微生物学

分类

    PCR-mediated One-day Synthesis of Guide RNA for the CRISPR/Cas9 System
    CRISPR/Cas9系统的PCR介导的一日合成向导RNA
    作者:  Naim Hassan, Farhana Easmin, Keisuke EkinoSatoshi Harashima, 日期: 07/05/2021, 浏览量: 1159, Q&A: 0

    Nowadays, CRISPR (clustered regularly interspaced short palindromic repeats) and the CRISPR-associated protein (Cas9) system play a major role in genome editing. To target the desired sequence of the genome successfully, guide RNA (gRNA) is

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    Transcriptional Run-on: Measuring Nascent Transcription at Specific Genomic Sites in Yeast
    连缀转录:测定酵母特定基因组位点的新生转录
    作者:  Victoria Begley, Lola de Miguel-JiménezSebastián Chávez, 日期: 06/20/2021, 浏览量: 761, Q&A: 0

    DNA transcription by RNA polymerases has always interested the scientific community as it is one of the most important processes involved in genome expression. This has led scientists to come up with different protocols allowing analysis of this

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    Defined Mutant Library Sequencing (DML-Seq) for Identification of Conditional Essential Genes
    确定突变体文库测序(DML-Seq)用于鉴定的条件必需基因
    作者:  Shuai Shao, Lifan Wei, Feng Xia, Yuanxing ZhangQiyao Wang, 日期: 03/05/2021, 浏览量: 1771, Q&A: 0

    Transposon insertion sequencing (TIS) is an emerging technique which utilizes a massive transposon mutant library to screen specific phenotype and determine the conditional essential genetic requirements for bacterial fitness under distinct

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    Bacterial Conjugation Protocol for Ruminant Mycoplasmas
    反刍动物支原体细菌接合的实验方法
    作者:  Eveline Sagné, Christine CittiEmilie Dordet-Frisoni, 日期: 01/20/2021, 浏览量: 857, Q&A: 0

    In Mycoplasma agalactiae, two simultaneous processes of DNA transfer have been described that require direct cell-to-cell contact and are similar to conjugation. One involves the self-transmission of an integrative conjugative element (ICE) while

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    土壤和水体环境T4型细菌病毒g23基因多样性研究
    Genetic Diversity of the Major Capsid Genes (g23) of T4-type Bacteriophages in Soil and Water Environments
    作者:  刘俊杰, 荆瑞勇王光华, 日期: 01/10/2021, 浏览量: 599, Q&A: 0
    摘要: 2005年Filée等人首次报道了编码主要壳蛋白的g23基因可用于解析海洋水体T4型细菌病毒基因多样性。随后众多研究表明,该基因还可以应用于其他环境中细菌病毒多样性和群落结构组成分析 (王光华,2020) ...
    微生物群落胞内/胞外吸附/胞外游离水环境DNA的分离提取
    Isolation and Extraction of Intracellular, Absorbed-extracellular and Free-extracellular Environmental DNA from Aquatic Microbial Community
    作者:  赵泽鞠峰, 日期: 12/25/2020, 浏览量: 1115, Q&A: 0
    摘要:胞外DNA是位于微生物细胞膜外的DNA片段,来自于微生物的主动/被动排放或细胞裂解,作为微菌落的结构成分发挥着重要的作用。本实验流程通过过滤、洗脱再过滤的二级处理将环境水体中微生物组的胞内/胞外吸附/胞外游离DNA分离,并且在十六烷基三甲基溴化铵 ...
    Candida albicans Culture, Cell Harvesting, and Total RNA Extraction
    白念珠菌培养、细胞收获和总RNA提取
    作者:  Max V. CravenerAaron P. Mitchell, 日期: 11/05/2020, 浏览量: 1351, Q&A: 0

    Transcriptional analysis has become a cornerstone of biological research, and with the advent of cheaper and more efficient sequencing technology over the last decade, there exists a need for high-yield and efficient RNA extraction techniques. Fungi

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    Estimation of the Minimum Number of Replication Origins Per Chromosome in any Organism
    任何生物体内每个染色体的最小复制起源点数目的估计
    作者:  Marcelo S. da Silva, 日期: 10/20/2020, 浏览量: 1467, Q&A: 0
    Eukaryote nuclear genomes predominantly replicate through multiple replication origins. The number of replication origins activated per chromosome during the S-phase duration may vary according to many factors, but the predominant one is replication ...
    TetR Regulated in vivo Repression Technology to Identify Conditional Gene Silencing in Genetically Engineerable Bacteria Using Vibrio cholerae Murine Infections as Model System
    以小鼠霍乱弧菌感染为模型系统的TetR调控体内抑制技术鉴定基因工程菌的条件基因沉默
    作者:  Franz G. Zingl, Fabian Mitterer, Himadri B. ThapaStefan Schild, 日期: 10/05/2020, 浏览量: 1008, Q&A: 0
    Investigation of bacterial gene regulation upon environmental changes is still a challenging task. For example, Vibrio cholerae, a pathogen of the human gastrointestinal tract, faces diverse transient conditions in different compartments ...
    Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria
    细菌转录起始位点的差异RNA序列全基因组鉴定
    作者:  Ramón Cervantes-RiveraAndrea Puhar, 日期: 09/20/2020, 浏览量: 2713, Q&A: 0
    Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are ...