分子生物学

分类

    Measurement of Transgene Copy Number in Plants Using Droplet Digital PCR
    应用微滴数字PCR技术测定植物中的转基因拷贝数
    作者:  Yao-Min Cai, Quentin M. DudleyNicola J. Patron, 日期: 07/05/2021, 浏览量: 1008, Q&A: 0

    Transgenic plants are produced both to investigate gene function and to confer desirable traits into crops. Transgene copy number is known to influence expression levels, and consequently, phenotypes. Similarly, knowledge of transgene zygosity is

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    Evaluation of the Sequence Variability within the PCR Primer/Probe Target Regions of the SARS-CoV-2 Genome
    SARS-CoV-2基因组PCR引物/探针靶区序列变异性评价
    作者:  Kashif Aziz KhanPeter Cheung, 日期: 12/20/2020, 浏览量: 1827, Q&A: 0
    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; initially named 2019-nCoV) is responsible for the recent coronavirus disease (COVID-19) pandemic, and polymerase chain reaction (PCR) is the current standard method for diagnosis from ...
    Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation
    枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定
    作者:  Ester SerranoBegoña Carrasco, 日期: 08/20/2019, 浏览量: 1985, Q&A: 0
    For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that ...
    Solid Phase PCR on 3D Microstructure ArrayChip for Pathogen Detection Application
    用于病原体检测的三维显微组织列阵中固相PCR
    作者:  Krishna KantTien Anh Ngo, 日期: 08/05/2019, 浏览量: 3244, Q&A: 0
    Advanced free angle photolithography (FAPL) is presented for making 3D supercritical angle fluorescence (SAF) microstructures and transfer them on to polymeric chips using injection molding technique for low-cost microfluidic devices embedded with ...
    Quantification of Hepatitis B Virus Covalently Closed Circular DNA in Infected Cell Culture Models by Quantitative PCR
    qPCR定量分析感染细胞培养模型中乙型肝炎病毒共价闭合环状DNA
    作者:  Bingqian QuStephan Urban, 日期: 04/05/2019, 浏览量: 4440, Q&A: 0
    Persistence of the human hepatitis B virus (HBV) requires the maintenance of covalently closed circular (ccc)DNA, the episomal genome reservoir in nuclei of infected hepatocytes. cccDNA elimination is a major aim in future curative therapies ...
    PCR扩增及克隆基因
    PCR Amplification and Gene Cloning
    作者:  徐远涛徐强, 日期: 06/12/2018, 浏览量: 7288, Q&A: 0
    实验原理:PCR (polymerase chain reaction) 的原理是在模板DNA、引物和4种脱氧核糖核苷酸 (dNTP) ...
    水稻常用分子标记(SSR InDel SNP)检测
    Detection of Commonly-used Molecular Markers (SSR InDel SNP) in Rice
    作者:  孙文强, 申国境余四斌, 日期: 05/10/2018, 浏览量: 4605, Q&A: 0
    实验原理:分子标记(Molecular Markers)是以个体间核苷酸序列变异为基础的遗传标记,是DNA水平遗传多态性的直接反映。
           简单序列重复(Simple Sequence ...
    DNA甲基化检测
    Detection of DNA Methylation by Bisulfite-sequencing, Restriction Enzyme Digestion-PCR and Restriction Enzyme Digestion-Sourthern
    作者:  陈香嵩, 程赛凤, 周超, 刘潜赵毓, 日期: 05/02/2018, 浏览量: 9770, Q&A: 0
    方法一: 重亚硫酸盐测序 (Bisulfite-sequencing)

    实验原理:以重亚硫酸盐处理基因组DNA,发生甲基化的胞嘧啶不变, ...
    基于酶切方法的SNP基因分型
    Digestion-based SNP Genotyping Method
    作者:  王磊, 符德保欧阳亦聃, 日期: 04/09/2018, 浏览量: 4114, Q&A: 0
    实验原理:SNP差异是在实验过程中经常遇到的基因型多态性类型,传统方法可以通过dCAPS或者测序的方法进行鉴定,但不是每一个SNP位点都能设计出dCAPS引物,而测序成本太高,难以进行规模化鉴定。来自芹菜的核酸酶CEL ...