免疫学

分类

    Isolation of RNA from the Murine Colonic Tissue and qRT-PCR for Inflammatory Cytokines
    从小鼠结肠组织中分离 RNA 并采用qRT -PCR 检测炎症细胞因子
    作者:  Ibrahim M. Sayed, Kaili Inouye, Soumita DasLaura Crotty Alexander, 日期: 03/20/2023, 浏览量: 289, Q&A: 0

    E-cigarette (E-cig) inhalation affects health status by modulating inflammation profiles in several organs, including the brain, lung, heart, and colon. The effect of flavored fourth-generation pod-based E-cigs (JUUL) on murine gut inflammation ...

    A Tumor-admixture Model to Interrogate Immune Cell–dependent Tumorigenesis
    研究免疫细胞依赖性肿瘤发生的肿瘤混合模型
    作者:  Jordan T. Noe, Chuanlin Ding, Anne E. Geller, Beatriz E. Rendon, Jun YanRobert A. Mitchell, 日期: 03/05/2023, 浏览量: 230, Q&A: 0

    A rigorous determination of effector contributions of tumor-infiltrating immune cells is critical for identifying targetable molecular mechanisms for the development of novel cancer immunotherapies. A tumor/immune cell–admixture model is an

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    CRISPR/Cas9-based Engineering of Immunoglobulin Loci in Hybridoma Cells
    杂交瘤细胞中基于 CRISPR/Cas9 的免疫球蛋白位点工程

    Development of the hybridoma technology by Köhler and Milstein (1975) has revolutionized the immunological field by enabling routine use of monoclonal antibodies (mAbs) in research and development efforts, resulting in their successful

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    Continuous Measurement of Reactive Oxygen Species Formation in Bacteria-infected Bone Marrow–derived Macrophages Using a Fluorescence Plate Reader
    使用荧光酶标仪连续测量受细菌感染的骨髓源性巨噬细胞中活性氧的生成

    Macrophages are at the center of innate immunity and are the main target cells of the intracellular pathogen Salmonella enterica serovar Typhi. The production of reactive oxygen and nitrogen species (ROS/RNS) is the host’s early response to

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    Safety Profiling of Tumor-targeted T Cell–Bispecific Antibodies with Alveolus Lung- and Colon-on-Chip
    肿瘤靶向T细胞双特异性抗体与肺泡肺和片上结肠的安全性分析

    Traditional drug safety assessments often fail to predict complications in humans, especially when the drug targets the immune system. Rodent-based preclinical animal models are often ill-suited for predicting immunotherapy-mediated adverse

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    Sample Preparation and Integrative Data Analysis of a Droplet-based Single-Cell ATAC-sequencing Using Murine Thymic Epithelial Cells
    使用小鼠胸腺上皮细胞进行基于液滴的单细胞 ATAC 测序的样品制备和综合数据分析

    Accessible chromatin regions modulate gene expression by acting as cis-regulatory elements. Understanding the epigenetic landscape by mapping accessible regions of DNA is therefore imperative to decipher mechanisms of gene regulation under

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    Modelling Graft-Versus-Host Disease in Mice Using Human Peripheral Blood Mononuclear Cells
    使用人外周血单核细胞模拟小鼠移植物对抗宿主疾病

    Graft-versus-host disease (GvHD) is a significant complication of allogeneic hematopoietic stem cell transplantation. In order to develop new therapeutic approaches, there is a need to recapitulate GvHD effects in pre-clinical, in vivo systems, such

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    Improved Macrophage Enrichment from Mouse Skeletal Muscle
    小鼠骨骼肌巨噬细胞富集的改善

    Macrophages are a heterogeneous class of innate immune cells that offer a primary line of defense to the body by phagocytizing pathogens, digesting them, and presenting the antigens to T and B cells to initiate adaptive immunity. Through specialized

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    Human Auto-IgG Purification from High Volume Serum Sample by Protein G Affinity Purification
    用蛋白G亲和纯化法从大量血清样品中纯化人类自身 IgG
    作者:  Serena SensiAndreas Goebel, 日期: 12/05/2022, 浏览量: 321, Q&A: 0

    Immunoglobulins are proteins produced by the immune system, which bind specifically to the antigen that induced their formation and target it for destruction. Highly purified human immunoglobulins are commonly used in research laboratories for

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    Murine Double Hit Model for Neonatal Cardiopulmonary Diseases: Bronchopulmonary Dysplasia (BPD) and Pulmonary Hypertension Associated with BPD
    新生儿心肺疾病的小鼠双击模型:支气管肺发育不良(BPD)和与BPD相关的肺动脉高压
    作者:  Steven P. Garrick, Philip J. Berger, Marcel F. NoldClaudia A. Nold-Petry, 日期: 11/05/2022, 浏览量: 626, Q&A: 0

    Bronchopulmonary dysplasia (BPD) and pulmonary hypertension associated with BPD (BPD-PH) are of multifactorial origin and share common risk factors. Most murine models of BPD expose newborn pups to only one of these risk factors—more commonly

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