细胞生物学

分类

    Cycloheximide (CHX) Chase Assay to Examine Protein Half-life
    环己酰亚胺(CHX)追踪测定法检测蛋白质半衰期
    作者:  Ying Miao, Qian Du, Hong-Guang Zhang, Yukang Yuan, Yibo ZuoHui Zheng, 日期: 06/05/2023, 浏览量: 59, Q&A: 0

    Cycloheximide (CHX) is a small molecule derived from Streptomyces griseus that acts as fungicide. As a ribosome inhibitor, CHX can restrict the translation elongation of eukaryotic protein synthesis. Once protein synthesis is inhibited by CHX, the

    ...
    Quantifying Single and Dual Channel Live Imaging Data: Kymograph Analysis of Organelle Motility in Neurons
    量化单通道和双通道实时成像数据: 神经元中细胞器运动的Kymograph分析
    作者:  Laura Digilio, Lloyd P. McMahon, Alois Duston, Chan Choo YapBettina Winckler, 日期: 05/20/2023, 浏览量: 440, Q&A: 0

    Live imaging is commonly used to study dynamic processes in cells. Many labs carrying out live imaging in neurons use kymographs as a tool. Kymographs display time-dependent microscope data (time-lapsed images) in two-dimensional representations

    ...
    THRIFTY—A High-throughput Single Muscle Fiber Typing Method Based on Immunofluorescence Detection
    THRIFTY-一种基于免疫荧光检测的高通量单肌纤维分型方法
    作者:  Sebastian Edman, Oscar HorwathWilliam Apró, 日期: 05/20/2023, 浏览量: 265, Q&A: 0

    Skeletal muscle consists of a mixture of fiber types with different functional and metabolic characteristics. The relative composition of these muscle fiber types has implications for muscle performance, whole-body metabolism, and health. However,

    ...
    In vivo Drug Screening to Identify Anti-metastatic Drugs in Twist1a-ERT2 Transgenic Zebrafish
    Twist1a-ERT2转基因斑马鱼中进行体内药物筛选以确定抗转移药物
    作者:  Joji Nakayama, Hideki MakinoshimaZhiyuan Gong, 日期: 05/20/2023, 浏览量: 244, Q&A: 0

    Here, we present an in vivo drug screening protocol using a zebrafish model of metastasis for the identification of anti-metastatic drugs. A tamoxifen-controllable Twist1a-ERT2 transgenic zebrafish line was established to serve as a

    ...
    Implementation of a Drug Screening Platform to Target Gch1 Expression in Injured Mouse Dorsal Root Ganglion Neurons
    在受损小鼠背根神经节神经元中靶向Gch1表达的药物筛选平台的实现

    Management of neuropathic pain is notoriously difficult; current analgesics, including anti-inflammatory- and opioid-based medications, are generally ineffective and can pose serious side effects. There is a need to uncover non-addictive and safe

    ...
    Simultaneous Microendoscopic Calcium Imaging and EEG Recording of Mouse Brain during Sleep
    小鼠睡眠期间大脑的同时显微内窥镜钙成像和脑电图记录
    作者:  Sasa TengYueqing Peng, 日期: 05/05/2023, 浏览量: 176, Q&A: 0

    Sleep is a conserved biological process in the animal kingdom. Understanding the neural mechanisms underlying sleep state transitions is a fundamental goal of neurobiology, important for the development of new treatments for insomnia and other

    ...
    E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
    使用Bruker Skyscan 1172显微CT对E15.5小鼠胚胎进行显微CT检查
    作者:  Elena Astanina, Sara Petrillo, Tullio Genova, Federico MussanoFederico Bussolino, 日期: 05/05/2023, 浏览量: 178, Q&A: 0

    X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology

    ...
    Modified Pseudo-Schiff Propidium Iodide for Staining the Shoot Apical Meristem in Arabidopsis
    改良的拟Schiff碘化丙啶对拟南芥茎尖分生组织的染色
    作者:  Ruiqi Li, Qi LiLigeng Ma, 日期: 05/05/2023, 浏览量: 315, Q&A: 0

    Visualization of cell structure with fluorescent dye for characterizing cell size, shape, and arrangement is a common method to study tissue morphology and morphogenesis. In order to observe shoot apical meristem (SAM) in Arabidopsis thaliana by

    ...
    Assessment of Chemosensory Response to Volatile Compounds in Healthy, Aged, and Neurodegenerative Caenorhabditis elegans Models
    评估健康、老年和神经退行性秀丽隐杆线虫模型对挥发性化合物的化学感觉反应
    作者:  Cira CrespoRoberto Grau, 日期: 05/05/2023, 浏览量: 195, Q&A: 0

    A basic function of the nervous system is to confer the ability to detect external stimuli and generate appropriate behavioral and physiological responses. These can be modulated when parallel streams of information are provided to the nervous

    ...