生物化学

分类

    Amplex Red Assay for Measuring Hydrogen Peroxide Production from Caenorhabditis elegans
    利用Amplex Red检测秀丽隐杆线虫中过氧化氢的生成
    作者:  Ozgur Karakuzu, Melissa R. Cruz, Yi LiuDanielle A. Garsin, 日期: 11/05/2019, 浏览量: 6554, Q&A: 0
    Reagents such as Amplex® Red have been developed for detecting hydrogen peroxide (H2O2) and are used to measure the release of H2O2 from biological samples such as mammalian leukocytes ...
    Quantifying MAMP-induced Production of Reactive Oxygen Species in Sorghum and Maize
    高粱和玉米中由MAMP诱导产生的活性氧定量分析
    作者:  Rozalynne Samira, Xinye Zhang, Jennifer Kimball, Yaya Cui, Gary StaceyPeter J. Balint-Kurti, 日期: 07/20/2019, 浏览量: 2758, Q&A: 0
    Plants have the ability to recognize microbe-associated molecular patterns (MAMPs) and mount a defense response. The level of the MAMP response can vary depending on genetic and environmental factors. The most commonly studied MAMPs are flg22, a ...
    Hypochlorous Acid Staining with R19-S in the Drosophila Intestine upon Ingestion of Opportunistic Bacteria
    果蝇肠道摄入外来细菌后次氯酸R19-S染色
    作者:  Salma Hachfi, Olivia BenguettatArmel Gallet, 日期: 05/20/2019, 浏览量: 3740, Q&A: 0
    The intestine is endowed with an innate immune system that is required to fight any exogenous bacteria that are swallowed along with the food. The first line of defense that is mounted by the gut epithelium is the release of immune Reactive Oxygen ...
    活性氧检测
    Reactive Oxygen Detection
    作者:  游均, 方玉洁熊立仲, 日期: 04/27/2018, 浏览量: 9534, Q&A: 0
    第一部分:NBT染色检测超氧阴离子(O2-)

    实验原理:氮蓝四唑 (NBT, nitroblue ...
    Detection of Reactive Oxygen Species (ROS) in Cyanobacteria Using the Oxidant-sensing Probe 2’,7’-Dichlorodihydrofluorescein Diacetate (DCFH-DA)
    使用氧化物探针2',7'-二氯二氟荧光素二乙酸酯(DCFH-DA)检测蓝细菌中活性氧物质(ROS)
    作者:  Rajneesh, Jainendra Pathak, Ananya Chatterjee, Shailendra P. SinghRajeshwar P. Sinha, 日期: 09/05/2017, 浏览量: 17375, Q&A: 0
    Reactive oxygen species (ROS) are cell signaling molecules synthesized inside the cells as a response to routine metabolic processes. In stress conditions such as ultraviolet radiation (UVR), ROS concentration increases several folds in the cells ...
    Measuring Oxidative Stress in Caenorhabditis elegans: Paraquat and Juglone Sensitivity Assays
    秀丽隐杆线虫中的氧化胁迫测定:百草枯和胡桃酮敏感性分析法
    作者:  Megan M. Senchuk, Dylan J. Dues Jeremy M. Van Raamsdonk, 日期: 01/05/2017, 浏览量: 10893, Q&A: 0
    Oxidative stress has been proposed to be one of the main causes of aging and has been implicated in the pathogenesis of many diseases. Sensitivity to oxidative stress can be measured by quantifying survival following exposure to a reactive oxygen ...
    Detection of Reactive Oxygen Species in Oryza sativa L. (Rice)
    水稻中活性氧的检测
    作者:  Navdeep Kaur, Isha Sharma, Kamal KiratPratap Kumar Pati, 日期: 12/20/2016, 浏览量: 16082, Q&A: 2
    Superoxide ions (O2-) and hydrogen peroxide (H2O2) are the reactive oxygen species (ROS) that play a significant role in regulation of many plant processes. The level of O2- ions is determined ...
    Hydrogen Peroxide Measurement in Arabidopsis Root Tissue Using Amplex Red
    Amplex Red荧光法测定拟南芥根组织中的过氧化氢酶
    作者:  Tzvetina Brumbarova, Cham Thi Tuyet LePetra Bauer, 日期: 11/05/2016, 浏览量: 12384, Q&A: 0
    This protocol describes the measurement of hydrogen peroxide (H2O2) content in Arabidopsis root tissue by using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. When root tissue is disrupted and ...
    In vitro Real-time Measurement of the Intra-bacterial Redox Potential
    内部细菌氧化还原电位的体外实时测量
    作者:  Joris van der HeijdenB. Brett Finlay, 日期: 09/05/2015, 浏览量: 7599, Q&A: 0
    All bacteria that live in oxygenated environments have to deal with oxidative stress caused by some form of exogenous or endogenous reactive oxygen species (ROS) (Imlay, 2013). Large quantities of ROS damage DNA, lipids and proteins which can ...
    Electron Paramagnetic Resonance (EPR) Spectroscopy to Detect Reactive Oxygen Species in Staphylococcus aureus
    电子顺磁共振(EPR)光谱法检测金黄色葡萄球菌中的活性氧类物质
    Under aerobic conditions, Staphylococcus aureus (S. aureus) primarily metabolizes glucose to acetic acid. Although normally S. aureus is able to re-utilize acetate as a carbon source following glucose exhaustion, ...