Begley, Lola de Miguel-Jiménez and Sebastián Chávez日期:06/20/2021,浏览量:899,Q&A: 0

DNA transcription by RNA polymerases has always interested the scientific community as it is one of the most important processes involved in genome expression. This has led scientists to come up with different protocols allowing analysis of this process in specific locations across the genome by quantitating the amount of RNA polymerases

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神经科学

In vivo Optical Access to Olfactory Sensory Neurons in the Mouse Olfactory Epithelium 小鼠嗅上皮嗅觉感觉神经元的体内光学通路研究 作者:Shigenori Inagaki, Ryo Iwata and Takeshi Imai日期:06/20/2021,浏览量:1198,Q&A: 0

In neuroscience, it is fundamental to understand how sensory stimuli are translated into neural activity at the entry point of sensory systems. In the olfactory system, odorants inhaled into the nasal cavity are detected by ~1,000 types of odorant receptors (ORs) that are expressed by olfactory sensory neurons (OSNs). Since each OSN expresses only

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Intravenous and Non-invasive Drug Delivery to the Mouse Basal Forebrain Using MRI-guided Focused Ultrasound MRI引导下聚焦超声对小鼠基底前脑静脉及无创给药影响的研究 作者:Kristiana Xhima, Dallan McMahon, Edward Ntiri, Maged Goubran, Kullervo Hynynen and Isabelle Aubert日期:06/20/2021,浏览量:1433,Q&A: 1

Basal forebrain cholinergic neurons (BFCNs) regulate circuit dynamics underlying cognitive processing, including attention, memory, and cognitive flexibility. In Alzheimer’s disease and related neurodegenerative conditions, the degeneration of BFCNs has long been considered a key player in cognitive decline. The cholinergic system thus

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Dual Color, Live Imaging of Vesicular Transport in Axons of Cultured Sensory Neurons 培养感觉神经元轴突囊泡运输的双色实时成像 作者:Yoko Bekku and James L. Salzer日期:06/20/2021,浏览量:1080,Q&A: 0

The function of neurons in afferent reception, integration, and generation of electrical activity relies on their strikingly polarized organization, characterized by distinct membrane domains. These domains have different compositions resulting from a combination of selective targeting and retention of membrane proteins. In neurons, most proteins

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植物科学

Micrografting in Arabidopsis Using a Silicone Chip 利用硅芯片进行拟南芥微嫁接 作者:Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi日期:06/20/2021,浏览量:1591,Q&A: 1

The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including defense mechanisms and responses to surrounding environments such as soil and light conditions.

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Peptide-mediated Targeting of Nanoparticles with Chemical Cargoes to Chloroplasts in Arabidopsis Plants 多肽介导的化学物质纳米粒对拟南芥叶绿体的靶向作用 作者:Israel Santana, Peiguang Hu, Su-Ji Jeon, Chris Castillo, Hann Tu and Juan Pablo Giraldo日期:06/20/2021,浏览量:1208,Q&A: 0

Plant nanobiotechnology is a flourishing field that uses nanomaterials to study and engineer plant function. Applications of nanotechnology in plants have great potential as tools for improving crop yield, tolerance to disease and environmental stress, agrochemical delivery of pesticides and fertilizers, and genetic modification and transformation

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干细胞

CRISPR/Cas9-mediated Precise SNP Editing in Human iPSC Lines CRISPR/Cas9介导的人iPSC细胞系SNP精确编辑 作者:Hanwen Zhang and Siwei Zhang日期:06/20/2021,浏览量:1475,Q&A: 0

Human induced pluripotent stem cells (hiPSCs) have been extensively used in the fields of developmental biology and disease modeling. CRISPR/Cas9 gene editing in iPSC lines often has a low frequency, which hampers its application in precise allele editing of disease-associated single nucleotide polymorphisms (SNPs), especially those in the

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系统生物学

Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease 用于研究人类疾病中蛋白质N-糖基化失调的N-糖肽丰度和N-糖基化位点的差异分析 作者:Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin日期:06/20/2021,浏览量:1062,Q&A: 0

Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass spectrometry-based glycoproteomics technologies enabling large-scale N-glycoproteome profiling of

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