微生物学

分类

    Isolation and Characterization of Membrane Vesicles from Lactobacillus Species
    乳酸杆菌属膜囊泡中的分离和特性研究
    作者:  Julie C. Caruana, Scott N. DeanScott A. Walper, 日期: 09/05/2021, 浏览量: 719, Q&A: 0

    Throughout their life cycle, bacteria shed portions of their outermost membrane comprised of proteins, lipids, and a diversity of other biomolecules. These biological nanoparticles have been shown to have a range of highly diverse biological

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    Plasmodium cynomolgi Berok Growth Inhibition Assay by Thiol-reactive Probe Based Flow Cytometric Measurement
    硫醇反应探针流式细胞术检测食蟹猴疟原虫生长抑制
    作者:  Jessica Jie Ying Ong, Bruce RussellJin-Hee Han, 日期: 09/05/2021, 浏览量: 601, Q&A: 0

    The relapsing malaria species, Plasmodium vivax, is the most widely distributed and difficult-to-treat cause of human malaria. The merozoites of P. vivax preferentially invade ephemeral human CD71+ reticulocytes (nascent reticulocytes), thereby

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    Microscopic Detection of ASC Inflammasomes in Bone Marrow Derived Macrophages Post Stimulation
    刺激后骨髓源性巨噬细胞中ASC炎症小体的显微检测
    作者:  Longjun Li, Rudi MaoXiaopeng Qi, 日期: 09/05/2021, 浏览量: 613, Q&A: 0

    An inflammasome is an intracellular multiprotein complex that plays important roles in host defense and inflammatory responses. Inflammasomes are typically composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD

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    Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike
    SARS-CoV-2刺突慢病毒伪型载体制备和滴定的优化方法

    The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast

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    Acid Hydrolysis for the Extraction of Archaeal Core Lipids and HPLC-MS Analysis
    酸水解法提取古生菌核心脂质及HPLC-MS分析
    作者:  Maxime Tourte, Philippe Schaeffer, Vincent GrossiPhilippe M. Oger, 日期: 08/20/2021, 浏览量: 345, Q&A: 0

    Lipid membranes are essential cellular elements as they provide cellular integrity and selective permeability under a broad range of environmental settings upon cell growth. In particular, Archaea are commonly recognized for their tolerance to

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    Monitoring Protein Splicing Using In-gel Fluorescence Immediately Following SDS-PAGE
    SDS-PAGE后立即使用凝胶内荧光监测蛋白质剪接
    作者:  Joel Weinberger IIChristopher W. Lennon, 日期: 08/20/2021, 浏览量: 1113, Q&A: 0

    Inteins garner significant interest from both basic and applied researchers due to their unique catalytic abilities. Herein, we describe a protocol for accurately monitoring protein splicing without purification using in-gel fluorescence immediately

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    Construction of a Highly Diverse mRNA Library for in vitro Selection of Monobodies
    体外选择单体的高度多样性mRNA文库的构建

    Recently, we developed transcription/translation coupled with the association of puromycin linker (TRAP) display as a quick in vitro selection method to obtain antibody-like proteins. For the in vitro selection, it is important to prepare mRNA

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    Development and Quantitation of Pseudomonas aeruginosa Biofilms after in vitro Cultivation in Flow-reactors
    流动反应器体外培养铜绿假单胞菌生物膜的研制与定量
    作者:  Yingdan Zhang, Jingru Zhao, Hang Cheng, Jing Wang, Liang YangHaihua Liang, 日期: 08/20/2021, 浏览量: 347, Q&A: 0

    Characterization of biofilm formation and metabolic activities is critical to investigating biofilm interactions with environmental factors and illustrating biofilm regulatory mechanisms. An appropriate in vitro model that mimics biofilm in vivo

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    Tomato Stem Injection for the Precise Assessment of Ralstonia solanacearum Fitness in Planta
    番茄茎杆注射法精确评价青枯菌在植物中的适应性
    作者:  Yaru Wang, Liu Xian, Gang YuAlberto P. Macho, 日期: 08/20/2021, 浏览量: 370, Q&A: 0

    Ralstonia solanacearum is a soil-borne pathogen with worldwide distribution that causes bacterial wilt disease in more than 250 plant species. R. solanacearum invades plants through the roots, reaches the vascular system, and colonizes the whole

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    Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
    植物线粒体核糖体的纯化和低温电镜分析
    作者:  Florent Waltz, Philippe GiegéYaser Hashem, 日期: 08/05/2021, 浏览量: 757, Q&A: 0

    Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production. Among

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