植物科学

分类

    U2.3 Precursor Small Nuclear RNA in vitro Processing Assay
    U2.3前体小核 RNA体外加工试验
    作者:  Chan Lin, Yujie Feng, Xueyan Peng, Jiaming Wu, Weili WangYunfeng Liu, 日期: 09/05/2021, 浏览量: 261, Q&A: 0

    Small nuclear RNAs (snRNAs) are vital for eukaryotic cell activities and play important roles in pre-mRNA splicing. The molecular mechanism underlying the transcription of snRNA, regulated via upstream/downstream cis-elements and relevant

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    Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids
    从针叶树类囊体中分离光合巨复合体和超复合体的增溶方法
    作者:  Pushan Bag, Wolfgang P. Schröder, Stefan JanssonDomenica Farci, 日期: 09/05/2021, 浏览量: 442, Q&A: 0

    Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades. In higher plants, the process takes place in the thylakoid

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    A Novel Method to Map Small RNAs with High Resolution
    一种新的高分辨率小RNA图谱绘制方法
    作者:  Kun Huang, Feray Demirci, Blake C. MeyersJeffrey L. Caplan, 日期: 08/20/2021, 浏览量: 545, Q&A: 0

    Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular

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    Preparation of Zygotes and Embryos of the Kelp Saccharina latissima for Cell Biology Approaches
    用细胞生物学方法制备糖精海带受精卵和胚胎
    作者:  Ioannis Theodorou, Hilde-Gunn Opsahl-SortebergBénédicte Charrier, 日期: 08/20/2021, 浏览量: 164, Q&A: 0

    Embryogenesis and early development in kelps are poorly studied and understood. Cultivation protocols focus mostly either on the preparation of large volumes of vegetative gametophytes or on the production of conspicuous juvenile sporophytes as

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    Tomato Stem Injection for the Precise Assessment of Ralstonia solanacearum Fitness in Planta
    番茄茎杆注射法精确评价青枯菌在植物中的适应性
    作者:  Yaru Wang, Liu Xian, Gang YuAlberto P. Macho, 日期: 08/20/2021, 浏览量: 370, Q&A: 0

    Ralstonia solanacearum is a soil-borne pathogen with worldwide distribution that causes bacterial wilt disease in more than 250 plant species. R. solanacearum invades plants through the roots, reaches the vascular system, and colonizes the whole

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    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    利用本生烟叶片基因瞬时表达或沉默研究青枯菌毒力的快速简便方法
    作者:  Wenjia YuAlberto P. Macho, 日期: 08/05/2021, 浏览量: 769, Q&A: 0

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed

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    Spatiotemporal Quantification of Cytosolic pH in Arabidopsis Pollen Tubes
    拟南芥花粉管胞质pH的时空定量研究
    作者:  Maria Teresa Portes, Daniel S.C. DamineliJosé A. Feijó, 日期: 07/20/2021, 浏览量: 796, Q&A: 0

    Ion-specific probes and fluorescent indicators have been key in establishing the role of ion signaling, namely calcium, protons, and anions, in plant development, providing a robust approach for monitoring spatiotemporal changes in intracellular ion

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    Measurement of Transgene Copy Number in Plants Using Droplet Digital PCR
    应用微滴数字PCR技术测定植物中的转基因拷贝数
    作者:  Yao-Min Cai, Quentin M. DudleyNicola J. Patron, 日期: 07/05/2021, 浏览量: 1284, Q&A: 0

    Transgenic plants are produced both to investigate gene function and to confer desirable traits into crops. Transgene copy number is known to influence expression levels, and consequently, phenotypes. Similarly, knowledge of transgene zygosity is

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    Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS
    气质联用技术分析水稻组织中微量可溶性糖含量
    作者:  Li-he Xu, Li-ying Xiao, Xue-qiong Xiao, Yan-nong XiaoGao-feng Wang, 日期: 07/05/2021, 浏览量: 1470, Q&A: 0

    Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies

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    Micrografting in Arabidopsis Using a Silicone Chip
    利用硅芯片进行拟南芥微嫁接
    作者:  Hiroki Tsutsui, Yaichi KawakatsuMichitaka Notaguchi, 日期: 06/20/2021, 浏览量: 1575, Q&A: 1

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including

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