发育生物学

分类

    Direct-TRI: High-throughput RNA-extracting Method for all Stages of Zebrafish Development
    Direct-TRI: 斑马鱼发育各阶段高通量RNA提取方法
    作者:  Kota Ujibe, Kanako Nishimura, Makoto KashimaHiromi Hirata, 日期: 09/05/2021, 浏览量: 412, Q&A: 0

    Recent popularization of next-generation sequencing enables conducting easy transcriptome analysis. Nevertheless, substantial RNA isolation work prior to RNA sequencing, as well as the high cost involved, still makes the routine use of large-scale

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    Isolation of Myofibres and Culture of Muscle Stem Cells from Adult Zebrafish
    成年斑马鱼肌纤维的分离和肌肉干细胞的培养
    作者:  Massimo Ganassi, Peter S. ZammitSimon M. Hughes, 日期: 09/05/2021, 浏览量: 1103, Q&A: 0

    Skeletal muscles generate force throughout life and require maintenance and repair to ensure efficiency. The population of resident muscle stem cells (MuSCs), termed satellite cells, dwells beneath the basal lamina of adult myofibres and contributes

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    In situ Hybridization of miRNAs in Human Embryonic Kidney and Human Pluripotent Stem Cell-derived Kidney Organoids
    在人胚肾和人多能干细胞衍生的肾类器官中微小RNA的原位杂交
    作者:  Filipa M. Lopes, Susan J. KimberIoannis Bantounas, 日期: 09/05/2021, 浏览量: 347, Q&A: 0

    MicroRNAs are small RNAs that negatively regulate gene expression and play an important role in fine-tuning molecular pathways during development. There is increasing interest in studying their function in the kidney, but the majority of studies to

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    Measuring DNA Damage Using the Alkaline Comet Assay in Cultured Cells
    培养细胞DNA损伤的碱性彗星试验
    作者:  Elena Clementi, Zuzana GarajovaEnni Markkanen, 日期: 08/20/2021, 浏览量: 893, Q&A: 0

    Maintenance of DNA integrity is of pivotal importance for cells to circumvent detrimental processes that can ultimately lead to the development of various diseases. In the face of a plethora of endogenous and exogenous DNA-damaging agents, cells

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    Preparation of Drosophila Larval Blood Cells for Single-cell RNA Sequencing
    果蝇幼虫血细胞单细胞RNA测序的制备
    作者:  Sudhir Gopal TattikotaNorbert Perrimon, 日期: 08/20/2021, 浏览量: 465, Q&A: 0

    Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain

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    Preparation of Zygotes and Embryos of the Kelp Saccharina latissima for Cell Biology Approaches
    用细胞生物学方法制备糖精海带受精卵和胚胎
    作者:  Ioannis Theodorou, Hilde-Gunn Opsahl-SortebergBénédicte Charrier, 日期: 08/20/2021, 浏览量: 164, Q&A: 0

    Embryogenesis and early development in kelps are poorly studied and understood. Cultivation protocols focus mostly either on the preparation of large volumes of vegetative gametophytes or on the production of conspicuous juvenile sporophytes as

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    Isolation and in vitro Culture of Mouse Oocytes
    小鼠卵母细胞的分离与体外培养
    作者:  Jessica Greaney, Goutham Narayanan Subramanian, Yunan YeHayden Homer, 日期: 08/05/2021, 浏览量: 852, Q&A: 0

    Females are endowed at birth with a fixed reserve of oocytes, which declines both in quantity and quality with advancing age. Understanding the molecular mechanisms regulating oocyte quality is crucial for improving the chances of pregnancy success

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    Mouse Periosteal Cell Culture, in vitro Differentiation, and in vivo Transplantation in Tibial Fractures
    小鼠骨膜细胞培养、体外分化及胫骨骨折的体内移植
    作者:  Simon Perrin, Anais Julien, Oriane Duchamp de Lageneste, Rana Abou-KhalilCéline Colnot, 日期: 08/05/2021, 浏览量: 587, Q&A: 0

    The periosteum covering the outer surface of bone contains skeletal stem/progenitor cells that can efficiently form cartilage and bone during bone repair. Several methods have been described to isolate periosteal cells based on bone scraping and/or

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    Fe-NTA Microcolumn Purification of Phosphopeptides from Immunoprecipitation (IP) Eluates for Mass Spectrometry Analysis
    Fe-NTA微柱纯化免疫沉淀(IP)洗脱物中磷酸肽的质谱分析
    作者:  Ethan J. SanfordMarcus B. Smolka, 日期: 08/05/2021, 浏览量: 489, Q&A: 0

    Protein phosphorylation is a nearly universal signaling mechanism. To date, a number of proteomics tools have been developed to analyze phosphorylation. Phosphoproteome-wide analyses using whole cell extracts suffer from incomplete coverage, often

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    A Genetically Engineered Mouse Model of Venous Anomaly and Retinal Angioma-like Vascular Malformation
    静脉畸形和视网膜血管瘤样血管畸形的基因工程小鼠模型
    作者:  Xudong Cao, Beibei Xu, Xiao Li, Taotao LiYulong He, 日期: 08/05/2021, 浏览量: 746, Q&A: 0

    Characterization of key regulators in vein development will advance our understanding of mechanisms underlying venous anomalies and provide therapeutic targets for the treatment of vascular malformations. Here, we provide a detailed protocol for the

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