分子生物学

分类

    Isolation of RNA from the Murine Colonic Tissue and qRT-PCR for Inflammatory Cytokines
    从小鼠结肠组织中分离 RNA 并采用qRT -PCR 检测炎症细胞因子
    作者:  Ibrahim M. Sayed, Kaili Inouye, Soumita DasLaura Crotty Alexander, 日期: 03/20/2023, 浏览量: 290, Q&A: 0

    E-cigarette (E-cig) inhalation affects health status by modulating inflammation profiles in several organs, including the brain, lung, heart, and colon. The effect of flavored fourth-generation pod-based E-cigs (JUUL) on murine gut inflammation ...

    A Miniature Sucrose Gradient for Polysome Profiling
    用于多聚核糖体分析的微型蔗糖梯度
    作者:  Ansul LokdarshiAlbrecht G. von Arnim, 日期: 03/20/2023, 浏览量: 136, Q&A: 0

    Polysome profiling by sucrose density gradient centrifugation is commonly used to study the overall degree of translation (messenger RNA to protein synthesis). Traditionally, the method begins with synthesis of a 5–10 mL sucrose gradient onto

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    Amplification and Quantitation of Telomeric Extrachromosomal Circles
    端粒染色体外环的扩增和定量
    作者:  Nathaniel J. RobinsonWilliam P. Schiemann, 日期: 03/05/2023, 浏览量: 111, Q&A: 0

    Telomeres are structures that cap the ends of linear chromosomes and play critical roles in maintaining genome integrity and establishing the replicative lifespan of cells. In stem and cancer cells, telomeres are actively elongated by either

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    Molecular Surveillance of Malaria Using the PF AmpliSeq Custom Assay for Plasmodium falciparum Parasites from Dried Blood Spot DNA Isolates from Peru
    使用 PF AmpliSeq定制检测法对秘鲁干血斑 DNA 中的恶性疟原虫寄生虫分子进行疟疾监测

    Malaria molecular surveillance has great potential to support national malaria control programs (NMCPs), informing policy for its control and elimination. Here, we present a new three-day workflow for targeted resequencing of markers in 13

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    A Novel Method to Isolate RNase MRP Using RNA Streptavidin Aptamer Tags
    使用 RNA 链霉亲和素适体标签分离 RNase MRP 的新方法
    作者:  Violette Charteau, Merel DerksenGer J. M. Pruijn, 日期: 02/20/2023, 浏览量: 540, Q&A: 0

    Interactions between RNA-binding proteins and RNA molecules are at the center of multiple biological processes. Therefore, accurate characterization of the composition of ribonucleoprotein complexes (RNPs) is crucial. Ribonuclease (RNase) for

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    Unscheduled DNA Synthesis at Sites of Local UV-induced DNA Damage to Quantify Global Genome Nucleotide Excision Repair Activity in Human Cells
    在局部紫外线诱导的 DNA 损伤位点进行程序外DNA合成,以量化人类细胞中的全基因组核苷酸切除修复活性
    作者:  Paula J. van der Meer, Diana van den HeuvelMartijn S. Luijsterburg, 日期: 02/05/2023, 浏览量: 290, Q&A: 0

    Nucleotide excision repair (NER) removes a wide variety of structurally unrelated lesions from the genome, including UV-induced photolesions such as 6–4 pyrimidine–pyrimidone photoproducts (6-4PPs) and cyclobutane pyrimidine dimers

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    A CRISPR-based Strategy for Temporally Controlled Site-Specific Editing of RNA Modifications
    基于 CRISPR 的 RNA 修饰的时间控制位点特异性编辑策略
    作者:  Ying Xu, Yufan WangFu-Sen Liang, 日期: 02/05/2023, 浏览量: 167, Q&A: 0

    Chemical modifications on RNA play important roles in regulating its fate and various biological activities. However, the impact of RNA modifications varies depending on their locations on different transcripts and cells/tissues contexts; available

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    Isolation of Nuclei from Human Snap-frozen Liver Tissue for Single-nucleus RNA Sequencing
    从人速冻肝组织中分离细胞核用于单核 RNA 测序
    作者:  Marcus Alvarez, Jihane N. Benhammou, Shuyun Rao, Lopa Mishra, Joseph R. PisegnaPäivi Pajukanta, 日期: 02/05/2023, 浏览量: 446, Q&A: 0

    Single-nucleus RNA sequencing (snRNA-seq) provides a powerful tool for studying cell type composition in heterogenous tissues. The liver is a vital organ composed of a diverse set of cell types; thus, single-cell technologies could greatly

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    High-throughput Assessment of Mitochondrial Protein Synthesis in Mammalian Cells Using Mito-FUNCAT FACS
    使用 Mito-FUNCAT FACS 对哺乳动物细胞中线粒体蛋白合成进行高通量评估
    作者:  Hironori Saito, Tatsuya Osaki, Yoshiho IkeuchiShintaro Iwasaki, 日期: 02/05/2023, 浏览量: 216, Q&A: 0

    In addition to cytosolic protein synthesis, mitochondria also utilize another translation system that is tailored for mRNAs encoded in the mitochondrial genome. The importance of mitochondrial protein synthesis has been exemplified by the diverse

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    Preparation and Characterization of DNA-assembled GRS-DNA-CuS Nanodandelions
    DNA组装的GRS-DNA-CuS纳米蒲公英的制备和表征
    作者:  Haoze Wang, Yanna Cui, Yongming ZhangZeyu Xiao, 日期: 01/20/2023, 浏览量: 267, Q&A: 0

    In this study, we introduce a detailed protocol for the preparation of DNA-assembled GRS-DNA-copper sulfide (CuS) nanodandelion, a multifunctional theranostics nanoparticle. Using transmission electron microscope (TEM) and dynamic light

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