细胞生物学

分类

    A Robust Nanoparticle-based Magnetic Separation Method for Intact Lysosomes
    一种用于分离完整溶酶体的基于纳米颗粒的磁分离方法
    作者:  The Son Le, Mari TakahashiShinya Maenosono, 日期: 07/05/2022, 浏览量: 109, Q&A: 0

    Lysosome isolation is a preresiquite for identifying lysosomal protein composition by mass spectroscopic analysis, to reveal lysosome functions, and their involvement in some diseases. Magnetic nanoparticle-based fractionation has received great

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    A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differences in Fitness during Plant Infection
    基于β-葡萄糖醛酸酶 (GUS) 的细菌竞争测定以评估植物感染期间适应性的细微差异
    作者:  Julien S. Luneau, Laurent D. Noël, Emmanuelle LauberAlice Boulanger, 日期: 07/05/2022, 浏览量: 47, Q&A: 0

    Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight

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    Whole-mount Senescence-Associated Beta-Galactosidase (SA-β-GAL) Activity Detection Protocol for Adult Zebrafish
    成年斑马鱼的整体衰老相关 β-半乳糖苷酶 (SA-β-GAL) 活性检测方案
    作者:  Marta Marzullo, Mounir El MaïMiguel Godinho Ferreira, 日期: 07/05/2022, 浏览量: 80, Q&A: 0

    Senescence-associated beta-galactosidase (SA-β-GAL) is an enzyme that accumulates in the lysosomes of senescent cells, where it hydrolyses β-galactosides. With p16, it represents a well-recognized biomarker used to assess senescence both

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    In vivo Characterization of Endogenous Protein Interactomes in Drosophila Larval Brain, Using a CRISPR/Cas9-based Strategy and BioID-based Proximity Labeling
    利用CRISPR/Cas9 策略和基于BioID的邻近标记研究果蝇幼虫大脑内源性蛋白相互作用体的体内特性
    作者:  Ezgi Uçkun, Georg Wolfstetter, Johannes FuchsRuth H. Palmer, 日期: 07/05/2022, 浏览量: 65, Q&A: 0

    Understanding protein-protein interactions (PPIs) and interactome networks is essential to reveal molecular mechanisms mediating various cellular processes. The most common method to study PPIs in vivo is affinity purification combined with mass

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    Automated Quantification of Multiple Cell Types in Fluorescently Labeled Whole Mouse Brain Sections Using QuPath
    QuPath对荧光标记的整个小鼠大脑切片中的多种细胞类型进行自动定量
    作者:  Jo-Maree Courtney, Gary P. Morris, Elise M. Cleary, David W. HowellsBrad A. Sutherland, 日期: 07/05/2022, 浏览量: 150, Q&A: 0

    The quantification of labeled cells in tissue sections is crucial to the advancement of biological knowledge. Traditionally, this was a tedious process, requiring hours of careful manual counting in small portions of a larger tissue section. To

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    Experimental Models for Cold Exposure of Muscle in vitro and in vivo
    体外和体内肌肉低温暴露实验模型
    作者:  Tiril Schjølberg, Lucia Asoawe, Solveig Krapf, Arild C. Rustan, G. Hege ThoresenFred Haugen, 日期: 07/05/2022, 浏览量: 52, Q&A: 0

    Work in cold environments may have a significant impact on occupational health. In these and similar situations, cold exposure localized to the extremities may reduce the temperature of underlying tissues. To investigate the molecular effects of

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    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    白葵花分生组织的定量实时共聚焦成像
    作者:  Ya Min, Stephanie J. ConwayElena M. Kramer, 日期: 06/20/2022, 浏览量: 1020, Q&A: 0

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis

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    Fluorescent Labeling of Small Extracellular Vesicles (EVs) Isolated from Conditioned Media
    从条件培养基中分离的细胞外小泡的荧光标记
    作者:  John Santelices, Mark Ou, Winnie W. Hui, Gustavo H. B. MaegawaMariola J. Edelmann, 日期: 06/20/2022, 浏览量: 1597, Q&A: 0

    Extracellular vesicles (EVs), such as exosomes, are produced by all known eukaryotic cells, and constitute essential means of intercellular communication. Recent studies have unraveled the important roles of EVs in migrating to specific sites and

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    Labelling of Active Transcription Sites with Argonaute NRDE-3—Image Active Transcription Sites in vivo in Caenorhabditis elegans
    用Argonaute NRDE-3标记活性转录位点—图像秀丽隐杆线虫体内的活性转录位点
    作者:  Antoine BarrièreVincent Bertrand, 日期: 06/05/2022, 浏览量: 522, Q&A: 0

    Live labelling of active transcription sites is critical to our understanding of transcriptional dynamics. In the most widely used method, RNA sequence MS2 repeats are added to the transcript of interest, on which fluorescently tagged Major Coat

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    Electroporation of Small Interfering RNAs into Tibialis Anterior Muscles of Mice
    将小干扰 RNA 电穿孔到小鼠胫骨前肌中
    作者:  Anna Stephan, Flavia A. Graca, Liam C. HuntFabio Demontis, 日期: 06/05/2022, 浏览量: 795, Q&A: 0

    Aging and wasting of skeletal muscle reduce organismal fitness. Regrettably, only limited interventions are currently available to address this unmet medical need. Many methods have been developed to study this condition, including the

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