分子生物学

分类

    VirScan: High-throughput Profiling of Antiviral Antibody Epitopes
    Vir Scan: 抗病毒抗体表位的高通量分析
    作者:  Ellen L. Shrock, Christine L. ShrockStephen J. Elledge, 日期: 07/05/2022, 浏览量: 96, Q&A: 0

    Profiling the specificities of antibodies can reveal a wealth of information about humoral immune responses and the antigens they target. Here, we present a protocol for VirScan, an application of the phage immunoprecipitation sequencing (PhIP-Seq)

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    HaloChIP-seq for Antibody-Independent Mapping of Mouse Transcription Factor Cistromes in vivo
    HaloChIP-seq用于体内小鼠转录因子 Cistromes 的抗体独立映射

    Chromatin immunoprecipitation (ChIP) maps, on a genome-wide scale, transcription factor binding sites, and the distribution of other chromatin-associated proteins and their modifications. As such, it provides valuable insights into mechanisms of

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    Experimental Models for Cold Exposure of Muscle in vitro and in vivo
    体外和体内肌肉低温暴露实验模型
    作者:  Tiril Schjølberg, Lucia Asoawe, Solveig Krapf, Arild C. Rustan, G. Hege ThoresenFred Haugen, 日期: 07/05/2022, 浏览量: 52, Q&A: 0

    Work in cold environments may have a significant impact on occupational health. In these and similar situations, cold exposure localized to the extremities may reduce the temperature of underlying tissues. To investigate the molecular effects of

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    ATAC-Seq of a Single Myofiber from Mus musculus
    小家鼠单一肌纤维的ATAC-Seq
    作者:  Korin Sahinyan, Darren M. BlackburnVahab D. Soleimani, 日期: 06/20/2022, 浏览量: 1257, Q&A: 0

    Chromatin accessibility is a key determinant of gene expression that can be altered under different physiological and disease conditions. Skeletal muscle is made up of myofibers that are highly plastic and adaptive. Therefore, assessing the

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    A Modified Fluctuation Assay with a CAN1 Reporter in Yeast
    酵母中CAN1报告基因的改良波动测定
    作者:  Pengyao Jiang, Anja R. OllodartMaitreya J. Dunham, 日期: 06/05/2022, 浏览量: 528, Q&A: 0

    Understanding the generation of mutations is fundamental to understanding evolution and genetic disease; however, the rarity of such events makes experimentally identifying them difficult. Mutation accumulation (MA) methods have been widely used. MA

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    A Highly Sensitive Method to Efficiently Profile the Histone Modifications of FFPE Samples
    一种高效分析 FFPE 样品组蛋白修饰的高灵敏度方法
    作者:  Linxuan Zhao, Vamsi Krishna Polavarapu, Ram Prakash Yadav, Pengwei XingXingqi Chen, 日期: 05/20/2022, 浏览量: 768, Q&A: 0

    The majority of biopsies in both basic research and translational cancer studies are preserved in the format of archived formalin-fixed paraffin-embedded (FFPE) samples. Profiling histone modifications in archived FFPE tissues is critically

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    Purification of Mitochondrial Ribosomal Complexes from Trypanosoma cruzi and Leishmania tarentolae for Cryo-EM Analysis
    克氏锥虫和塔氏利什曼原虫线粒体核糖体复合物的纯化及低温电子显微镜分析
    作者:  Stéphanie Durrieu-Gaillard, Marie SisslerYaser Hashem, 日期: 05/20/2022, 浏览量: 920, Q&A: 1

    Kinetoplastids are unicellular eukaryotic parasites responsible for human pathologies such as Chagas disease, sleeping sickness or Leishmaniasis, caused by Trypanosoma cruzi, Trypanosoma brucei, and various Leishmania spp., respectively. They harbor

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    A Molecular Cloning and Sanger Sequencing-based Protocol for Detecting Site-specific DNA Methylation
    一种基于分子克隆和Sanger测序的位点特异性DNA甲基化检测方案
    作者:  Wei Guo, Anthony CannonDamon Lisch, 日期: 05/05/2022, 浏览量: 696, Q&A: 1

    DNA methylation is a conserved chemical modification, by which methyl groups are added to the cytosine of DNA molecules. Methylation can influence gene expression without changing the sequence of a particular gene. This epigenetic effect is an

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    An Improved EMSA-based Method to Prioritize Candidate cis-REs for Further Functional Validation
    一种改进的基于EMSA的方法来优先考虑候选的顺式RE,以进一步验证其功能
    作者:  Ting WuGang Li, 日期: 04/20/2022, 浏览量: 590, Q&A: 0

    Cells are the complex product of gene expression programs that involve the coordinated transcription of thousands of genes controlled by cis-regulatory elements (cis-REs). Therefore, identification of cis-REs is the key to decipher the mechanisms

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    circFL-seq, A Full-length circRNA Sequencing Method
    circFL-seq,一种全长circRNA的测序方法
    作者:  Zelin LiuEnce Yang, 日期: 04/20/2022, 浏览量: 988, Q&A: 1

    Due to overlapping sequences with linear cognates, identifying internal sequences of circular RNA (circRNA) remains a challenge. Recently, we have developed a full-length circRNA sequencing method (circFL-seq) and computational pipeline, to profile

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