生物化学

分类

    Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids
    从针叶树类囊体中分离光合巨复合体和超复合体的增溶方法
    作者:  Pushan Bag, Wolfgang P. Schröder, Stefan JanssonDomenica Farci, 日期: 09/05/2021, 浏览量: 477, Q&A: 0

    Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades. In higher plants, the process takes place in the thylakoid

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    A Novel Method to Make Polyacrylamide Gels with Mechanical Properties Resembling those of Biological Tissues
    一种制备具有类似生物组织力学性能的聚丙烯酰胺凝胶的新方法  
    作者:  Katarzyna Pogoda, Elisabeth E. CharrierPaul A. Janmey, 日期: 08/20/2021, 浏览量: 935, Q&A: 0

    Studies characterizing how cells respond to the mechanical properties of their environment have been enabled by the use of soft elastomers and hydrogels as substrates for cell culture. A limitation of most such substrates is that, although

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    CENP-C Phosphorylation by CDK1 in vitro
    CDK1体外磷酸化CENP-C的研究
    作者:  Reito Watanabe, Masatoshi Hara, Mariko AriyoshiTatsuo Fukagawa, 日期: 01/05/2021, 浏览量: 2133, Q&A: 0

    Accurate chromosome segregation during mitosis requires the kinetochore, a large protein complex, which makes a linkage between chromosomes and spindle microtubes. An essential kinetochore component, CENP-C, is phosphorylated by Cyclin-B-Cyclin

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    Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina
    等电聚焦用于小鼠视网膜中磷酸化视紫红质的定量分析
    作者:  Sowmya Bekshe Lokappa, M. Carter CornwallJeannie Chen, 日期: 07/20/2019, 浏览量: 4224, Q&A: 0
    Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, ...
    Isolation of Thylakoid Membranes from the Cyanobacterium Synechocystis sp. PCC 6803 and Analysis of Their Photosynthetic Pigment-protein Complexes by Clear Native-PAGE
    从蓝藻集胞藻sp. PCC 6803中分离类囊体膜并利用CN-PAGE非变性聚丙烯酰胺凝胶电泳分析光合色素蛋白复合物
    作者:  Josef Komenda, Vendula KrynickáTomas Zakar, 日期: 01/05/2019, 浏览量: 4824, Q&A: 0
    Cyanobacteria represent a frequently used model organism for the study of oxygenic photosynthesis. They belong to prokaryotic microorganisms but their photosynthetic apparatus is quite similar to that found in algal and plant chloroplasts. The key ...
    Very Low Molecular Weight Proteins Electrophoresis Protocol
    极低分子量蛋白电泳实验方法
    作者:  César E. Rivera, José D. Rosales, Juan C. Freites-PerezEliaira Rodriguez, 日期: 11/20/2018, 浏览量: 10517, Q&A: 5
    The electrophoresis is the most used technique to separate proteins and usually the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proposed by Laemmli is the prefered since it can diferentiate large size proteins, but for very ...
    SDS-PAGE for Silk Fibroin Protein
    丝素蛋白的SDS-聚丙烯酰胺凝胶电泳
    作者:  Yu-Qing Zhang, 日期: 10/20/2018, 浏览量: 7796, Q&A: 0
    The method and detailed procedure of SDS-PAGE for silk proteins are exactly the same as for other proteins, but the electrophoresis profile of silk protein is often unsatisfactory. The main reason is that their molecular masses are too large, and ...
    Preparation of Cerebellum Granule Neurons from Mouse or Rat Pups and Evaluation of Clostridial Neurotoxin Activity and Their Inhibitors by Western Blot and Immunohistochemistry
    用老鼠或鼠仔制备小脑颗粒神经元并通过免疫印迹和免疫组织化学法评估梭菌神经毒素活性及其抑制剂
    作者:  Domenico Azarnia TehranMarco Pirazzini, 日期: 07/05/2018, 浏览量: 7892, Q&A: 0
    Cerebellar Granule Neurons (CGN) from post-natal rodents have been widely used as a model to study neuronal development, physiology and pathology. CGN cultured in vitro maintain the same features displayed in vivo by mature ...
    Detection of Catalase Activity by Polyacrylamide Gel Electrophoresis (PAGE) in Cell Extracts from Pseudomonas aeruginosa
    聚丙烯酰胺凝胶电泳(PAGE)检测铜绿假单胞菌细胞提取物中的过氧化氢酶活性
    作者:  Magdalena Pezzoni, Ramón A. PizarroCristina S. Costa, 日期: 06/05/2018, 浏览量: 6410, Q&A: 0
    Bacteria in nature and as pathogens commonly face oxidative stress which causes damage to proteins, lipids and DNA. This damage is produced by the action of reactive oxygen species (ROS) such as hydrogen peroxide (H2O2), ...
    蛋白质双向电泳
    Two-dimensional Gel Electrophoresis of Proteins
    作者:  马海港, 彭波袁猛, 日期: 03/16/2018, 浏览量: 4825, Q&A: 0
    实验原理:双向电泳(two-dimensional electropHoresis)技术是等电聚焦和十二烷基硫酸钠-聚丙烯酰胺(SDS-PAGE)凝胶电泳技术的组合,即先进行等电聚焦电泳(按照蛋白质等电点对蛋白质进行分离),然后再进行SDS-PAGE凝胶电泳 ...