神经科学

分类

    A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin
    一种高效生产和Src激酶介导的马海兔磷酸化的共纯化方法
    作者:  Sherlene L. Brown, Yuan Ren, Daniel M. SuterSeema Mattoo, 日期: 09/20/2021, 浏览量: 66, Q&A: 0

    Cortactin is an actin-binding protein that regulates processes like cell migration, endocytosis, and tumor cell metastasis. Although cortactin is associated with actin-cytoskeletal dynamics in non-neuronal cells and cell-free systems, the exact

    ...
    Measurement of LRRK2 Kinase Activity by Proximity Ligation Assay
    通过邻近连接测定法测量 LRRK2 激酶活性
    作者:  Matthew T. Keeney, Eric K. Hoffman, J. Timothy GreenamyreRoberto Di Maio, 日期: 09/05/2021, 浏览量: 438, Q&A: 0

    Missense mutations in leucine rich-repeat kinase 2 (LRRK2) cause forms of familial Parkinson’s disease and have been linked to ‘idiopathic’ Parkinson’s disease. Assessment of LRRK2 kinase activity has been very challenging

    ...
    Isolation of Microglia and Analysis of Protein Expression by Flow Cytometry: Avoiding the Pitfall of Microglia Background Autofluorescence
    小胶质细胞的分离及蛋白表达的流式细胞仪分析:避免小胶质细胞背景荧光的陷阱
    作者:  Jeremy C. Burns, Richard M. RansohoffMichaël Mingueneau, 日期: 07/20/2021, 浏览量: 1470, Q&A: 0

    Microglia are a unique type of tissue-resident innate immune cell found within the brain, spinal cord, and retina. In the healthy nervous system, their main functions are to defend the tissue against infectious microbes, support neuronal networks

    ...
    A Method for Estimating the Potential Synaptic Connections Between Axons and Dendrites From 2D Neuronal Images
    一种从二维神经元图像中估计轴突和树突之间潜在突触连接的方法
    作者:  Carolina Tecuatl, Diek W. WheelerGiorgio A. Ascoli, 日期: 07/05/2021, 浏览量: 1577, Q&A: 0

    Computational neuroscience aims to model, reproduce, and predict network dynamics for different neuronal ensembles by distilling knowledge derived from electrophysiological and morphological evidence. However, analyses and simulations often remain

    ...
    Immunofluorescence of GFAP and TNF-α in the Mouse Hypothalamus
    GFAP和TNF-α在小鼠下丘脑的免疫荧光
    作者:  Prasad S. DalviDenise D. Belsham, 日期: 07/05/2021, 浏览量: 950, Q&A: 0

    Immunofluorescence is a reliable method for identifying specific proteins in neuronal and glial cell populations of the hypothalamus. Several immunofluorescence protocols are available to detect protein markers and neuropeptides in the hypothalamus;

    ...
    Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease
    用于研究人类疾病中蛋白质N-糖基化失调的N-糖肽丰度和N-糖基化位点的差异分析
    作者:  Qi Zhang, Cheng Ma, Lian LiLih-Shen Chin, 日期: 06/20/2021, 浏览量: 1069, Q&A: 0

    Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass

    ...
    A Lipidomics Approach to Measure Phosphatidic Acid Species in Subcellular Membrane Fractions Obtained from Cultured Cells
    用脂质组学方法测定培养细胞亚细胞膜组分中磷脂酸的种类
    作者:  Nawal Kassas, Laetitia Fouillen, Stéphane GasmanNicolas Vitale, 日期: 06/20/2021, 浏览量: 1145, Q&A: 0

    Over the last decade, lipids have emerged as possessing an ever-increasing number of key functions, especially in membrane trafficking. For instance, phosphatidic acid (PA) has been proposed to play a critical role in different steps along the

    ...
    Dual Color, Live Imaging of Vesicular Transport in Axons of Cultured Sensory Neurons
    培养感觉神经元轴突囊泡运输的双色实时成像
    作者:  Yoko BekkuJames L. Salzer, 日期: 06/20/2021, 浏览量: 1086, Q&A: 0

    The function of neurons in afferent reception, integration, and generation of electrical activity relies on their strikingly polarized organization, characterized by distinct membrane domains. These domains have different compositions resulting from

    ...
    In vivo Fluorescence Imaging of Extracellular ATP in the Mouse Cerebral Cortex with a Hybrid-type Optical Sensor
    混合型光传感器对小鼠大脑皮层细胞外ATP的体内荧光成像

    Adenosine 5’-triphosphate (ATP) works as an extracellular signaling molecule for cells in the brain, such as neurons and glia. Cellular communication via release of ATP is involved in a range of processes required for normal brain functions,

    ...
    A New Method for Studying RNA-binding Proteins on Specific RNAs
    一种研究特异性RNA结合蛋白的新方法
    作者:  Weiping Sun, Ziheng Zhang, Ji-Long LiuMin Zhuang, 日期: 05/20/2021, 浏览量: 6343, Q&A: 0

    Proximity-based protein labeling has been developed to identify protein-nucleic acid interactions. We have reported a novel method termed CRUIS (CRISPR-based RNA-United Interacting System), which captures RNA-protein interactions in living cells by

    ...