植物科学

分类

    Quality Control and Preprocessing of Sequencing Reads
    测序读数的质量控制和预处理
    作者:  Zhiqiang Hao, Xiaojuan LiangGuanglin Li, 日期: 07/05/2022, 浏览量: 60, Q&A: 0

    Quality control and preprocessing of sequences are essential before analyzing high-throughput sequence data. After raw read data is generated from high-through sequencing platforms, quality control and preprocessing of sequencing reads should

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    Differential Expression Analysis: Simple Pair, Interaction, Time-series
    微分表达式分析:简单对、交互、时间序列
    作者:  Han Qu, Meng Qu, Shibo Wang, Lei Yu, Qiong Jia, Xuesong WangZhenyu Jia, 日期: 07/05/2022, 浏览量: 76, Q&A: 0

    Identifying differentially expressed (DE) genes across specific conditions is vital in understanding phenotypic variation. The fast-growing RNA sequencing (RNA-seq) provides much information that efficiently quantifies gene expression. Methods and

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    Maize Genome Assembly with PacBio Reads
    利用PacBio Reads组装玉米基因组
    作者:  Ying HuMarcio F. R. Resende Jr., 日期: 07/05/2022, 浏览量: 26, Q&A: 0

    Assembly of high-quality genomes is critical for the characterization of structural variations (SV), for the development of a high-resolution map of polymorphisms, and to serve as the foundation for gene annotation. In recent years, the advent

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    GO/KEGG Enrichment Analysis on Gene Lists from Rice (Oryza Sativa)
    水稻基因列表GO/KEGG富集分析
    作者:  Yahui Li, 日期: 06/20/2022, 浏览量: 84, Q&A: 0

    In RNA-seq data analysis, functional enrichment analysis on genes has become a routine. Many enrichment analysis software and web-applications have emerged. However, gene annotation information is only easily accessible for the most well-studied

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    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    白葵花分生组织的定量实时共聚焦成像
    作者:  Ya Min, Stephanie J. ConwayElena M. Kramer, 日期: 06/20/2022, 浏览量: 1020, Q&A: 0

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis

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    Quantitative Analysis of Redox Pool (NAD+, NADH Content) in Plant Samples Under Aluminum Stress
    铝胁迫下植物样品氧化还原池(NAD+, NADH含量)的定量分析
    作者:  Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki KoyamaSanjib Kumar Panda, 日期: 06/20/2022, 浏览量: 989, Q&A: 0

    Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial

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    Identification of Expression QTL by QTLtools in a Rice Recombinant Inbred Line Population
    水稻重组自交系群体中利用QTLtools鉴定QTL的表达
    作者:  Feng Xiong, Hu ZhaoWeibo Xie, 日期: 06/20/2022, 浏览量: 127, Q&A: 0

    Expression QTL (eQTL) analysis assesses the association between the expression levels of target genes and genotypes of genetic markers to identify loci that regulate the expression of target genes. eQTL results can be used to construct genetic

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    An Analysis Pipeline for Identification of RNA Modification, Alternative Splicing and Polyadenylation Using Third Generation Sequencing
    使用第三代测序鉴定 RNA 修饰、可变剪接和多聚腺苷酸化的分析流程
    作者:  Yuxiang Liufu, Xuqing Liu, Lin Wu, Hangxiao Zhang, Yubang GaoLianfeng Gu, 日期: 06/05/2022, 浏览量: 243, Q&A: 0

    Nanopore sequencing based on Oxford Nanopore Technologies (ONT) and Pacific BioSciences (PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) have shown great potential in detecting post-transcriptional regulation. Direct

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    Investigation of Transposon DNA Methylation and Copy Number Variation in Plants Using Southern Hybridisation
    利用Southern杂交技术研究植物中转座子DNA甲基化和拷贝数变异的情况
    作者:  Vivek Hari Sundar G.P. V. Shivaprasad, 日期: 06/05/2022, 浏览量: 505, Q&A: 0

    Plant genomes are pronouncedly enriched in repeat elements such as transposons. These repeats are epigenetically regulated by DNA methylation. Whole genome high-depth sequencing after bisulfite treatment remains an expensive and laborious method to

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    In vitro Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions
    评估蛋白质-磷脂相互作用的特异性和亲和力的体外测定

    Protein–lipid interactions play important roles in many biological processes, including metabolism, signaling, and transport; however, computational and structural analyses often fail to predict such interactions, and determining which lipids

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