植物科学

分类

    Fractionation and Extraction of Crude Nuclear Proteins From Arabidopsis Seedlings
    拟南芥幼苗核蛋白的分离与提取
    作者:  Jiajia Zhao, Jinsong BaoFeifei Xu, 日期: 01/20/2022, 浏览量: 123, Q&A: 0

    The plant nucleus is an important subcellular organelle that contains the genome, ribosomal RNA, and regulatory proteins, and performs a central role in the functioning and metabolism of the cell. Fractionation of intact nuclei is a crucial process

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    Rhizoctonia solani Infection Assay of Young Sugar Beet and Arabidopsis plantlets
    甜菜和拟南芥幼苗丝核枯菌侵染试验
    作者:  Fredrik Dölfors, Louise Holmquist, Georgios TzelepisChristina Dixelius, 日期: 01/20/2022, 浏览量: 304, Q&A: 0

    Rhizoctonia solani is a soil-borne fungus, which rarely produces any spores in culture. Hence, all inoculation procedures are based on mycelia, often as a coat on cereal kernels, placed in close vicinity to the plant to be infected. In this

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    Bacterial Infection and Hypersensitive Response Assays in Arabidopsis-Pseudomonas syringae Pathosystem
    拟南芥-丁香假单胞菌病理系统中的细菌感染和超敏反应测定
    作者:  Minhang YuanXiu-Fang Xin, 日期: 12/20/2021, 浏览量: 603, Q&A: 0

    Arabidopsis thaliana-Pseudomonas syringae pathosystem has been used as an important model system for studying plant-microbe interactions, leading to many milestones and breakthroughs in the understanding of plant immune system and pathogenesis

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    Non-invasive Imaging of Rice Roots in Non-compacted and Compacted Soil
    非压实和压实土壤中水稻根系的无创成像
    作者:  Bipin K. Pandey, Jonathan A. AtkinsonCraig J. Sturrock, 日期: 12/20/2021, 浏览量: 1234, Q&A: 0

    Roots are the prime organ for nutrient and water uptake and are therefore fundamental to the growth and development of plants. However, physical challenges of a heterogeneous environment and diverse edaphic stresses affect root growth in soil.

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    An in vitro Coupled Assay for PEPC with Control of Bicarbonate Concentration
    一种控制碳酸氢盐浓度的磷酸烯醇式丙酮酸羧化酶的体外偶联分析
    作者:  Nicholas R. Moody, Chatawal PhansopalJames D. Reid, 日期: 12/20/2021, 浏览量: 366, Q&A: 0

    Phosphoenolpyruvate carboxylase (PEPC) catalyzes a critical step in carbon metabolism in plants and bacteria, the irreversible reaction between bicarbonate and phosphoenolpyruvate to produce the C4 compound oxaloacetate. This enzyme is particularly

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    Isolation of Plant Nuclei Compatible with Microfluidic Single-nucleus ATAC-sequencing
    与微流体单核 ATAC 测序兼容的植物核的分离
    作者:  Sandra B. Thibivilliers, Dirk K. AndersonMarc Y. Libault, 日期: 12/05/2021, 浏览量: 685, Q&A: 0

    Gene expression depends on the binding of transcription factors with DNA regulatory sequences. The level of accessibility for these sequences varies between cells and cell types. Until recently, using the Tn5 assay for transposase-accessible

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    Split-luciferase Complementation Imaging Assay to Study Protein-protein Interactions in Nicotiana benthamiana
    分裂荧光素酶互补成像分析研究本氏烟草中的蛋白质之间相互作用
    作者:  Liping Wang, Gang Yu, Alberto P. MachoRosa Lozano-Durán, 日期: 12/05/2021, 浏览量: 889, Q&A: 0

    The experimental identification of protein-protein interactions (PPIs) is critical to understand protein function. Thus, a plethora of sensitive and versatile approaches have been developed to detect PPIs in vitro or in vivo, such as protein

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    Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy
    利用荧光脂质和共聚焦显微镜对拟南芥幼苗的脂质摄取进行成像
    作者:  Rosa L. López-MarquésThomas G. Pomorski, 日期: 11/20/2021, 浏览量: 1245, Q&A: 0

    Eukaryotic cells use a diverse set of transporters to control the movement of lipids across their plasma membrane, which drastically affects membrane properties. Various tools and techniques to analyze the activity of these transporters have been

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    Preparation and Transfection of Populus tomentosa Mesophyll Protoplasts
    杨树中间叶原生质体的制备和转染
    作者:  Hou-Ling Wang, Ting Wang, Qi Yang, Weilun Yin, Xinli Xia, Hongwei GuoZhonghai Li, 日期: 11/20/2021, 浏览量: 1091, Q&A: 0

    Mesophyll protoplasts freshly isolated from leaves are a useful research system in plants. However, cell walls in woody plants contain more pectin, making mesophyll protoplasts isolation difficult in Populus. This has limited their application in

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    Enrichment of Cytoplasmic RNA Granules from Arabidopsis Seedlings
    从拟南芥幼苗中富集细胞质 RNA 颗粒
    作者:  Zhen Lei, Eun Yu KimJungnam Cho, 日期: 11/05/2021, 浏览量: 802, Q&A: 0

    RNA granules (RGs) are membraneless intracellular compartments that play important roles in the post-transcriptional control of gene expression. Stress granules (SGs) are a type of RGs that form under environmental challenges and/or internal

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