神经科学

分类

    Measurement of LRRK2 Kinase Activity by Proximity Ligation Assay
    通过邻近连接测定法测量 LRRK2 激酶活性
    作者:  Matthew T. Keeney, Eric K. Hoffman, J. Timothy GreenamyreRoberto Di Maio, 日期: 09/05/2021, 浏览量: 407, Q&A: 0

    Missense mutations in leucine rich-repeat kinase 2 (LRRK2) cause forms of familial Parkinson’s disease and have been linked to ‘idiopathic’ Parkinson’s disease. Assessment of LRRK2 kinase activity has been very challenging

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    Clearing of the Mouse Brain for Optical Imaging Using CUBIC
    用CUBIC技术清除小鼠大脑的光学成像
    作者:  Lianxiang Luo, Xiaoyu Chen, Ruijian Wu, Han WangHui Luo, 日期: 08/20/2021, 浏览量: 202, Q&A: 0

    Here, we describe a simple and efficient tissue clearing method, CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis), which was first introduced in 2014 (Susaki et al., 2014). In the present study, the hemispheres of

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    Unraveling the Physicochemical Determinants of Protein Liquid-liquid Phase Separation by Nanoscale Infrared Vibrational Spectroscopy
    利用纳米尺度红外振动光谱揭示蛋白质液-液相分离的理化决定因素
    作者:  Francesco S. Ruggeri, Alyssa M. Miller, Michele VendruscoloTuomas P. J. Knowles, 日期: 08/20/2021, 浏览量: 847, Q&A: 0

    The phenomenon of reversible liquid-liquid phase separation of proteins underlies the formation of membraneless organelles, which are crucial for cellular processes such as signalling and transport. In addition, it is also of great interest to

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    3D-printed Recoverable Microdrive and Base Plate System for Rodent Electrophysiology
    用于啮齿动物电生理学的3D打印可恢复微驱动器和基板系统

    Extracellular recordings in freely moving animals allow the monitoring of brain activity from populations of neurons at single-spike temporal resolution. While state-of-the-art electrophysiological recording devices have been developed in recent

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    小鼠脑切片原位杂交
    In-situ Hybridization for Mouse Brain Sections
    作者:  应玥, 蔡予琦, 吴锦云何苗, 日期: 08/10/2021, 浏览量: 139, Q&A: 0
    摘要:原位杂交 (In-situ hybridization, ISH) ...
    Cell-attached and Whole-cell Patch-clamp Recordings of Dopamine Neurons in the Substantia Nigra Pars Compacta of Mouse Brain Slices
    小鼠脑片黑质致密部多巴胺神经元的细胞贴附和全细胞膜片钳记录
    作者:  Stefano Cattaneo, Maria Regoni, Jenny SassoneStefano Taverna, 日期: 08/05/2021, 浏览量: 746, Q&A: 0

    The Substantia Nigra pars compacta (SNc) is a midbrain dopaminergic nucleus that plays a key role in modulating motor and cognitive functions. It is crucially involved in several disorders, particularly Parkinson’s disease, which is

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    A Simple Spatial-independent Associative and Reversal Learning Task in Mice
    小鼠的一个简单的空间独立的联想和逆转学习任务
    作者:  Fulvio Magara, Benjamin Boury-JamotHanna Hörnberg, 日期: 08/05/2021, 浏览量: 532, Q&A: 0

    The ability to adapt one's behavior in response to changing circumstances, or cognitive flexibility, is often altered in neuropsychiatric and neurodevelopmental conditions. In rodents, cognitive flexibility is frequently assessed using associative

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    CRISPR-mediated Labeling of Cells in Chick Embryos Based on Selectively Expressed Genes
    基于选择性表达基因的CRISPR介导的鸡胚胎细胞标记
    作者:  Masahito YamagataJoshua R. Sanes, 日期: 08/05/2021, 浏览量: 1192, Q&A: 0

    The abilities to mark and manipulate specific cell types are essential for an increasing number of functional, structural, molecular, and developmental analyses in model organisms. In a few species, this can be accomplished by germline transgenesis;

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    Optical Clearing and 3D Analysis Optimized for Mouse and Human Pancreata
    小鼠和人胰腺的光学清除和三维分析优化

    The pancreas is a heavily innervated organ, but pancreatic innervation can be challenging to comprehensively assess using conventional histological methods. However, recent advances in whole-mount tissue clearing and 3D rendering techniques have

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    Isolation of Nuclei from Mouse Dorsal Root Ganglia for Single-nucleus Genomics
    小鼠背根神经节细胞核的分离及其单核基因组学研究
    作者:  Lite Yang, Ivan Tochitsky, Clifford J. WoolfWilliam Renthal, 日期: 08/05/2021, 浏览量: 663, Q&A: 0

    Primary somatosensory neurons, whose cell bodies reside in the dorsal root ganglion (DRG) and trigeminal ganglion, are specialized to transmit sensory information from the periphery to the central nervous system. Our molecular understanding of

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