微生物学

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    Transcriptional Run-on: Measuring Nascent Transcription at Specific Genomic Sites in Yeast
    连缀转录:测定酵母特定基因组位点的新生转录
    作者:  Victoria Begley, Lola de Miguel-JiménezSebastián Chávez, 日期: 06/20/2021, 浏览量: 761, Q&A: 0

    DNA transcription by RNA polymerases has always interested the scientific community as it is one of the most important processes involved in genome expression. This has led scientists to come up with different protocols allowing analysis of this

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    Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria
    细菌转录起始位点的差异RNA序列全基因组鉴定
    作者:  Ramón Cervantes-RiveraAndrea Puhar, 日期: 09/20/2020, 浏览量: 2713, Q&A: 0
    Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are ...
    Detection and Differentiation of Multiple Viral RNAs Using Branched DNA FISH Coupled to Confocal Microscopy and Flow Cytometry
    分支DNA FISH技术联合共聚焦显微镜或流式细胞术检测和鉴别多样的病毒RNA
    作者:  Nicholas van BuurenKarla Kirkegaard, 日期: 10/20/2018, 浏览量: 3658, Q&A: 0
    Due to the exceptionally high mutation rates of RNA-dependent RNA polymerases, infectious RNA viruses generate extensive sequence diversity, leading to some of the lowest barriers to the development of antiviral drug resistance in the microbial ...
    Phenol-based Extraction of RNA from Chlamydomonas reinhardtii
    基于苯酚的莱茵衣藻RNA提取方法
    作者:  Emanuel Sanz-LuqueAmaury de Montaigu, 日期: 07/05/2018, 浏览量: 4502, Q&A: 0
    RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in Chlamydomonas. However, in some cases RNA quality and quantity may be ...
    A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria
    小RNA分离和测序方法及其在研究细菌CRISPR RNA生物合成中的应用
    Next generation high-throughput sequencing has enabled sensitive and unambiguous analysis of RNA populations in cells. Here, we describe a method for isolation and strand-specific sequencing of small RNA pools from bacteria that can be multiplexed ...
    Single Genome Sequencing of Expressed and Proviral HIV-1 Envelope Glycoprotein 120 (gp120) and nef Genes
    已表达前病毒的HIV-1包膜糖蛋白120 (gp120) 和nef基因的单基因组测序
    作者:  David J. Nolan, Susanna L. Lamers, Rebecca Rose, James J. Dollar, Marco SalemiMichael S. McGrath, 日期: 06/20/2017, 浏览量: 7100, Q&A: 0
    The current study provides detailed protocols utilized to amplify the complete HIV-1 gp120 and nef genes from single copies of expressed or integrated HIV present in fresh-frozen autopsy tissues of patients who died while on ...
    Ultradeep Pyrosequencing of Hepatitis C Virus to Define Evolutionary Phenotypes
    超深度焦磷酸测序鉴定丙型肝炎病毒的进化表型
    作者:  Brendan A. Palmer, Zoya Dimitrova, Pavel Skums, Orla Crosbie, Elizabeth Kenny-WalshLiam J. Fanning, 日期: 05/20/2017, 浏览量: 4701, Q&A: 0
    Analysis of hypervariable regions (HVR) using pyrosequencing techniques is hampered by the ability of error correction algorithms to account for the heterogeneity of the variants present. Analysis of between-sample fluctuations to virome ...
    Sequencing of Ebola Virus Genomes Using Nanopore Technology
    采用纳米孔技术对埃博拉病毒测序
    作者:  Thomas Hoenen, 日期: 11/05/2016, 浏览量: 8797, Q&A: 0
    Sequencing of virus genomes during disease outbreaks can provide valuable information for diagnostics, epidemiology, and evaluation of potential countermeasures. However, particularly in remote areas logistical and technical challenges can be ...
    Purification of Bacterial RNA from Infected Macrophages
    感染巨噬细胞的细菌RNA的纯化
    作者:  Lior Lobel, Nadejda Sigal, Anna PasechnekAnat A. Herskovits, 日期: 11/20/2015, 浏览量: 8522, Q&A: 0
    Studying the transcriptome of bacterial pathogens during infection is a very informative and effective tool for discovering genes that contribute to successful infection. However, isolating bacterial RNA from infected cells or tissues is a ...
    Quantification of Total and 2-LTR (Long terminal repeat) HIV DNA, HIV RNA and Herpesvirus DNA in PBMCs
    外周血单核细胞中DNA总量及 2-LTR (长末端重复序列) HIV DNA、HIV RNA 和 疱疹病毒 DNA 的量化
    作者:  Marta Massanella, Sara Gianella, Steven M. Lada, Douglas D. RichmanMatthew C. Strain, 日期: 06/05/2015, 浏览量: 10758, Q&A: 0
    Almost all individuals infected with human immunodeficiency virus (HIV) are also infected with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have included characterizing and measuring the latent HIV reservoir and ...