系统生物学

分类

    Computational Analysis of Plasma Lipidomics from Mice Fed Standard Chow and Ketogenic Diet
    标准饲料和生酮饮食小鼠血浆脂质组学的计算分析
    作者:  Amy L. Seufert, James W. Hickman, Jaewoo ChoiBrooke A. Napier, 日期: 09/20/2023, 浏览量: 551, Q&A: 0

    Dietary saturated fatty acids (SFAs) are upregulated in the blood circulation following digestion. A variety of circulating lipid species have been implicated in metabolic and inflammatory diseases; however, due to the extreme variability in serum

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    Controlled Level of Contamination Coupled to Deep Sequencing (CoLoC-seq) Probes the Global Localisation Topology of Organelle Transcriptomes
    控制污染水平与深度测序 (CoLoC-seq) 相结合以探索细胞器转录组的全局定位拓扑
    作者:  Anna Smirnova, Damien JeandardAlexandre Smirnov, 日期: 09/20/2023, 浏览量: 208, Q&A: 0

    Information on RNA localisation is essential for understanding physiological and pathological processes, such as gene expression, cell reprogramming, host–pathogen interactions, and signalling pathways involving RNA transactions at the level

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    T Cell Clonal Analysis Using Single-cell RNA Sequencing and Reference Maps
    利用单细胞RNA测序和参考图谱进行T细胞克隆分析
    作者:  Massimo Andreatta, Paul Gueguen, Nicholas BorcherdingSantiago J. Carmona, 日期: 08/20/2023, 浏览量: 1105, Q&A: 0

    T cells are endowed with T-cell antigen receptors (TCR) that give them the capacity to recognize specific antigens and mount antigen-specific adaptive immune responses. Because TCR sequences are distinct in each naïve T cell, they serve as

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    Chromatin-RNA in situ Reverse Transcription Sequencing (CRIST-seq) Approach to Profile the Non-coding RNA Interaction Network
    染色质-RNA原位逆转录测序(CRIST-seq)方法分析非编码RNA相互作用网络
    作者:  Shilin Zhang, Xue Wen, Lei Zhou, Hui Li, Wei Li, Andrew R. Hoffman, Ji-Fan HuJiuwei Cui, 日期: 07/20/2023, 浏览量: 416, Q&A: 0

    Non-coding RNAs (ncRNAs) are defined as RNAs that do not encode proteins, but many ncRNAs do have the ability to regulate gene expression. These ncRNAs play a critical role in the epigenetic regulation of various physiological and pathological

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    Lipidomics Workflow for Analyzing Lipid Profiles Using Multiple Reaction Monitoring (MRM) in Liver Homogenate of Mice with Non-alcoholic Steatohepatitis (NASH)
    使用多重反应监测(MRM)分析非酒精性脂肪性肝炎(NASH)小鼠肝匀浆中脂质分布的脂质组学工作流程
    作者:  Hai Ning Wee, Lye Siang Lee, Sharon Hong Yu Han, Jin Zhou, Paul Michael YenJianhong Ching, 日期: 07/05/2023, 浏览量: 360, Q&A: 0

    Non-alcoholic steatohepatitis (NASH) is a condition characterized by inflammation and hepatic injury/fibrosis caused by the accumulation of ectopic fats in the liver. Recent advances in lipidomics have allowed the identification and characterization

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    Revised iCLIP-seq Protocol for Profiling RNA–protein Interaction Sites at Individual Nucleotide Resolution in Living Cells
    用于在活细胞中以单个核苷酸分辨率分析RNA-蛋白质相互作用位点的修订iCLIP-seq方案
    作者:  Syed Nabeel-ShahJack F. Greenblatt, 日期: 06/05/2023, 浏览量: 1067, Q&A: 0

    Individual nucleotide resolution UV cross-linking and immunoprecipitation followed by high-throughput sequencing (iCLIP-seq) is a powerful technique that is used to identify RNA-binding proteins’ (RBP) binding sites on target RNAs and to

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    Metagenomic Protocol (From Quality Control to Mapping) for Metagenome-assembled Genomes Using Anvi’o
    基于Anvi'o宏基因组组装的宏基因组方案(从质量控制到绘图)
    作者:  Soumyadev Sarkar, Tanner RichieSonny T. M. Lee, 日期: 05/20/2023, 浏览量: 71, Q&A: 0

    Synapses provide the main route of signal transduction within neuronal networks. Many factors regulate critical synaptic functions. These include presynaptic calcium channels, triggering neurotransmitter release, and postsynaptic ionotropic

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    Visualizing, Binning, and Refining of Metagenome-assembled Genomes (MAGs) with Anvi’o
    利用Anvi'o对元基因组组装的基因组(MAG)进行可视化、分档和提炼
    作者:  Soumyadev Sarkar, Tanner RichieSonny T. M. Lee, 日期: 05/20/2023, 浏览量: 161, Q&A: 0

    High throughput ‘omics technologies generate huge datasets that need to be properly analyzed in order to decipher the biological implications. The workflow of handling such datasets must be user friendly to facilitate rapid analysis. Here, we

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    Rapid Multiplexed Flow Cytometric Validation of CRISPRi sgRNAs in Tissue Culture
    组织培养中CRISPRi sgRNAs的快速多通道流式细胞术验证
    作者:  John S. Chorba, Vivian Q. Xia, Geoffrey A. SmithArun Padmanabhan, 日期: 01/20/2023, 浏览量: 728, Q&A: 0

    Genome-wide CRISPR-based screening is a powerful tool in forward genetics, enabling biologic discovery by linking a desired phenotype to a specific genetic perturbation. However, hits from a genome-wide screen require individual validation to

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