生物化学

分类

    Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation
    共免疫沉淀法评价哺乳动物核蛋白的自身相互作用
    作者:  Claudia Cattoglio, Iryna Pustova, Xavier Darzacq, Robert TjianAnders S. Hansen, 日期: 02/20/2020, 浏览量: 3757, Q&A: 0
    Protein-protein interactions constitute the molecular foundations of virtually all biological processes. Co-immunoprecipitation (CoIP) experiments are probably the most widely used method to probe both heterotypic and homotypic protein-protein ...
    Separation and Visualization of Low Abundant Ubiquitylated Forms
    低丰度泛素化形式蛋白的分离和可视化
    作者:  Ramona Schuster, Tânia Simões, Fabian den BraveMafalda Escobar-Henriques, 日期: 11/20/2018, 浏览量: 3194, Q&A: 0
    In this protocol we describe the separation and visualization of ubiquitylated forms of the yeast mitofusin Fzo1 by Western blot. To this aim, we express HA-tagged Fzo1 in Saccharomyces cerevisiae, break the cells to extract a ...
    Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
    蛋白质pull-down免疫共沉淀实验分析病毒DNA结合蛋白质之间的直接相互作用
    作者:  Ana Lechuga, Mónica Berjón-Otero, Margarita SalasModesto Redrejo-Rodríguez, 日期: 01/05/2018, 浏览量: 7228, Q&A: 0
    This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are ...
    Assessment of Modulation of Protein Stability Using Pulse-chase Method
    使用脉冲追踪法评估蛋白质稳定性的调节
    作者:  Mohamed Elgendy, 日期: 08/20/2017, 浏览量: 8805, Q&A: 0
    Pulse-chase technique is a method widely used to assess protein or mRNA stability. The principle of pulse-chase relies on labeling proteins or mRNA produced during a short period of time called ‘pulse’ and then following the rate of disappearance of ...
    Protein Immunoprecipitation Using Nicotiana benthamiana Transient Expression System
    采用本氏烟草瞬时表达系统进行蛋白质免疫共沉淀实验
    作者:  Fang Xu, Charles CopelandXin Li, 日期: 07/05/2015, 浏览量: 17773, Q&A: 0
    Nicotiana benthamiana (N. benthamiana) is a useful model system to transiently express protein at high level. This protocol describes in detail how to transiently express protein in N. benthamiana and how to carry out ...
    RNA-binding Protein Immunoprecipitation (RIP) to Examine AUF1 Binding to Senescence-Associated Secretory Phenotype (SASP) Factor mRNA
    RNA结合蛋白免疫共沉淀法(RIP)检验结合到衰老相关分泌表型(SASP)因子 的mRNAAUF1
    作者:  Elise AlspachSheila A. Stewart, 日期: 05/20/2015, 浏览量: 12167, Q&A: 0
    Immunoprecipitation and subsequent isolation of nucleic acids allows for the investigation of protein:nucleic acid interactions. RNA-binding protein immunoprecipitation (RIP) is used for the analysis of protein interactions with mRNA. Combining RIP ...
    Immunoprecipitation of Proteins in Caenorhabditis elegans
    免疫沉淀法检测秀丽隐杆线虫蛋白质
    作者:  Kevin K. Chan, Ashwin Seetharaman, Guillermo SelmanPeter John Roy, 日期: 04/05/2015, 浏览量: 15199, Q&A: 1
    Immunoprecipitation (IP) is a biochemical technique to precipitate a protein out of solution using an antigen that can specifically bind to that protein. IP can be performed to isolate and concentrate one particular protein from a sample of ...
    Individual-nucleotide-resolution UV Cross-linking and Immunoprecipitation (iCLIP) of UPF1
    UPF1蛋白单核苷酸分离紫外线交联和免疫沉淀(iCLIP)
    作者:  David ZündOliver Mühlemann, 日期: 04/05/2014, 浏览量: 12463, Q&A: 0
    The fate of mRNA, in particular its stability, localization and rate of translation is regulated by RNA binding proteins assembling to messenger ribonucleoprotein (mRNP) complexes. To investigate the transcriptome-wide RNA binding sites of UPF1, the ...
    UPF1 RNA Immunoprecipitation from Mini-μ Construct–expressing Cells
    Mini-μ 载体-表达细胞中的UPF1 RNA 免疫沉淀
    作者:  David ZündOliver Mühlemann, 日期: 04/05/2014, 浏览量: 9003, Q&A: 0
    UPF1, an RNA helicase and a core factor of nonsense-mediated mRNA decay (NMD), interacts with RNA independently of the sequence context. To investigate the influence of translation on the association of UPF1 with specific reporter transcripts, UPF1 ...
    Co-immunoprecipitation of Flag-TLR3 or Myc-MSR1 with HCV RNA
    Flag-TLR3 或 Myc-MSR1 与 HCV RNA的免疫共沉淀
    作者:  Daisuke Yamane, Hiromichi DansakoStanley M. Lemon, 日期: 03/05/2014, 浏览量: 8192, Q&A: 0
    Co-immunoprecipitation assay of TLR3-Flag or Myc-MSR1 with HCV RNA is used to identify direct interaction of viral RNA with host proteins that recognize viral RNA to initiate interferon (IFN) signaling, a crucial antiviral response of the host ...