分子生物学

分类

    Advances in Proximity Ligation in situ Hybridization (PLISH)
    PLISH技术的研究进展
    作者:  Monica Nagendran, Adam M Andruska, Pehr B HarburyTushar J Desai, 日期: 11/05/2020, 浏览量: 2144, Q&A: 0
    Understanding tissues in the context of development, maintenance and disease requires determining the molecular profiles of individual cells within their native in vivo spatial context. We developed a Proximity Ligation in situ ...
    Mapping mRNA-18S rRNA Contacts Within Translation Initation Complex by Means of Reverse Transcriptase Termination Sites and RNAseq
    用逆转录酶终止位点和RNAseq定位翻译起始复合体内的mRNA-18S rRNA接触点
    作者:  Irene Díaz-López, René ToribioIván Ventoso, 日期: 08/20/2020, 浏览量: 1705, Q&A: 0
    The nucleotides involved in RNA-RNA interaction can be tagged by chemical- or UV-induced crosslinking, and further identified by classical or modern high throughput techniques. The contacts of mRNA with 18S rRNA that occur along the mRNA channel of ...
    Confocal and Super-resolution Imaging of RNA in Live Bacteria Using a Fluorogenic Silicon Rhodamine-binding Aptamer
    含氟硅-罗丹明适配体用于活性菌中RNA的激光共聚焦超分辨率成像
    作者:  Regina Wirth, Peng Gao, G. Ulrich Nienhaus, Murat SunbulAndres Jäschke, 日期: 05/05/2020, 浏览量: 4247, Q&A: 0
    Genetically encoded light-up RNA aptamers have been shown to be promising tools for the visualization of RNAs in living cells, helping us to advance our understanding of the broad and complex life of RNA. Although a handful of light-up aptamers ...
    Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH
    利用显色原位杂交进行固定细胞和组织中单个RNA的检测
    作者:  Meng Jiang, Chen LinRongqin Ke, 日期: 02/05/2020, 浏览量: 2226, Q&A: 0
    Visualization of RNA molecules in situ helps to better understand the functions of expressed genes. Currently, most conventional in situ hybridization methods for visualization of individual RNAs are based on fluorescence ...
    A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol
    一种广泛应用的尿素荧光/比色mrna原位杂交技术
    作者:  Chiara Sinigaglia, 日期: 09/05/2019, 浏览量: 3465, Q&A: 0
    In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a ...
    Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs
    荧光polyU RNAs的酶促合成及分离
    作者:  Christian Beren, Katherine N. Liu, Lisa L. Dreesens, Charles M. KnoblerWilliam M. Gelbart, 日期: 09/05/2018, 浏览量: 2940, Q&A: 0
    The physical properties of viral-length polyuridine (PolyU) RNAs, which cannot base-pair and form secondary structures, are compared with those of normal-composition RNAs, composed of comparable numbers of each of A, U, G and C nucleobases. In this ...
    Modification of 3’ Terminal Ends of DNA and RNA Using DNA Polymerase θ Terminal Transferase Activity
    利用DNA聚合酶θ末端转移酶活性修饰DNA和RNA的3'末端
    作者:  Trung M. Hoang, Tatiana KentRichard T. Pomerantz, 日期: 06/20/2017, 浏览量: 7063, Q&A: 0
    DNA polymerase θ (Polθ) is a promiscuous enzyme that is essential for the error-prone DNA double-strand break (DSB) repair pathway called alternative end-joining (alt-EJ). During this form of DSB repair, Polθ performs terminal transferase activity ...
    In vitro Transcription (IVT) and tRNA Binding Assay
    体外转录(IVT)和tRNA结合试验
    作者:  Sonja MK Schoenfelder, 日期: 09/20/2014, 浏览量: 12205, Q&A: 0
    This protocol describes the coupling of (i) “live” in vitro RNA transcription with (ii) binding by a radiolabeled, pre-formed tRNA followed by native gel electrophoresis and phosphorimager scan to visualize the complex. The necessity arose ...
    Metabolic Labeling of Yeast RNA with Radioactive Uracil
    同位素示踪法进行酵母RNA的代谢标记
    作者:  Yuehua Wei, 日期: 04/05/2013, 浏览量: 8935, Q&A: 0
    To examine gene expression, Northern blot or Real-Time PCR can be used to detect low abundant RNA such as mRNA. However, for high abundant RNAs such as rRNA and tRNA, Northern blot will not be able to discriminate the newly synthesized RNA from ...
    Synthesis of 5’ end-labeled RNA
    5'末端标记RNA的合成
    作者:  Harald Putzer, 日期: 07/20/2012, 浏览量: 11865, Q&A: 2
    5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases ...