细胞生物学

分类

    Analysis of Leukemia Cell Metabolism through Stable Isotope Tracing in Mice
    小鼠白血病细胞代谢的稳定同位素示踪分析
    作者:  Nick van Gastel, Jessica B. Spinelli, Marcia C. HaigisDavid T. Scadden, 日期: 10/05/2021, 浏览量: 753, Q&A: 0

    Once thought to be a mere consequence of the state of a cell, intermediary metabolism is now recognized as a key regulator of mammalian cell fate and function. In addition, cell metabolism is often disturbed in malignancies such as cancer, and

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    Structural and Functional Mapping of Mesenchymal Bodies
    间充质体的结构和功能图谱

    The formation of spheroids with mesenchymal stem/stromal cells (MSCs), mesenchymal bodies (MBs), is usually performed using bioreactors or conventional well plates. While these methods promote the formation of a large number of spheroids, they

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    Phalloidin Staining of Actin Filaments for Visualization of Muscle Fibers in Caenorhabditis elegans
    秀丽隐杆线虫肌动蛋白丝的Phalloidin染色用于肌纤维可视化
    作者:  Mario RomaniJohan Auwerx, 日期: 10/05/2021, 浏览量: 667, Q&A: 0

    Advances in C. elegans research have allowed scientists to recapitulate different human disorders, from neurodegenerative diseases to muscle dysfunction, in these nematodes. Concomitantly, the interest in visualizing organs affected by these

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    Isolation, Culture, and Identification of Primary Müller Cells from Human Retina
    人视网膜原代 Müller 细胞的分离、培养和鉴定
    作者:  Yingying Chen, Ting Zhang, Shaoxue Zeng, Michelle Yam, Mark C. GilliesLing Zhu, 日期: 10/05/2021, 浏览量: 356, Q&A: 0

    Müller cells, the major glial cells of the retina, play vital roles in maintaining redox homeostasis and retinal metabolism. An immortalized human Müller cell line (MIO-M1) is widely used as an in vitro model to study Müller cells’

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    Calcium Imaging in T Lymphocytes: a Protocol for Use with Genetically Encoded or Chemical Ca2+ Indicators
    T 淋巴细胞中的钙成像:与遗传编码或化学 Ca2+ 指示一起使用的实验方案
    作者:  Amit JairamanMichael D. Cahalan, 日期: 10/05/2021, 浏览量: 459, Q&A: 0

    Elevations in cytosolic calcium (Ca2+) drive a wide array of immune cell functions, including cytokine production, gene expression, and cell motility. Live-cell imaging of cells loaded with ratiometric chemical Ca2+ indicators remains the gold

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    Isolation of Microvesicles from Human Circulating Neutrophils
    人循环中性粒细胞微泡的分离

    Neutrophil-derived microvesicles (NDMVs) are liberated by neutrophils upon cell activation by molecules. Once activated, neutrophils are primarily involved in acute inflammation; however, the microvesicles they produce are largely anti-inflammatory.

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    Fixation and Immunostaining of Endogenous Proteins or Post-translational Modifications in Caenorhabditis elegans
    秀丽隐杆线虫内源性蛋白质的固定和免疫染色或翻译后修饰
    作者:  Robert O'HaganIrini Topalidou, 日期: 10/05/2021, 浏览量: 687, Q&A: 0

    Although the advent of genetically-encoded fluorescent markers, such as the green fluorescent protein (GFP; Chalfie et al., 1994), has enabled convenient visualization of gene expression in vivo, this method is generally not effective for detecting

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    Isolation of Primary Cytotrophoblasts From Human Placenta at Term
    足月人胎盘原代细胞滋养层细胞的分离
    作者:  Arthur Colson, Christophe Louis Depoix, Corinne HubinontFrédéric Debiève, 日期: 10/05/2021, 浏览量: 314, Q&A: 0

    The placenta is a multifaceted organ, fulfilling critical functions for the fetus and the mother. Therefore, it is a critical regulator of the pregnancy, and its dysfunction leads to diseases, including fetal growth restriction and preeclampsia.

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    Rapid Isolation and Flow Cytometry Analysis of Murine Intestinal Immune Cells after Chemically Induced Colitis
    化学诱导结肠炎后鼠肠道免疫细胞的快速分离和流式细胞术分析
    作者:  Ashish K. Singh, Alfonso Blanco, Ray SinnottUlla G. Knaus, 日期: 10/05/2021, 浏览量: 86, Q&A: 0

    Chemically induced murine colitis models are widely used to understand intestinal homeostasis and inflammatory responses during acute and chronic gut inflammation such as Inflammatory Bowel Disease (IBD). Resident populations of immune cells

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    Identification and Quantitation of Neutrophil Extracellular Traps in Human Tissue Sections
    人体组织切片中中性粒细胞胞外陷阱的鉴定与定量
    作者:  Coraline Radermecker, Alexandre Hego, Philippe DelvenneThomas Marichal, 日期: 09/20/2021, 浏览量: 767, Q&A: 0

    Neutrophils are one of the first innate immune cells recruited to tissues during inflammation. An important function of neutrophils relies on their ability to release extracellular structures, known as Neutrophil Extracellular Traps or NETs, into

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