生物化学

分类

    Cycloheximide (CHX) Chase Assay to Examine Protein Half-life
    环己酰亚胺(CHX)追踪测定法检测蛋白质半衰期
    作者:  Ying Miao, Qian Du, Hong-Guang Zhang, Yukang Yuan, Yibo ZuoHui Zheng, 日期: 06/05/2023, 浏览量: 59, Q&A: 0

    Cycloheximide (CHX) is a small molecule derived from Streptomyces griseus that acts as fungicide. As a ribosome inhibitor, CHX can restrict the translation elongation of eukaryotic protein synthesis. Once protein synthesis is inhibited by CHX, the

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    Measurement of Total Phosphorus and Polyphosphate in Chlamydomonas reinhardtii
    莱茵衣藻中总磷和多磷酸盐的测定
    作者:  Yujie Yang, Suna Ren, Xianqing Jia, Houqing Zeng, Long Wang, Yiyong ZhuKeke Yi, 日期: 06/05/2023, 浏览量: 38, Q&A: 0

    Phosphorus is an essential nutrient for plants. Green algae usually store excess P as polyphosphate (polyP) in the vacuoles. PolyP, a linear chain of three to hundreds of phosphate residues linked by phosphoanhydride bonds, is important for cell

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    Revised iCLIP-seq Protocol for Profiling RNA–protein Interaction Sites at Individual Nucleotide Resolution in Living Cells
    用于在活细胞中以单个核苷酸分辨率分析RNA-蛋白质相互作用位点的修订iCLIP-seq方案
    作者:  Syed Nabeel-ShahJack F. Greenblatt, 日期: 06/05/2023, 浏览量: 159, Q&A: 0

    Individual nucleotide resolution UV cross-linking and immunoprecipitation followed by high-throughput sequencing (iCLIP-seq) is a powerful technique that is used to identify RNA-binding proteins’ (RBP) binding sites on target RNAs and to

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    Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors
    脂质共轭荧光pH传感器的合成与纯化
    作者:  Wiebke Wiesner, Ronja Marie KühnelThomas Guenther Pomorski, 日期: 06/05/2023, 浏览量: 51, Q&A: 0

    Lipid-conjugated pH sensors based on fluorophores coupled to lipids are a powerful tool for monitoring pH gradients in biological microcompartments and reconstituted membrane systems. This protocol describes the synthesis of pH sensors based on

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    Expression and Purification of scFv2H7-P18F3, a Bi-Modular Fusion Protein (BMFP) Targeting Human CD20
    靶向人CD20的双模块融合蛋白scFv2H7-P18F3的表达和纯化

    P18F3-based bi-modular fusion proteins (BMFPs), designed to re-direct pre-existing anti-Epstein-Barr virus (EBV) endogenous polyclonal antibodies towards defined target cells, demonstrated efficient biological activity in a mouse tumor model and

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    Measuring in vitro ATPase Activity with High Sensitivity Using Radiolabeled ATP
    用放射性标记的ATP高灵敏度测定体外ATP酶活性
    作者:  Sarina VeitThomas Günther Pomorski, 日期: 05/20/2023, 浏览量: 255, Q&A: 0

    ATPase assays are a common tool for the characterization of purified ATPases. Here, we describe a radioactive [γ-32P]-ATP-based approach, utilizing complex formation with molybdate for phase separation of the free phosphate from

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    Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4+, and CD8+ T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung
    从小鼠淋巴器官、血管周围脂肪组织、肾脏和肺分离的γδ、CD4+和CD8+T细胞中的白细胞介素-17A和干扰素-γ产生的测定
    作者:  Kevin Comeau, Antoine Caillon, Pierre ParadisErnesto L. Schiffrin, 日期: 05/20/2023, 浏览量: 246, Q&A: 0

    T cells localized to the kidneys and vasculature/perivascular adipose tissue (PVAT) play an important role in hypertension and vascular injury. CD4+, CD8+, and γδ T-cell subtypes are programmed to produce interleukin (IL)-17 or

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    A Simple, Reproducible Procedure for Chemiluminescent Western Blot Quantification
    一种简单、可重复的化学发光蛋白质印迹定量方法
    作者:  Daniel Cañada-GarcíaJuan C. Arévalo, 日期: 05/05/2023, 浏览量: 751, Q&A: 0

    Western blotting is a universally used technique to identify specific proteins from a heterogeneous and complex mixture. However, there is no clear and common procedure to quantify the results obtained, resulting in variations due to the

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    A Modified Acyl-RAC Method of Isolating Retinal Palmitoyl Proteome for Subsequent Detection through LC-MS/MS
    一种改良的酰基-RAC方法分离视网膜棕榈酰蛋白质组,并通过LC-MS/MS进行后续检测

    Palmitoylation is a unique and reversible posttranslational lipid modification (PTM) that plays a critical role in many cellular events, including protein stability, activity, membrane association, and protein–protein interactions. The dynamic

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    In vitro Reconstitution of Phase-separated p62 Bodies on the Arp2/3-derived Actin Network
    相分离的p62体在Arp2/3衍生的肌动蛋白网络上的体外重组
    作者:  Tong Liu, Mengbo XuNa Mi, 日期: 04/20/2023, 浏览量: 335, Q&A: 0

    In cells, p62/SQSTM1 undergoes liquid–liquid phase separation (LLPS) with poly-ubiquitin chains to form p62 bodies that work as a hub for various cellular events, including selective autophagy. Cytoskeleton components such as Arp2/3-derived

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