植物科学

分类

    Staining Maize Epidermal Leaf Peels with Toluidine Blue O
    玉米叶表皮甲苯胺蓝O染色
    作者:  Marschal A. Bellinger, Sukhmani K. SidhuCarolyn G. Rasmussen, 日期: 04/20/2019, 浏览量: 6494, Q&A: 0
    Maize is an important model organism for understanding cell patterning and development. The regular patterning of maize leaf epidermal cells has previously been characterized using the dye toluidine blue O (TBO). The polychromatic dye TBO ...
    An Image Analysis Pipeline to Quantify Emerging Cracks in Materials or Adhesion Defects in Living Tissues
    分析活体组织中出现的材料裂缝或附着力缺陷的图像分析法
    作者:  Stéphane Verger, Guillaume CeruttiOlivier Hamant, 日期: 10/05/2018, 浏览量: 4198, Q&A: 0
    Microcracks in materials reflect their mechanical properties. The quantification of the number or orientation of such cracks is thus essential in many fields, including engineering and geology. In biology, cracks in soft tissues can reflect adhesion ...
    Microscopic Observation of Subcellular GFP-tagged Protein Localization in Rice Anthers
    水稻花药中GFP标记蛋白亚细胞定位的显微镜观察
    作者:  Seijiro OnoKen-Ichi Nonomura, 日期: 08/05/2018, 浏览量: 3767, Q&A: 0
    This protocol demonstrates a simple method to determine the subcellular localization of fluorescence-tagged proteins on the vibratome sections of rice developing anthers. If a cell type-specific promoter is used to drive the tagged protein-encoding ...
    柑橘染色体涂片制片法
    Preparation of Smear Slides for Observing the Chromosomes of Citrus
    作者:  王蓉, 苗茵 , 兰红 , 陈春丽 郭文武 , 日期: 06/12/2018, 浏览量: 2453, Q&A: 0
    实验原理及目的:植物涂片法指将植物材料酶解去掉细胞壁后,经低渗液处理,用针尖敲碎后在玻片上平铺展开,火焰干燥使染色体膨胀,得到分散良好、背景干净的染色体和细胞核,又称低渗火焰干燥法。涂片制片法在植物细胞生物学、细胞遗传学 ...
    柑橘石蜡切片
    Paraffin Embedding Citrus Tissues and Sectioning
    作者:  龙健梅郭文武, 日期: 06/12/2018, 浏览量: 2698, Q&A: 0
    实验原理及目的:石蜡制片法即用石蜡作为包埋剂,将植物组织经固定、脱水、透明、浸蜡后包埋在石蜡中,然后用切片机切片。石蜡切片可用于研究植物的发育和植物器官及组织的结构,原位杂交,组织化学 (显示细胞内的某些化学成分) 等。具有易操作、切片薄 (1-12 ...
    柑橘组织细胞的激光显微切割
    Laser Microdissection of Citrus Tissue Sample
    作者:  姜楠, 贾慧慧 , 伍小萌 郭文武 , 日期: 06/12/2018, 浏览量: 2507, Q&A: 0
    实验原理及目的:通过激光显微切割系统,精细切割收集目标样品,进一步进行特定柑橘组织细胞样品的遗传信息或基因表达分析,突破了传统的取样技术限制,更为精细准确的分析特定组织细胞内分子活动。
    水稻组织石蜡切片
    Paraffin Embedding Rice Tissues and Sectioning
    作者:  程珂, 都浩欧阳亦聃, 日期: 05/30/2018, 浏览量: 6861, Q&A: 0
    实验原理:用石蜡包埋组织块制作的显微切片。经取材、固定、洗涤和脱水、透明、浸蜡、包埋、切片与粘片、脱蜡、染色、脱水、透明、封片等步骤。在显微镜下可清晰观察细胞组织的形态结构。
    实验目的:石蜡切片是研究细胞生物学的基础技术 ...
    水稻组织半薄切片法
    Observation of Rice Tissues with Semi-thin Section
    作者:  曹剑波, 程珂袁猛, 日期: 05/30/2018, 浏览量: 3808, Q&A: 0
    实验原理:使用树脂包埋样品,切成薄片的样品后能在光学显微镜下成像。
    实验目的:将树脂包埋的水稻材料切成1-2 μm的切片。
    MTT Assay for Cytotoxicity Assessment in Oryza sativa Root Tissue
    MTT法评估亚洲水稻根系组织中的细胞毒性
    作者:  Snehalata Majumdar, Titir GuhaRita Kundu, 日期: 11/20/2017, 浏览量: 7381, Q&A: 0
    Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael et al., 1987). In this ...