生物化学

分类

    Oil Red O Staining for Lipid Content in Caenorhabditis elegans
    油红O染色测定秀丽隐杆线虫脂质含量  
    作者:  Feng-Yung WangTsui-Ting Ching, 日期: 08/20/2021, 浏览量: 1029, Q&A: 0

    The nematode Caenorhabditis elegans has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has

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    Oral Fat Tolerance Test in Male and Female C57BL/6 Mice
    雄性和雌性C57Bl/6小鼠口服脂肪耐量试验

    Postprandial hyperlipidemia is an important risk factor for cardiovascular diseases, and it can be evaluated by an oral fat tolerance test (OFTT). There is no consensus on how to perform the OFTT in rodents, especially in the C57BL/6 mice strain.

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    小鼠粪便样本中短链脂肪酸的定量检测
    Quantitative Analysis of Short-chain Fatty Acids (SCFAs) in Mouse Fecal Sample
    作者:  刘红宾, 汤锦辉戴磊, 日期: 12/25/2020, 浏览量: 1647, Q&A: 1
    摘要:短链脂肪酸 (Short-chain fatty acids, SCFAs) ...
    Triacylglycerol Measurement in HeLa Cells
    HeLa细胞中三酰甘油的测定
    作者:  Ximing DuHongyuan Yang, 日期: 12/20/2020, 浏览量: 1575, Q&A: 0

    Lipid droplets store triacylglycerols (triglycerides) and sterol esters to regulate lipid and energy homeostasis. Triacylglycerol measurement is often performed during the investigation of lipid droplet formation and growth. This protocol describes

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    Total Triglyceride Quantification in Caenorhabditis elegans
    秀丽隐杆线虫总甘油三酯的定量研究
    作者:  Anjali SandhuVarsha Singh, 日期: 11/20/2020, 浏览量: 1985, Q&A: 0
    Several studies suggest an important role of lipid metabolism in regulating longevity of Caenorhabditis elegans. Therefore, assays to quantify lipids have enormous value in understanding aging and pathologies associated with it. ...
    GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues
    基于GC-MS法分析使用甲醇:氯仿从植物组织中提取的脂肪酸
    作者:  Manish Kumar Patel, Shubhashis DasJitendra Kumar Thakur, 日期: 09/20/2018, 浏览量: 10413, Q&A: 0
    Fatty acids (FAs) are carboxylic acids with long aliphatic chains that may be straight, branched and saturated or unsaturated. Most of the naturally occurring plant FAs contains an even number of carbon (C4-C24). FAs are used in food and ...
    Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)
    使用管饲法重组富含甘油三酯的脂蛋白颗粒(rTRL)来评估小鼠中放射性标记的胆固醇的摄取和生物分布
    作者:  Anna Worthmann, Clara JohnJoerg Heeren, 日期: 07/05/2018, 浏览量: 4015, Q&A: 0
    The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation ...
    Tracking Lipid Transfer by Fatty Acid Isotopolog Profiling from Host Plants to Arbuscular Mycorrhiza Fungi
    脂肪酸同位素标记谱分析追踪脂质从寄主植物到丛枝菌根真菌的转运
    作者:  Andreas Keymer, Claudia Huber, Wolfgang EisenreichCaroline Gutjahr, 日期: 04/05/2018, 浏览量: 5749, Q&A: 0
    Lipid transfer from host plants to arbuscular mycorrhiza fungi was hypothesized for several years because sequenced arbuscular mycorrhiza fungal genomes lack genes encoding cytosolic fatty acid synthase (Wewer et al., 2014; Rich et al. ...
    稻米的粗脂肪含量测定
    Determination of Crude Fat Content in Rice Grains
    作者:  彭波李一博, 日期: 03/16/2018, 浏览量: 3247, Q&A: 0
    实验原理:利用溶剂的回流和虹吸原理,对稻米中的粗脂肪成分进行连续提取。脂肪能溶于乙醚等有机溶剂的特性,将稻米的米粉放在索氏抽提器中用乙醚等有机溶剂连续提取(反复经过抽提、蒸发、冷凝、回流、抽提……循环过程),稻米中的脂肪在下部的烧瓶中逐渐浓集,直至将试样中的脂肪全部收集到烧瓶中 ...
    Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri
    凯氏小球藻中脂肪酸含量及三酰甘油组成
    作者:  Motohide AokiNorihiro Sato, 日期: 01/05/2018, 浏览量: 6635, Q&A: 0
    Triacylglycerols (TAGs) are esters formed from one glycerol and three fatty acids. TAGs are induced to accumulate in algal cells under environmental stress conditions including nutrient-limitation, hyperosmosis, and low temperature, for the storage ...