神经科学

分类

    Automated Quantification of Multiple Cell Types in Fluorescently Labeled Whole Mouse Brain Sections Using QuPath
    QuPath对荧光标记的整个小鼠大脑切片中的多种细胞类型进行自动定量
    作者:  Jo-Maree Courtney, Gary P. Morris, Elise M. Cleary, David W. HowellsBrad A. Sutherland, 日期: 07/05/2022, 浏览量: 150, Q&A: 0

    The quantification of labeled cells in tissue sections is crucial to the advancement of biological knowledge. Traditionally, this was a tedious process, requiring hours of careful manual counting in small portions of a larger tissue section. To

    ...
    Protocol to Study Spatial Subgoal Learning Using Escape Behavior in Mice
    利用逃逸行为研究小鼠空间子目标学习的方案
    作者:  Philip ShamashTiago Branco, 日期: 06/20/2022, 浏览量: 788, Q&A: 0

    Rodent spatial navigation is a key model system for studying mammalian cognition and its neural mechanisms. Of particular interest is how animals memorize the structure of their environments and compute multi-step routes to a goal. Previous work on

    ...
    Time-off-pick Assay to Measure Caenorhabditis elegans Motility
    时间-间隔法测定秀丽隐杆线虫运动能力
    作者:  Alyssa C. Walker, Rohan Bhargava, Amanda S. Brust, Ali A. OwjiDaniel M. Czyż, 日期: 06/20/2022, 浏览量: 937, Q&A: 0

    Caenorhabditis elegans is a simple metazoan that is often used as a model organism to study various human ailments with impaired motility phenotypes, including protein conformational diseases. Numerous motility assays that measure neuro-muscular

    ...
    Vestibular Organ Dissection and Whole-Mount Immunolabeling in Mouse
    小鼠前庭器官解剖和整体免疫标记
    作者:  Timothy S. BalmerLaurence O. Trussell, 日期: 05/20/2022, 浏览量: 435, Q&A: 0

    The vestibular sensory apparatus contained in the inner ear is a marvelous evolutionary adaptation for sensing movement in 3 dimensions and is essential for an animal’s sense of orientation in space, head movement, and balance. Damage to these

    ...
    Identification and Monitoring of Nucleotide Repeat Expansions Using Southern Blotting in Drosophila Models of C9orf72 Motor Neuron Disease and Frontotemporal Dementia
    应用Southern Blotting鉴定和监测C9orf72运动神经元疾病和额颞叶痴呆模型的核苷酸重复扩增
    作者:  Joanne L. Sharpe, Nikki S. HarperRyan J. H. West, 日期: 05/20/2022, 浏览量: 423, Q&A: 0

    Repeat expansion diseases, including fragile X syndrome, Huntington’s disease, and C9orf72-related motor neuron disease and frontotemporal dementia, are a group of disorders associated with polymorphic expansions of tandem repeat nucleotide

    ...
    Translating Ribosome Affinity Purification (TRAP) of Cell Type-specific mRNA from Mouse Brain Lysates
    小鼠脑裂解物中细胞类型特异性 mRNA 的核糖体翻译亲和纯化 (TRAP)
    作者:  Catherine L. Salussolia, Kellen D. WindenMustafa Sahin, 日期: 05/05/2022, 浏览量: 629, Q&A: 0

    Mammalian tissues are highly heterogenous and complex, posing a challenge in understanding the molecular mechanisms regulating protein expression within various tissues. Recent studies have shown that translation at the level of the ribosome is

    ...
    High Throughput Blood-brain Barrier Organoid Generation and Assessment of Receptor-Mediated Antibody Transcytosis
    高通量血脑屏障类器官的产生和受体介导的抗体转胞吞作用的评估
    作者:  Elena Kassianidou, Claire Simonneau, Alina GavrilovRoberto Villaseñor, 日期: 04/20/2022, 浏览量: 675, Q&A: 0

    Targeting receptor-mediated transcytosis (RMT) is a successful strategy for drug delivery of biologic agents across the blood-brain barrier (BBB). The recent development of human BBB organoid models is a major advancement to help characterize the

    ...
    Self-Paced Five-Choice Serial Reaction Time-Task for Mouse Behavioral Testing
    用于评估小鼠执行功能的自定进度五选择系列反应时间任务
    作者:  Julie Cunningham, Leah Dinah Sheppard, Eduardo ListikQin Wang, 日期: 04/20/2022, 浏览量: 327, Q&A: 0

    The five-choice serial reaction time-task (5-CSRTT) is a behavioral rodent test that can assess executive functioning. It may be employed to analyze distinctive aspects of murine models for a plethora of diseases, such as attention deficit

    ...
    Stimulus-induced Robust Narrow-band Gamma Oscillations in Human EEG Using Cartesian Gratings
    使用笛卡尔光栅在人类脑电图中刺激诱导的稳健窄带伽马振荡
    作者:  Dinavahi V. P. S. MurtySupratim Ray, 日期: 04/05/2022, 浏览量: 460, Q&A: 0

    Stimulus-induced narrow-band gamma oscillations (20–70 Hz) are induced in the visual areas of the brain when particular visual stimuli, such as bars, gratings, or full-screen hue, are shown to the subject. Such oscillations are modulated by

    ...
    Multiplexing Thermotaxis Behavior Measurement in Caenorhabditis elegans
    秀丽隐杆线虫的多重趋热行为测量
    作者:  Stephan Raiders, Mason KleinAakanksha Singhvi, 日期: 04/05/2022, 浏览量: 857, Q&A: 0

    Thermotaxis behaviors in C. elegans exhibit experience-dependent plasticity of thermal preference memory. This behavior can be assayed either at population level, on linear temperature gradients, or at the individual animal level, by radial

    ...