干细胞

分类

    In situ Hybridization of miRNAs in Human Embryonic Kidney and Human Pluripotent Stem Cell-derived Kidney Organoids
    在人胚肾和人多能干细胞衍生的肾类器官中微小RNA的原位杂交
    作者:  Filipa M. Lopes, Susan J. KimberIoannis Bantounas, 日期: 09/05/2021, 浏览量: 347, Q&A: 0

    MicroRNAs are small RNAs that negatively regulate gene expression and play an important role in fine-tuning molecular pathways during development. There is increasing interest in studying their function in the kidney, but the majority of studies to

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    Isolation of Myofibres and Culture of Muscle Stem Cells from Adult Zebrafish
    成年斑马鱼肌纤维的分离和肌肉干细胞的培养
    作者:  Massimo Ganassi, Peter S. ZammitSimon M. Hughes, 日期: 09/05/2021, 浏览量: 1103, Q&A: 0

    Skeletal muscles generate force throughout life and require maintenance and repair to ensure efficiency. The population of resident muscle stem cells (MuSCs), termed satellite cells, dwells beneath the basal lamina of adult myofibres and contributes

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    Neutral Comet Assay to Detect and Quantitate DNA Double-Strand Breaks in Hematopoietic Stem Cells
    检测和定量造血干细胞DNA双链断裂的中性彗星试验
    作者:  Irene Mariam Roy, Pon Sowbhagya NadarSatish Khurana, 日期: 08/20/2021, 浏览量: 461, Q&A: 0

    In vertebrates, hematopoietic stem cells (HSCs) regulate the supply of blood cells throughout the lifetime and help to maintain homeostasis. Due to their long lifespan, genetic integrity is paramount for these cells, and accordingly, a number of

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    Isolation of Single Cells from Mouse Periodontal Ligament
    小鼠牙周膜单细胞的分离
    作者:  Jing ZhaoPaul Sharpe, 日期: 08/20/2021, 浏览量: 838, Q&A: 0

    The periodontal ligament (PDL) is an essential tissue connecting teeth and bone. It is a complex tissue specifically designed to absorb the forces of mastication; analysis of its multiple cell populations is important to understand its function and

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    Mouse Periosteal Cell Culture, in vitro Differentiation, and in vivo Transplantation in Tibial Fractures
    小鼠骨膜细胞培养、体外分化及胫骨骨折的体内移植
    作者:  Simon Perrin, Anais Julien, Oriane Duchamp de Lageneste, Rana Abou-KhalilCéline Colnot, 日期: 08/05/2021, 浏览量: 587, Q&A: 0

    The periosteum covering the outer surface of bone contains skeletal stem/progenitor cells that can efficiently form cartilage and bone during bone repair. Several methods have been described to isolate periosteal cells based on bone scraping and/or

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    A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines
    小胶质细胞的分离及蛋白表达的流式细胞仪分析:避免小胶质细胞背景荧光的陷阱
    作者:  Kohei NishimuraTatsuo Fukagawa, 日期: 07/20/2021, 浏览量: 686, Q&A: 0

    Inducing loss of function of a target protein using methods such as gene knockout is a powerful and useful strategy for analyzing protein function in cells. In recent years, the CRISPR/Cas-9-based gene knockout technology has been widely used across

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    CRISPR/Cas9-mediated Precise SNP Editing in Human iPSC Lines
    CRISPR/Cas9介导的人iPSC细胞系SNP精确编辑
    作者:  Hanwen ZhangSiwei Zhang, 日期: 06/20/2021, 浏览量: 1457, Q&A: 0

    Human induced pluripotent stem cells (hiPSCs) have been extensively used in the fields of developmental biology and disease modeling. CRISPR/Cas9 gene editing in iPSC lines often has a low frequency, which hampers its application in precise allele

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    Generation and Maintenance of Homogeneous Human Midbrain Organoids
    同种人类中脑类器官的生成与维持
    作者:  Henrik Renner, Martha Grabos, Hans R. SchölerJan M. Bruder, 日期: 06/05/2021, 浏览量: 2050, Q&A: 0

    Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a

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    Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis
    小鼠多能干细胞衍生干细胞样结构的生成:植入术后胚胎发生的体外模型

    Post-implantation mammalian embryogenesis involves profound molecular, cellular, and morphogenetic changes. The study of these highly dynamic processes is complicated by the limited accessibility of in utero development. In recent years, several

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    Muscle Cryoinjury and Quantification of Regenerating Myofibers in Mice
    小鼠肌肉低温损伤及再生肌纤维的定量研究
    作者:  Yori Endo, Mehran KarvarIndranil Sinha, 日期: 06/05/2021, 浏览量: 1383, Q&A: 0

    Cryoinjury, or injury due to freezing, is a method of creating reproducible, local injuries in skeletal muscle. This method allows studying the regenerative response following muscle injuries in vivo, thus enabling the evaluation of local and

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