生物化学

分类

    Intracellular IRF5 Dimerization Assay
    细胞内IRF5二聚分析
    作者:  Cherrie D. ShermanBetsy J. Barnes, 日期: 05/20/2021, 浏览量: 1937, Q&A: 0

    The intracellular interferon regulatory factor 5 (IRF5) dimerization assay is a technique designed to measure molecular interaction(s) with endogenous IRF5. Here, we present two methods that detect endogenous IRF5 homodimerization and interaction of

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    Native Co-immunoprecipitation Assay to Identify Interacting Partners of Chromatin-associated Proteins in Mammalian Cells
    天然共免疫沉淀法鉴定哺乳动物细胞中染色质相关蛋白的“伙伴蛋白质”
    作者:  Afzal Husain, Nasim A. Begum, Maki KobayashiTasuku Honjo, 日期: 12/05/2020, 浏览量: 2170, Q&A: 0

    Protein-protein interactions play key roles in nuclear processes including transcription, replication, DNA damage repair, and recombination. Co-immunoprecipitation (Co-IP) followed by western blot or mass spectrometry is an invaluable approach to

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    Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation
    共免疫沉淀法评价哺乳动物核蛋白的自身相互作用
    作者:  Claudia Cattoglio, Iryna Pustova, Xavier Darzacq, Robert TjianAnders S. Hansen, 日期: 02/20/2020, 浏览量: 3757, Q&A: 0
    Protein-protein interactions constitute the molecular foundations of virtually all biological processes. Co-immunoprecipitation (CoIP) experiments are probably the most widely used method to probe both heterotypic and homotypic protein-protein ...
    Measuring Small-molecule Inhibition of Protein Interactions in Live Cells Using FLIM-FRET
    利用FLIM-FRET进行小分子抑制蛋白相互作用的活细胞检测
    作者:  James M. Pemberton, Qian LiuDavid W. Andrews, 日期: 10/20/2019, 浏览量: 3867, Q&A: 0
    This protocol was designed to quantitatively measure small-molecule displacement of proteins in live mammalian cells using fluorescence lifetime imaging microscopy–Förster resonance energy transfer (FLIM-FRET). Tumour cell survival is often ...
    An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
    利用芽殖酵母微管相关蛋白研究微管结合和微管交联的体外显微镜检测法
    作者:  Yili Zhu, Weimin TanWei-Lih Lee, 日期: 12/05/2018, 浏览量: 4291, Q&A: 0
    In this protocol, we describe a simple microscopy-based method to assess the interaction of a microtubule-associated protein (MAP) with microtubules. The interaction between MAP and microtubules is typically assessed by a co-sedimentation assay, ...
    FRET-based Stoichiometry Measurements of Protein Complexes in vitro
    蛋白质复合物体外基于FRET化学计量学测定
    作者:  Francesca Mattiroli, Yajie GuKarolin Luger, 日期: 02/05/2018, 浏览量: 6512, Q&A: 0
    For a complete understanding of biochemical reactions, information on complex stoichiometry is essential. However, measuring stoichiometry is experimentally challenging. Our lab has developed a FRET-based assay to study protein complex stoichiometry ...
    Detection of Protein Interactions in the Cytoplasm and Periplasm of Escherichia coli by Förster Resonance Energy Transfer
    采用Förster共振能量转移法检测大肠埃希杆菌细胞质和周质中蛋白质的相互作用
    作者:  Nils Y. Meiresonne, Svetlana Alexeeva, René van der PloegTanneke den Blaauwen, 日期: 01/20/2018, 浏览量: 7650, Q&A: 0
    This protocol was developed to qualitatively and quantitatively detect protein-protein interactions in Escherichia coli by Förster Resonance Energy Transfer (FRET). The described assay allows for the previously impossible in vivo ...
    Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
    蛋白质pull-down免疫共沉淀实验分析病毒DNA结合蛋白质之间的直接相互作用
    作者:  Ana Lechuga, Mónica Berjón-Otero, Margarita SalasModesto Redrejo-Rodríguez, 日期: 01/05/2018, 浏览量: 7228, Q&A: 0
    This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are ...
    Visible Immunoprecipitation (VIP) Assay: a Simple and Versatile Method for Visual Detection of Protein-protein Interactions
    可视免疫沉淀(VIP)实验:一种简单通用的蛋白质 - 蛋白质相互作用可视化检测方法
    作者:  Yohei Katoh, Kentaro NakamuraKazuhisa Nakayama, 日期: 01/05/2018, 浏览量: 8960, Q&A: 0
    The visible immunoprecipitation (VIP) assay is a convenient alternative to conventional co-immunoprecipitation (Katoh et al., 2015). By processing lysates from cells co-expressing GFP-fusion and RFP-fusion proteins for immunoprecipitation ...
    MicroScale Thermophoresis as a Tool to Study Protein-peptide Interactions in the Context of Large Eukaryotic Protein Complexes
    微量热泳动法研究大真核生物蛋白复合物中蛋白质 - 肽相互作用
    作者:  Maximilian G. Plach, Klaus GrasserThomas Schubert, 日期: 12/05/2017, 浏览量: 18251, Q&A: 0
    Protein-peptide interactions are part of many physiological processes, for example, epigenetics where peptide regions of histone complexes are crucial for regulation of chromatin structure. Short peptides are often also used as alternatives to small ...