微生物学

分类

    VirScan: High-throughput Profiling of Antiviral Antibody Epitopes
    Vir Scan: 抗病毒抗体表位的高通量分析
    作者:  Ellen L. Shrock, Christine L. ShrockStephen J. Elledge, 日期: 07/05/2022, 浏览量: 96, Q&A: 0

    Profiling the specificities of antibodies can reveal a wealth of information about humoral immune responses and the antigens they target. Here, we present a protocol for VirScan, an application of the phage immunoprecipitation sequencing (PhIP-Seq)

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    A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differences in Fitness during Plant Infection
    基于β-葡萄糖醛酸酶 (GUS) 的细菌竞争测定以评估植物感染期间适应性的细微差异
    作者:  Julien S. Luneau, Laurent D. Noël, Emmanuelle LauberAlice Boulanger, 日期: 07/05/2022, 浏览量: 47, Q&A: 0

    Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight

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    A Modified Fluctuation Assay with a CAN1 Reporter in Yeast
    酵母中CAN1报告基因的改良波动测定
    作者:  Pengyao Jiang, Anja R. OllodartMaitreya J. Dunham, 日期: 06/05/2022, 浏览量: 528, Q&A: 0

    Understanding the generation of mutations is fundamental to understanding evolution and genetic disease; however, the rarity of such events makes experimentally identifying them difficult. Mutation accumulation (MA) methods have been widely used. MA

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    Flow Cytometry-based Measurement of Reactive Oxygen Species in Cyanobacteria
    基于流式细胞仪的蓝藻活性氧测量
    作者:  Soumila MondalShailendra P. Singh, 日期: 05/20/2022, 浏览量: 649, Q&A: 0

    Cyanobacteria are Gram-negative oxygen-producing photosynthetic bacteria that are useful in the pharmaceutical and biofuel industries. Monitoring of oxidative stress under fluctuating environmental conditions is important for determining the

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    Purification of the Bacterial Amyloid “Curli” from Salmonella enterica Serovar Typhimurium and Detection of Curli from Infected Host Tissues
    从沙门氏菌鼠伤寒血清中纯化细菌淀粉样蛋白“Curli”并检测受感染宿主组织中的 Curli

    Microbiologists are learning to appreciate the importance of “functional amyloids” that are produced by numerous bacterial species and have impacts beyond the microbial world. These structures are used by bacteria to link together,

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    A Molecular Cloning and Sanger Sequencing-based Protocol for Detecting Site-specific DNA Methylation
    一种基于分子克隆和Sanger测序的位点特异性DNA甲基化检测方案
    作者:  Wei Guo, Anthony CannonDamon Lisch, 日期: 05/05/2022, 浏览量: 696, Q&A: 1

    DNA methylation is a conserved chemical modification, by which methyl groups are added to the cytosine of DNA molecules. Methylation can influence gene expression without changing the sequence of a particular gene. This epigenetic effect is an

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    Expression, Purification, and in vitro Enzyme Activity Assay of a Recombinant Aldehyde Dehydrogenase from Thermus thermophilus, using an Escherichia coli host
    使用大肠杆菌宿主的嗜热栖热菌重组醛脱氢酶的表达、纯化和体外酶活性测定
    作者:  Kim Shortall, Edmond MagnerTewfik Soulimane, 日期: 05/05/2022, 浏览量: 759, Q&A: 0

    Based on previous in-depth characterisation, aldehyde dehydrogenases (ALDH) are a diverse superfamily of enzymes, in terms of both structure and function, present in all kingdoms of life. They catalyse the oxidation of an aldehyde to carboxylic acid

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    Ex vivo Human Skin Infection with Herpes Simplex Virus 1
    人皮肤单纯疱疹病毒1的体外感染
    作者:  Nydia C. De La Cruz, Maureen Möckel, Lisa WirtzDagmar Knebel-Mörsdorf, 日期: 05/05/2022, 浏览量: 484, Q&A: 0

    Although herpes simplex virus 1 (HSV-1) is a well-studied virus, how the virus invades its human host via skin and mucosa to reach its receptors and initiate infection remains an open question. For studies of HSV-1 infection in skin, mice have been

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    Bacterial Growth Curve Measurements with a Multimode Microplate Reader
    使用多模式酶标仪测量细菌生长曲线
    作者:  Ariel T. Rogers, Kaitlin R. Bullard, Akash C. DodYong Wang, 日期: 05/05/2022, 浏览量: 1020, Q&A: 0

    Bacterial studies based on growth curves are common in microbiology and related fields. Compared to the standard photometer and cuvette based protocols, bacterial growth curve measurements with microplate readers provide better temporal resolution,

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    Method for Analysing Protein Structure of SARS-COV-2 M Protein for Possible Clues Regarding Virion Stability, Longevity and Transmission
    通过分析SARS-COV-2 M蛋白结构为确定病毒稳定性、存活时间和传播提供可能线索
    作者:  Saam HasanMuhammad Maqsud Hossain, 日期: 05/05/2022, 浏览量: 170, Q&A: 0

    The Severe Acute Respiratory Syndrome Coronavirus 2 or SARS-CoV-2 has been the causative agent behind the 2020 global pandemic. With the numbers infected rising well above a 2.7 million and confirmed deaths above 122,000 as of 25th April 2020, it

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