生物化学

分类

    Comprehensive Identification of Translatable Circular RNAs Using Polysome Profiling
    利用多聚体谱综合鉴定可翻译环状RNA
    作者:  Yanwen Ye, Zefeng WangYun Yang, 日期: 09/20/2021, 浏览量: 514, Q&A: 0

    Circular RNAs (circRNAs), a special type of RNAs without 5’- and 3’-ends, are widely present in eukaryotes and known to function as noncoding RNAs to regulate gene expression, including as miRNA sponges. Recent studies showed that many

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    Anaerobic Expression and Purification of Holo-CCIS, an Artificial Iron-sulfur Protein
    人工铁硫蛋白 Holo-CCIS的厌氧表达与纯化
    作者:  Bhanu P. Jagilinki, Irina Paluy, Vikas NandaDror Noy, 日期: 09/20/2021, 浏览量: 429, Q&A: 0

    Iron-sulfur proteins are ubiquitous among all living organisms and are indispensable for almost all metabolic pathways ranging from photosynthesis, respiration, nitrogen, and carbon dioxide cycles. The iron-sulfur clusters primarily serve as

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    Methods for the Extraction of Heme Prosthetic Groups from Hemoproteins
    从血红素中提取血红素辅基的方法
    作者:  Kat Ellis-Guardiola, Jess SouleRobert T. Clubb, 日期: 09/20/2021, 浏览量: 609, Q&A: 0

    Hemoproteins are widely researched because they contain redox-active heme prosthetic groups (iron + protoporphyrin IX) that enable them to perform a range of vital functions, acting as enzymes, participants in electron transfer reactions, or gas

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    Cell-free Translation: Preparation and Validation of Translation-competent Extracts from Saccharomyces cerevisiae
    无细胞翻译:酿酒酵母翻译活性提取物的制备和验证
    作者:  Brandon M. Trainor, Anton A. Komar, Dimitri G. PestovNatalia Shcherbik, 日期: 09/20/2021, 浏览量: 530, Q&A: 0

    Cell-free translation is a powerful technique for in vitro protein synthesis. While cell-free translation platforms prepared from bacterial, plant, and mammalian cells are commercially available, yeast-based translation systems remain proprietary

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    Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays
    利用组蛋白肽阵列评估组蛋白修饰阅读蛋白的体外结合特异性
    作者:  Mark W. SooArneet L. Saltzman, 日期: 09/20/2021, 浏览量: 156, Q&A: 0

    In the field of chromatin biology, a major goal of understanding the roles of histone post-translational modifications is to identify the proteins and domains that recognize these modifications. Synthetic histone peptides containing one or more

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    A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin
    一种高效生产和Src激酶介导的马海兔磷酸化的共纯化方法
    作者:  Sherlene L. Brown, Yuan Ren, Daniel M. SuterSeema Mattoo, 日期: 09/20/2021, 浏览量: 232, Q&A: 0

    Cortactin is an actin-binding protein that regulates processes like cell migration, endocytosis, and tumor cell metastasis. Although cortactin is associated with actin-cytoskeletal dynamics in non-neuronal cells and cell-free systems, the exact

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    Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (KD) and the Effective Concentration (EC50) of an Interplaying Effector Molecule Using Bio-layer Interferometry
    蛋白质-蛋白质复合物的动力学分析,以确定其电离常数 (KD) 和使用生物层干涉法相互作用的效应分子的有效浓度 (EC50)
    作者:  Tim Orthwein, Luciano F. Huergo, Karl ForchhammerKhaled A. Selim, 日期: 09/05/2021, 浏览量: 978, Q&A: 0

    Biolayer interferometry (BLI) is an emerging analytical tool that allows the study of protein complexes in real time to determine protein complex kinetic parameters. This article describes a protocol to determine the KD of a protein complex using a

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    Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids
    从针叶树类囊体中分离光合巨复合体和超复合体的增溶方法
    作者:  Pushan Bag, Wolfgang P. Schröder, Stefan JanssonDomenica Farci, 日期: 09/05/2021, 浏览量: 489, Q&A: 0

    Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades. In higher plants, the process takes place in the thylakoid

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    Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike
    SARS-CoV-2刺突慢病毒伪型载体制备和滴定的优化方法

    The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast

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    One-step White Blood Cell Extracellular Staining Method for Flow Cytometry
    流式细胞术白细胞细胞外一步染色法
    作者:  Ines Ait Belkacem, Pénélope Bourgoin, Jean Marc Busnel, Franck GallandFabrice Malergue, 日期: 08/20/2021, 浏览量: 683, Q&A: 0

    Flow cytometry is a powerful analytical technique that is increasingly used in scientific investigations and healthcare; however, it requires time-consuming, multi-step sample procedures, which limits its use to specialized laboratories. In this

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