免疫学

分类

    Plasmodium cynomolgi Berok Growth Inhibition Assay by Thiol-reactive Probe Based Flow Cytometric Measurement
    硫醇反应探针流式细胞术检测食蟹猴疟原虫生长抑制
    作者:  Jessica Jie Ying Ong, Bruce RussellJin-Hee Han, 日期: 09/05/2021, 浏览量: 601, Q&A: 0

    The relapsing malaria species, Plasmodium vivax, is the most widely distributed and difficult-to-treat cause of human malaria. The merozoites of P. vivax preferentially invade ephemeral human CD71+ reticulocytes (nascent reticulocytes), thereby

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    Isolation and Quantification of Mouse γδT-cells in vitro and in vivo
    体外和体内小鼠γδT细胞的分离和定量
    作者:  Isha Rana, Krithika Badarinath, Ravindra K. ZirmireColin Jamora, 日期: 09/05/2021, 浏览量: 716, Q&A: 0

    The skin plays an important role in protecting the body from pathogens and chemicals in the external environment. Upon injury, a healing program is rapidly initiated and involves extensive intercellular communication to restore tissue homeostasis.

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    Microscopic Detection of ASC Inflammasomes in Bone Marrow Derived Macrophages Post Stimulation
    刺激后骨髓源性巨噬细胞中ASC炎症小体的显微检测
    作者:  Longjun Li, Rudi MaoXiaopeng Qi, 日期: 09/05/2021, 浏览量: 613, Q&A: 0

    An inflammasome is an intracellular multiprotein complex that plays important roles in host defense and inflammatory responses. Inflammasomes are typically composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD

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    Construction of a Highly Diverse mRNA Library for in vitro Selection of Monobodies
    体外选择单体的高度多样性mRNA文库的构建

    Recently, we developed transcription/translation coupled with the association of puromycin linker (TRAP) display as a quick in vitro selection method to obtain antibody-like proteins. For the in vitro selection, it is important to prepare mRNA

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    Preparation of Drosophila Larval Blood Cells for Single-cell RNA Sequencing
    果蝇幼虫血细胞单细胞RNA测序的制备
    作者:  Sudhir Gopal TattikotaNorbert Perrimon, 日期: 08/20/2021, 浏览量: 465, Q&A: 0

    Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain

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    One-step White Blood Cell Extracellular Staining Method for Flow Cytometry
    流式细胞术白细胞细胞外一步染色法
    作者:  Ines Ait Belkacem, Pénélope Bourgoin, Jean Marc Busnel, Franck GallandFabrice Malergue, 日期: 08/20/2021, 浏览量: 608, Q&A: 0

    Flow cytometry is a powerful analytical technique that is increasingly used in scientific investigations and healthcare; however, it requires time-consuming, multi-step sample procedures, which limits its use to specialized laboratories. In this

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    Isolation of Microglia and Analysis of Protein Expression by Flow Cytometry: Avoiding the Pitfall of Microglia Background Autofluorescence
    小胶质细胞的分离及蛋白表达的流式细胞仪分析:避免小胶质细胞背景荧光的陷阱
    作者:  Jeremy C. Burns, Richard M. RansohoffMichaël Mingueneau, 日期: 07/20/2021, 浏览量: 1441, Q&A: 0

    Microglia are a unique type of tissue-resident innate immune cell found within the brain, spinal cord, and retina. In the healthy nervous system, their main functions are to defend the tissue against infectious microbes, support neuronal networks

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    Production, quantification, and infection of Amazonian Phlebovirus (Bunyaviridae)
    亚马孙流域白蛉病毒属(布尼亚病毒科)的产生、定量和感染

    Phlebotomine vectors, sand flies of the order Diptera, are known to transmit Leishmania parasites as well as RNA viruses (arboviruses) to humans. The arbovirus, Icoaraci Phlebovirus (BeAN 24262 - ICOV), used in this study was isolated from Nectomys

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    A Lipidomics Approach to Measure Phosphatidic Acid Species in Subcellular Membrane Fractions Obtained from Cultured Cells
    用脂质组学方法测定培养细胞亚细胞膜组分中磷脂酸的种类
    作者:  Nawal Kassas, Laetitia Fouillen, Stéphane GasmanNicolas Vitale, 日期: 06/20/2021, 浏览量: 1128, Q&A: 0

    Over the last decade, lipids have emerged as possessing an ever-increasing number of key functions, especially in membrane trafficking. For instance, phosphatidic acid (PA) has been proposed to play a critical role in different steps along the

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    Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer
    多色流式细胞术综合分析乳腺癌小鼠模型中肿瘤免疫渗透
    作者:  Ana S. Almeida, Miriam R. FeinMikala Egeblad, 日期: 06/05/2021, 浏览量: 1665, Q&A: 0

    Flow cytometry is a popular laser-based technology that allows the phenotypic and functional characterization of individual cells in a high-throughput manner. Here, we describe a detailed procedure for preparing a single-cell suspension from mammary

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