分子生物学

分类

    En masse DNA Electroporation for in vivo Transcriptional Assay in Ascidian Embryos
    海鞘胚胎中DNA电穿孔的体内转录分析
    作者:  Sébastien Darras, 日期: 09/20/2021, 浏览量: 51, Q&A: 0

    Ascidian embryos are powerful models for functional genomics, in particular, due to the ease of generating a large number of transgenic embryos by electroporation. In addition, the small size of their genome makes them an attractive model for

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    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    利用本生烟叶片基因瞬时表达或沉默研究青枯菌毒力的快速简便方法
    作者:  Wenjia YuAlberto P. Macho, 日期: 08/05/2021, 浏览量: 784, Q&A: 0

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed

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    Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts
    纤维素酶和离析酶-PEG介导的苔藓原生质体转化
    This protocol describes the generation of protoplasts from protonemal tissue of the moss Physcomitrium patens (syn. Physcomitrella patens), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) ...
    Optimize Highly Efficient Genetic Transformation Method of Spring Wheat (Triticum aestivum L.) Cultivars Using Mature Embryo
    利用成熟胚胎进行春小麦(Triticum aestivum L.)品种高效遗传转化方法的优化
    作者:  Dongjin KimMegan Hager, 日期: 11/20/2020, 浏览量: 1085, Q&A: 0
    Genetic engineering is a powerful tool to develop desired traits in crop plants to make the crops suitable for future demand and changing environments. For the genetic engineering of wheat cultivars, the development of efficient transformation and ...
    Agrobacterium-mediated Transformation of Sweet Basil (Ocimum basilicum)
    农杆菌介导的罗勒(Ocimum basilicum)转化
    作者:  Natasha NavetMiaoying Tian, 日期: 11/20/2020, 浏览量: 2312, Q&A: 1

    Sweet basil (Ocimum basilicum) is a popular herb with high economic value and is currently threatened by a severe oomycete disease. An efficient transformation method is a prerequisite for gene functional analysis to accelerate molecular breeding

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    Barley Transformation by Particle Bombardment Using Callus
    大麦愈伤组织的粒子轰击转化
    作者:  Sung-Yong Kim, 日期: 06/05/2020, 浏览量: 1637, Q&A: 0
    The delivery of desired construct into plant cells relies mainly on Agrobacterium-mediated transformation. However, Agrobacterium transformation is still very challenging for barley as only one genotype is amendable to ...
    PEG-mediated, Stable, Nuclear and Chloroplast Transformation of Cyanidioschizon merolae
    PEG介导的稳定的Cyanidioschizon merolae细胞核和叶绿体转化
    作者:  Maksymilian Zienkiewicz, Tomasz Krupnik, Anna DrożakKinga Kania, 日期: 09/05/2019, 浏览量: 2520, Q&A: 0
    The ability to achieve nuclear or chloroplast transformation in plants has been a long standing goal, especially in microalgae research. Over past years there has been only little success, but transient and stable nuclear transformation has been ...
    Development of an Efficient Transformation System for Halotolerant Yeast Debaryomyces hansenii CBS767
    耐盐汉斯德巴氏酵母菌CBS767的一种高效转化体系的建立
    作者:  Anu P. MinhasDipanwita Biswas, 日期: 09/05/2019, 浏览量: 2020, Q&A: 0
    Debaryomyces hansenii is one of the most osmotolerant and halotolerant yeasts. Further, its association with traditional cheese and meat products imparting special flavors to these products project this yeast with enormous biotechnological ...
    Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation
    枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定
    作者:  Ester SerranoBegoña Carrasco, 日期: 08/20/2019, 浏览量: 2105, Q&A: 0
    For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that ...
    Efficient Assembly of Large Multiplex CRISPR/Cas9 Guide Arrays for Maize Genome Editing
    用于玉米基因组编的大型多重CRISPR/Cas9向导阵列的组装
    作者:  Jarrett ManMadelaine E. Bartlett, 日期: 05/05/2019, 浏览量: 4295, Q&A: 0
    CRISPR/Cas9-based genome editing in maize is an effective tool, and researchers commonly wish to target multiple genes simultaneously. Transformation of maize is currently expensive and tedious, so researchers are incentivized to build vectors that ...