生物化学

分类

    Whole-mount Senescence-Associated Beta-Galactosidase (SA-β-GAL) Activity Detection Protocol for Adult Zebrafish
    成年斑马鱼的整体衰老相关 β-半乳糖苷酶 (SA-β-GAL) 活性检测方案
    作者:  Marta Marzullo, Mounir El MaïMiguel Godinho Ferreira, 日期: 07/05/2022, 浏览量: 80, Q&A: 0

    Senescence-associated beta-galactosidase (SA-β-GAL) is an enzyme that accumulates in the lysosomes of senescent cells, where it hydrolyses β-galactosides. With p16, it represents a well-recognized biomarker used to assess senescence both

    ...
    Quality Control and Preprocessing of Sequencing Reads
    测序读数的质量控制和预处理
    作者:  Zhiqiang Hao, Xiaojuan LiangGuanglin Li, 日期: 07/05/2022, 浏览量: 60, Q&A: 0

    Quality control and preprocessing of sequences are essential before analyzing high-throughput sequence data. After raw read data is generated from high-through sequencing platforms, quality control and preprocessing of sequencing reads should

    ...
    Quantitative Analysis of Redox Pool (NAD+, NADH Content) in Plant Samples Under Aluminum Stress
    铝胁迫下植物样品氧化还原池(NAD+, NADH含量)的定量分析
    作者:  Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki KoyamaSanjib Kumar Panda, 日期: 06/20/2022, 浏览量: 989, Q&A: 0

    Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial

    ...
    Patterned Substrate of Mobile and Immobile Ligands to Probe EphA2 Receptor Clustering
    用于探测 EphA2 受体聚类的移动和固定配体的图案化底物
    作者:  Zhongwen Chen, Kabir H. BiswasJay T. Groves, 日期: 06/05/2022, 浏览量: 783, Q&A: 0

    A multitude of membrane-localized receptors are utilized by cells to integrate both biochemical and physical signals from their microenvironment. The clustering of membrane receptors is widely presumed to have functional consequences for subsequent

    ...
    H2O2 Release Assay
    H2O2释放测定
    作者:  Santosh Yadav, Shruthi Sanjitha Sampath, Brian J. DeskinVictor J. Thannickal, 日期: 06/05/2022, 浏览量: 1117, Q&A: 0

    Reactive oxygen species are ubiquitous in nature, and function as signalling molecules in biological systems; they may also contribute to oxidative stress in several pathobiological disease states. In this report, we describe a simple, reliable,

    ...
    Click-iT® Plus OPP Alexa Fluor® Protein Synthesis Assay in Embryonic Cells
    在胚胎细胞中Click- iT®Plus OPP Alexa Fluor®蛋白合成分析
    作者:  Yan Li, Xu Ji, Lu Chang, Jianan Tang, Min-Min Hua, Jing Liu, Christopher O’Neil, Xuefeng HuangXingliang Jin, 日期: 06/05/2022, 浏览量: 611, Q&A: 0

    This protocol describes a method to assess relative changes in the level of global protein synthesis in the preimplantation embryo using the Click-iT® Plus OPP Protein Synthesis Assays. In this assay, O-propargyl-puromycin (OPP), an analog of

    ...
    Investigation of Transposon DNA Methylation and Copy Number Variation in Plants Using Southern Hybridisation
    利用Southern杂交技术研究植物中转座子DNA甲基化和拷贝数变异的情况
    作者:  Vivek Hari Sundar G.P. V. Shivaprasad, 日期: 06/05/2022, 浏览量: 505, Q&A: 0

    Plant genomes are pronouncedly enriched in repeat elements such as transposons. These repeats are epigenetically regulated by DNA methylation. Whole genome high-depth sequencing after bisulfite treatment remains an expensive and laborious method to

    ...
    In vitro Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions
    评估蛋白质-磷脂相互作用的特异性和亲和力的体外测定

    Protein–lipid interactions play important roles in many biological processes, including metabolism, signaling, and transport; however, computational and structural analyses often fail to predict such interactions, and determining which lipids

    ...
    Preparation of Cas9 Ribonucleoproteins for Genome Editing
    用于基因组编辑的 Cas9 核糖核蛋白的制备
    作者:  Sheng-Wei Lin, Viet Quoc NguyenSteven Lin, 日期: 05/20/2022, 浏览量: 1349, Q&A: 0

    Genome editing by the delivery of pre-assembled Cas9 ribonucleoproteins (Cas9 RNP) is an increasingly popular approach for cell types that are difficult to manipulate genetically by the conventional plasmid and viral methods. Cas9 RNP editing is

    ...
    Identification and Monitoring of Nucleotide Repeat Expansions Using Southern Blotting in Drosophila Models of C9orf72 Motor Neuron Disease and Frontotemporal Dementia
    应用Southern Blotting鉴定和监测C9orf72运动神经元疾病和额颞叶痴呆模型的核苷酸重复扩增
    作者:  Joanne L. Sharpe, Nikki S. HarperRyan J. H. West, 日期: 05/20/2022, 浏览量: 423, Q&A: 0

    Repeat expansion diseases, including fragile X syndrome, Huntington’s disease, and C9orf72-related motor neuron disease and frontotemporal dementia, are a group of disorders associated with polymorphic expansions of tandem repeat nucleotide

    ...