免疫学

分类

    Large-scale Isolation of Exosomes Derived from NK Cells for Anti-tumor Therapy
    大规模分离来自NK细胞的外泌体用于抗肿瘤治疗

    Exosomes are lipid bilayer–enclosed vesicles, actively secreted by cells, containing proteins, lipids, nucleic acids, and other substances with multiple biological functions after entering target cells. Exosomes derived from NK cells have been

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    Expression and Purification of scFv2H7-P18F3, a Bi-Modular Fusion Protein (BMFP) Targeting Human CD20
    靶向人CD20的双模块融合蛋白scFv2H7-P18F3的表达和纯化

    P18F3-based bi-modular fusion proteins (BMFPs), designed to re-direct pre-existing anti-Epstein-Barr virus (EBV) endogenous polyclonal antibodies towards defined target cells, demonstrated efficient biological activity in a mouse tumor model and

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    Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4+, and CD8+ T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung
    从小鼠淋巴器官、血管周围脂肪组织、肾脏和肺分离的γδ、CD4+和CD8+T细胞中的白细胞介素-17A和干扰素-γ产生的测定
    作者:  Kevin Comeau, Antoine Caillon, Pierre ParadisErnesto L. Schiffrin, 日期: 05/20/2023, 浏览量: 246, Q&A: 0

    T cells localized to the kidneys and vasculature/perivascular adipose tissue (PVAT) play an important role in hypertension and vascular injury. CD4+, CD8+, and γδ T-cell subtypes are programmed to produce interleukin (IL)-17 or

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    Novel Antibody-independent Method to Measure Complement Deposition on Bacteria
    测量细菌补体沉积的独立于抗体的新方法
    作者:  Toska Wonfor, Shuxian LiMaisem Laabei, 日期: 05/05/2023, 浏览量: 215, Q&A: 0

    During infection, complement plays a critical role in inflammation, opsonisation, and destruction of microorganisms. This presents a challenge for pathogens such as Staphylococcus aureus to overcome when invading the host. Our current knowledge on

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    Anti-tumor Efficacy of CD19 CAR-T in a Raji B Cell Xenografted Mouse Model
    CD19 CAR-T在Raji B细胞异位移植小鼠模型中的抗肿瘤效果
    作者:  Qian XiaoXiaolei Su, 日期: 04/20/2023, 浏览量: 554, Q&A: 0

    Chimeric antigen receptor (CAR)-T therapy launched a new era for cancer treatments, displaying outstanding effectiveness in relapsed or refractory B-cell malignancies. Demonstrating the tumor-killing ability of CAR-Ts in mouse xenograft models

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    In situ Microinflammation Detection Using Gold Nanoclusters and a Tissue-clearing Method
    利用金纳米团簇和组织清除方法进行原位微炎症检测

    Microinflammation enhances the permeability of specific blood vessel sites through an elevation of local inflammatory mediators, such as interleukin (IL)-6 and tumor necrosis factor (TNF)-α. By a two-dimensional immunohistochemistry analysis

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    Isolation of RNA from the Murine Colonic Tissue and qRT-PCR for Inflammatory Cytokines
    从小鼠结肠组织中分离 RNA 并采用qRT -PCR 检测炎症细胞因子
    作者:  Ibrahim M. Sayed, Kaili Inouye, Soumita DasLaura Crotty Alexander, 日期: 03/20/2023, 浏览量: 786, Q&A: 0

    E-cigarette (E-cig) inhalation affects health status by modulating inflammation profiles in several organs, including the brain, lung, heart, and colon. The effect of flavored fourth-generation pod-based E-cigs (JUUL) on murine gut inflammation ...

    A Tumor-admixture Model to Interrogate Immune Cell–dependent Tumorigenesis
    研究免疫细胞依赖性肿瘤发生的肿瘤混合模型
    作者:  Jordan T. Noe, Chuanlin Ding, Anne E. Geller, Beatriz E. Rendon, Jun YanRobert A. Mitchell, 日期: 03/05/2023, 浏览量: 418, Q&A: 0

    A rigorous determination of effector contributions of tumor-infiltrating immune cells is critical for identifying targetable molecular mechanisms for the development of novel cancer immunotherapies. A tumor/immune cell–admixture model is an

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    CRISPR/Cas9-based Engineering of Immunoglobulin Loci in Hybridoma Cells
    杂交瘤细胞中基于 CRISPR/Cas9 的免疫球蛋白位点工程

    Development of the hybridoma technology by Köhler and Milstein (1975) has revolutionized the immunological field by enabling routine use of monoclonal antibodies (mAbs) in research and development efforts, resulting in their successful

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    Continuous Measurement of Reactive Oxygen Species Formation in Bacteria-infected Bone Marrow–derived Macrophages Using a Fluorescence Plate Reader
    使用荧光酶标仪连续测量受细菌感染的骨髓源性巨噬细胞中活性氧的生成

    Macrophages are at the center of innate immunity and are the main target cells of the intracellular pathogen Salmonella enterica serovar Typhi. The production of reactive oxygen and nitrogen species (ROS/RNS) is the host’s early response to

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