系统生物学

分类

    Protocol for RNA-seq Expression Analysis in Yeast
    酵母RNA-seq表达分析方法
    作者:  Stefan Bohn, 日期: 09/20/2021, 浏览量: 300, Q&A: 0

    Genome-wide sequencing of RNA (RNA-seq) has become an inexpensive tool to gain key insights into cellular and disease mechanisms. Sample preparation and sequencing are streamlined and allow the acquisition of hundreds of gene expression profiles in

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    Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays
    利用组蛋白肽阵列评估组蛋白修饰阅读蛋白的体外结合特异性
    作者:  Mark W. SooArneet L. Saltzman, 日期: 09/20/2021, 浏览量: 156, Q&A: 0

    In the field of chromatin biology, a major goal of understanding the roles of histone post-translational modifications is to identify the proteins and domains that recognize these modifications. Synthetic histone peptides containing one or more

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    Isolation of Nuclei from Mouse Dorsal Root Ganglia for Single-nucleus Genomics
    小鼠背根神经节细胞核的分离及其单核基因组学研究
    作者:  Lite Yang, Ivan Tochitsky, Clifford J. WoolfWilliam Renthal, 日期: 08/05/2021, 浏览量: 705, Q&A: 0

    Primary somatosensory neurons, whose cell bodies reside in the dorsal root ganglion (DRG) and trigeminal ganglion, are specialized to transmit sensory information from the periphery to the central nervous system. Our molecular understanding of

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    反刍动物瘤胃样品采集与保存
    Collection and Preservation of Ruminant Microbial Samples
    作者:  张小丽, 王祚, 周传社, 焦金真谭支良, 日期: 07/07/2021, 浏览量: 157, Q&A: 0
    摘要:反刍动物是农业部提倡养殖的节粮型动物,其瘤胃是自然界最完美的天然发酵罐,具备利用纤维饲料的优势。反刍动物瘤胃的研究不仅是对其营养代谢研究是必要的,对于绿色养殖更是至关重要的。因此采集瘤胃样品及保存是反刍动物相关研究人员必备的操作技术(王祚,2018; Xue ...
    厌氧微生物培养基制备与培养方法
    Preparation of Media and Culturing of Anaerobic Microbes
    作者:  赵圣国, 日期: 07/07/2021, 浏览量: 121, Q&A: 0
    摘要:厌氧微生物生活在无氧的环境中,与需氧微生物相比培养技术特殊。本文介绍了厌氧微生物培养基制备方法,具体营养成分需要根据不同微生物的种类进行选择。
    微生物超高分子量DNA提取方法
    Super-high Molecular Weight DNA Isolation
    作者:  赵圣国, 日期: 06/24/2021, 浏览量: 123, Q&A: 0
    摘要:随着三代测序的快速发展,对微生物超高分子量DNA (SHMW-DNA) 的需求越来越多,三代测序有助于大大提高微生物基因组或宏基因组组装效率。目前,常用的商业试剂盒或液体试剂提取DNA的方法,受纯化膜或液体剪切力的影响,提取的DNA片段约10-30 ...