发育生物学

分类

    GeneWeld: Efficient Targeted Integration Directed by Short Homology in Zebrafish
    GeneWeld:斑马鱼短同源性诱导的高效靶向整合

    Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Multiple design strategies for zebrafish gene targeting have previously been reported with widely varying frequencies for

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    An Improved Method for Individual Tracking of Voluntary Wheel Running in Pair-housed Juvenile Mice
    一种对饲养的幼鼠自主跑轮运动个体跟踪的改进方法
    作者:  David A. Valientes, Anthony M. RausAutumn S. lvy, 日期: 07/05/2021, 浏览量: 681, Q&A: 0

    Rodent cages equipped with access to a voluntary running wheel are commonly used to study the effects of aerobic physical activity on physiology and behavior. Notable discoveries in exercise neurobiology, including the key role of brain-derived

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    A More Standardized Method for Imaging and Measuring Drosophila Thorax Length Using ImageJ
    一种使用ImageJ的更标准化的成像和测量果蝇的胸部长度的方法
    作者:  Emily K. Fowler, Stewart Leigh, Amanda BretmanTracey Chapman, 日期: 06/20/2021, 浏览量: 862, Q&A: 0

    Thorax measurements are important metrics of Drosophila fitness and are often correlated with key life-history traits, such as lifespan and fecundity. Therefore, thorax length is a widely used measure in studies of genetic variation and plasticity

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    Analysis of Caenorhabditis elegans Sperm Number, Size, Activation, and Mitochondrial Content
    秀丽隐杆线虫精子数量、大小、活化及线粒体含量分析
    作者:  Amy M. Hammerquist, Chia-An YenSean P. Curran, 日期: 06/05/2021, 浏览量: 1186, Q&A: 0

    Infertility is a widespread and often unexplained issue. Studying reproduction using C. elegans males offers insight into the influence of individual factors on male fertility in humans. We have created a collection of protocols to assess several

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    Rice Root Hair Phenotypes Imaged by Cryo-SEM
    水稻根毛表型的低温扫描电镜成像

    Cryo-scanning electron microscopy (cryo-SEM) was first introduced for scientific use in the 1980s. Since then, cryo-SEM has become a routine technique for studying the surfaces and internal structures of biological samples with a high water content.

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    Quantitation of Secretory Granule Size in Drosophila Larval Salivary Glands
    果蝇幼虫唾液腺分泌颗粒大小的定量研究
    作者:  Cheng-I J. MaJulie A. Brill, 日期: 06/05/2021, 浏览量: 1141, Q&A: 0

    Maturation of secretory granules is a crucial process that ensures the bioactivity of cargo proteins undergoing regulated secretion. In Drosophila melanogaster, the larval salivary glands produce secretory granules that are up to four-fold larger in

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    Visualization and Quantitation of Wg trafficking in the Drosophila Wing Imaginal Epithelium
    果蝇翅成虫上皮中Wg运输的可视化和定量研究
    作者:  Leonie Witte, Karen Linnemannstöns, Mona Honemann-CapitoJulia Christina Gross, 日期: 06/05/2021, 浏览量: 1185, Q&A: 0

    Secretory Wnt trafficking can be studied in the polarized epithelial monolayer of Drosophila wing imaginal discs (WID). In this tissue, Wg (Drosophila Wnt-I) is presented on the apical surface of its source cells before being internalized into the

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    Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis
    小鼠多能干细胞衍生干细胞样结构的生成:植入术后胚胎发生的体外模型

    Post-implantation mammalian embryogenesis involves profound molecular, cellular, and morphogenetic changes. The study of these highly dynamic processes is complicated by the limited accessibility of in utero development. In recent years, several

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    Isolation of First-Trimester and Full-term Human Placental Hofbauer Cells
    妊娠早期和足月人胎盘霍夫鲍尔细胞的分离
    作者:  Anna Appios, Jake R. ThomasNaomi McGovern, 日期: 06/05/2021, 浏览量: 1551, Q&A: 0

    The placenta is the crucial organ that regulates the health of both mother and fetus during pregnancy. The human placenta is composed of villous tree-like structures that embed into the maternal decidua. Within the stroma of the villi resides a

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    Intact in situ Preparation of Drosophila melanogaster Lymph Gland for Comprehensive Analysis of Larval Hematopoiesis
    原位完整制备黑腹果蝇血淋巴作幼虫造血功能综合分析
    作者:  Diana Rodrigues, K. VijayRaghavan, Lucas WaltzerManeesha S. Inamdar, 日期: 05/20/2021, 浏览量: 2435, Q&A: 0

    Blood cells have a limited lifespan and are replenished by a small number of hematopoietic stem and progenitor cells (HSPCs). Adult vertebrate hematopoiesis occurs in the bone marrow, liver and spleen making a comprehensive analysis of the entire

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