生物化学

分类

    Anaerobic Expression and Purification of Holo-CCIS, an Artificial Iron-sulfur Protein
    人工铁硫蛋白 Holo-CCIS的厌氧表达与纯化
    作者:  Bhanu P. Jagilinki, Irina Paluy, Vikas NandaDror Noy, 日期: 09/20/2021, 浏览量: 63, Q&A: 0

    Iron-sulfur proteins are ubiquitous among all living organisms and are indispensable for almost all metabolic pathways ranging from photosynthesis, respiration, nitrogen, and carbon dioxide cycles. The iron-sulfur clusters primarily serve as

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    Methods for the Extraction of Heme Prosthetic Groups from Hemoproteins
    从血红素中提取血红素辅基的方法
    作者:  Kat Ellis-Guardiola, Jess SouleRobert T. Clubb, 日期: 09/20/2021, 浏览量: 114, Q&A: 0

    Hemoproteins are widely researched because they contain redox-active heme prosthetic groups (iron + protoporphyrin IX) that enable them to perform a range of vital functions, acting as enzymes, participants in electron transfer reactions, or gas

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    Cell-free Translation: Preparation and Validation of Translation-competent Extracts from Saccharomyces cerevisiae
    无细胞翻译:酿酒酵母翻译活性提取物的制备和验证
    作者:  Brandon M. Trainor, Anton A. Komar, Dimitri G. PestovNatalia Shcherbik, 日期: 09/20/2021, 浏览量: 77, Q&A: 0

    Cell-free translation is a powerful technique for in vitro protein synthesis. While cell-free translation platforms prepared from bacterial, plant, and mammalian cells are commercially available, yeast-based translation systems remain proprietary

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    Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays
    利用组蛋白肽阵列评估组蛋白修饰阅读蛋白的体外结合特异性
    作者:  Mark W. SooArneet L. Saltzman, 日期: 09/20/2021, 浏览量: 53, Q&A: 0

    In the field of chromatin biology, a major goal of understanding the roles of histone post-translational modifications is to identify the proteins and domains that recognize these modifications. Synthetic histone peptides containing one or more

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    A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin
    一种高效生产和Src激酶介导的马海兔磷酸化的共纯化方法
    作者:  Sherlene L. Brown, Yuan Ren, Daniel M. SuterSeema Mattoo, 日期: 09/20/2021, 浏览量: 101, Q&A: 0

    Cortactin is an actin-binding protein that regulates processes like cell migration, endocytosis, and tumor cell metastasis. Although cortactin is associated with actin-cytoskeletal dynamics in non-neuronal cells and cell-free systems, the exact

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    Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids
    从针叶树类囊体中分离光合巨复合体和超复合体的增溶方法
    作者:  Pushan Bag, Wolfgang P. Schröder, Stefan JanssonDomenica Farci, 日期: 09/05/2021, 浏览量: 477, Q&A: 0

    Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades. In higher plants, the process takes place in the thylakoid

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    Monitoring Protein Splicing Using In-gel Fluorescence Immediately Following SDS-PAGE
    SDS-PAGE后立即使用凝胶内荧光监测蛋白质剪接
    作者:  Joel Weinberger IIChristopher W. Lennon, 日期: 08/20/2021, 浏览量: 1149, Q&A: 0

    Inteins garner significant interest from both basic and applied researchers due to their unique catalytic abilities. Herein, we describe a protocol for accurately monitoring protein splicing without purification using in-gel fluorescence immediately

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    Construction of a Highly Diverse mRNA Library for in vitro Selection of Monobodies
    体外选择单体的高度多样性mRNA文库的构建

    Recently, we developed transcription/translation coupled with the association of puromycin linker (TRAP) display as a quick in vitro selection method to obtain antibody-like proteins. For the in vitro selection, it is important to prepare mRNA

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    Synchronized Real-time Measurement of Sec-mediated Protein Translocation
    Sec介导的蛋白质易位的同步实时测量
    作者:  Riti Gupta, Dmitri ToptyginChristian M. Kaiser, 日期: 08/20/2021, 浏览量: 656, Q&A: 0

    The Sec translocon, consisting of a heterotrimeric transmembrane channel (SecYEG) and an associated ATPase (SecA), catalyzes the export of unfolded proteins from the cytosol in bacteria. Kinetically resolving protein translocation at high resolution

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    A Novel Method to Make Polyacrylamide Gels with Mechanical Properties Resembling those of Biological Tissues
    一种制备具有类似生物组织力学性能的聚丙烯酰胺凝胶的新方法  
    作者:  Katarzyna Pogoda, Elisabeth E. CharrierPaul A. Janmey, 日期: 08/20/2021, 浏览量: 935, Q&A: 0

    Studies characterizing how cells respond to the mechanical properties of their environment have been enabled by the use of soft elastomers and hydrogels as substrates for cell culture. A limitation of most such substrates is that, although

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