生物化学

分类

    Cross-linking, Immunoprecipitation and Proteomic Analysis to Identify Interacting Proteins in Cultured Cells
    利用交联、免疫沉淀和蛋白质组学分析法鉴定培养细胞中互作的蛋白质
    作者:  Hao Wang, Meiling He, Belinda WillardQingyu Wu, 日期: 06/05/2019, 浏览量: 8753, Q&A: 0
    Extracellular expression is essential for the function of secreted and cell surface proteins. Proper intracellular trafficking depends on protein interactions in multiple subcellular compartments. Co-immunoprecipitation and the yeast two-hybrid ...
    Biochemical Analysis of Dimethyl Suberimidate-crosslinked Yeast Nucleosomes
    二甲基辛二硝酸盐交联后酵母核小体的生化分析
    作者:  Yuichi IchikawaPaul D. Kaufman, 日期: 03/20/2018, 浏览量: 5035, Q&A: 0
    Nucleosomes are the fundamental unit of eukaryotic chromosome packaging, comprised of 147 bp of DNA wrapped around two molecules of each of the core histone proteins H2A, H2B, H3, and H4. Nucleosomes are symmetrical, with one axis of symmetry ...
    An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking
    一种改进的使用时间依赖性甲醛交联测定染色质结合动力学的方法
    作者:  Elizabeth A. Hoffman, Hussain Zaidi, Savera J. Shetty, Stefan BekiranovDavid T. Auble, 日期: 02/20/2018, 浏览量: 6464, Q&A: 0
    Formaldehyde crosslinking is widely used in combination with chromatin immunoprecipitation (ChIP) to measure the locations along DNA and relative levels of transcription factor (TF)-DNA interactions in vivo. However, the measurements that ...
    Analysis of in vivo Interaction between RNA Binding Proteins and Their RNA Targets by UV Cross-linking and Immunoprecipitation (CLIP) Method
    采用紫外线交联-免疫沉淀法(CLIP)分析RNA结合蛋白与其靶标RNA的体内互作
    作者:  Pamela BielliClaudio Sette, 日期: 05/20/2017, 浏览量: 7993, Q&A: 0
    RNA metabolism is tightly controlled across different tissues and developmental stages, and its dysregulation is one of the molecular hallmarks of cancer. Through direct binding to specific sequence element(s), RNA binding proteins (RBPs) play a ...
    In vitro RNA-protein Binding Assay by UV Crosslinking
    采用紫外交联进行体外RNA-蛋白质结合试验
    作者:  Hailong ZhangMuxiang Zhou, 日期: 11/05/2012, 浏览量: 22215, Q&A: 1
    Because covalent bond can form between RNA and its binding proteins after UV irradiation, UV cross-linking is widely used to identify the specific RNA binding proteins. This protocol is described in details as follows.
    Crosslinking and Immunoprecipitation in Zebrafish
    斑马鱼胚胎中蛋白交联和免疫沉淀(最初由S. Little 撰写)
    作者:  Lili Jing, 日期: 01/20/2012, 浏览量: 13530, Q&A: 0
    Immunoprecipitation (IP) is a routine method to detect protein binding and interactions. But the weak binding between two proteins is often hard to detect during regular IP procedure. This protocol offers a crossliking and IP combination method to ...