分子生物学

分类

    Gene Expression Analysis in Stem Cell-derived Cortical Neuronal Cultures Using Multi-well SYBR Green Quantitative PCR Arrays
    使用多孔 SYBR Green 定量 PCR 阵列在干细胞衍生的皮质神经元培养物中进行基因表达分析
    作者:  Vasavi Nallur Srinivasaraghavan, Faria ZafarBirgitt Schüle, 日期: 01/05/2022, 浏览量: 183, Q&A: 0

    To optimize differentiation protocols for stem cell-based in vitro modeling applications, it is essential to assess the change in gene expression during the differentiation process. This allows controlling its differentiation efficiency into the

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    Selection of Vaccinia Virus Recombinants Using CRISPR/Cas9
    使用 CRISPR/Cas9 筛选牛痘病毒重组体
    作者:  Anjali Gowripalan, Stewart A. SmithDavid C. Tscharke, 日期: 12/20/2021, 浏览量: 471, Q&A: 0

    The engineering of poxvirus genomes is fundamental to primary and applied virology research. Indeed, recombinant poxviruses form the basis for many novel vaccines and virotherapies but producing and purifying these viruses can be arduous. In recent

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    Isolation of Plant Nuclei Compatible with Microfluidic Single-nucleus ATAC-sequencing
    与微流体单核 ATAC 测序兼容的植物核的分离
    作者:  Sandra B. Thibivilliers, Dirk K. AndersonMarc Y. Libault, 日期: 12/05/2021, 浏览量: 608, Q&A: 0

    Gene expression depends on the binding of transcription factors with DNA regulatory sequences. The level of accessibility for these sequences varies between cells and cell types. Until recently, using the Tn5 assay for transposase-accessible

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    Measurement of DNA Damage Using the Neutral Comet Assay in Cultured Cells
    在培养细胞中使用中性彗星试验测量 DNA 损伤
    作者:  Elena Clementi, Zuzana GarajovaEnni Markkanen, 日期: 11/20/2021, 浏览量: 1267, Q&A: 0

    Maintenance of DNA integrity is of pivotal importance for cells to circumvent detrimental processes that can ultimately lead to the development of various diseases. In the face of a plethora of endogenous and exogenous DNA damaging agents, cells

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    Lentivirus-mediated Conditional Gene Expression
    慢病毒介导的条件基因表达
    作者:  Leah Rommereim, Ajay Suresh Akhade, Ronald N. Germain, Iain D. C. FraserNaeha Subramanian, 日期: 11/05/2021, 浏览量: 1222, Q&A: 0

    The ability to identify the role of a particular gene within a system is dependent on control of the expression of that gene. In this protocol, we describe a method for stable, conditional expression of Nod-Like receptors (NLRs) in THP-1 cells using

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    Histone modification ChIP-seq on Arabidopsis thaliana Plantlets
    拟南芥植株上的组蛋白修饰ChIP-seq
    作者:  Juan S. Ramirez-Prado, David LatrasseMoussa Benhamed, 日期: 11/05/2021, 浏览量: 880, Q&A: 1

    Characterizing the molecular mechanisms regulating gene expression is crucial for understanding the regulatory processes underlying physiological responses to environmental and developmental signals in eukaryotes. The covalent modification of

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    A Nucleocapsid-based Transcomplementation Cell Culture System of SARS-CoV-2 to Recapitulate the Complete Viral Life Cycle
    基于核衣壳的 SARS-CoV-2 转互补细胞培养系统,重现完整的病毒生命周期
    作者:  Yanying Yu, Xiaohui JuQiang Ding, 日期: 11/05/2021, 浏览量: 736, Q&A: 0

    The ongoing COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). As this virus is classified as a biosafety level-3 (BSL-3) agent, the development of countermeasures and basic research methods is logistically

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    A Rapid Induction Overexpression System for the Fission Yeast Schizosaccharomyces pombe
    裂解酵母裂殖酵母的快速诱导过表达系统
    作者:  Angad Garg, 日期: 10/20/2021, 浏览量: 431, Q&A: 0

    The fission yeast Schizosaccharomyces pombe is an excellent genetically tractable model organism used in the study of conserved eukaryotic cellular biology. One genetic tool in the assessment of gene function is the in vivo overexpression of

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    A High-throughput Pipeline to Determine DNA and Nucleosome Conformations by AFM Imaging
    通过 AFM 成像确定 DNA 和核小体构象的高通量管道
    作者:  Sebastian F. Konrad, Willem VanderlindenJan Lipfert, 日期: 10/05/2021, 浏览量: 861, Q&A: 0

    Atomic force microscopy (AFM) is a powerful tool to image macromolecular complexes with nanometer resolution and exquisite single-molecule sensitivity. While AFM imaging is well-established to investigate DNA and nucleoprotein complexes, AFM studies

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