分子生物学

分类

    High-throughput Assessment of Mitochondrial Protein Synthesis in Mammalian Cells Using Mito-FUNCAT FACS
    使用 Mito-FUNCAT FACS 对哺乳动物细胞中线粒体蛋白合成进行高通量评估
    作者:  Hironori Saito, Tatsuya Osaki, Yoshiho IkeuchiShintaro Iwasaki, 日期: 02/05/2023, 浏览量: 79, Q&A: 0

    In addition to cytosolic protein synthesis, mitochondria also utilize another translation system that is tailored for mRNAs encoded in the mitochondrial genome. The importance of mitochondrial protein synthesis has been exemplified by the diverse

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    Preparation of Caenorhabditis elegans for Scoring of Muscle-derived Exophers
    用于肌肉源性外显体评估的秀丽隐杆线虫的制备
    作者:  Katarzyna Banasiak, Michał TurekWojciech Pokrzywa, 日期: 01/05/2023, 浏览量: 290, Q&A: 0

    Utilizingresources available from the mother's body to guarantee healthy offspring growth is the fundamental reproductive strategy. Recently, we showed that a class of the largest extracellular vesicles known as exophers, which are responsible

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    BONCAT-based Profiling of Nascent Small and Alternative Open Reading Frame-encoded Proteins
    基于 BONCAT 的新生小和替代开放阅读框架编码蛋白质的分析
    作者:  Xiongwen Cao, Yanran Chen, Alexandra KhitunSarah A. Slavoff, 日期: 01/05/2023, 浏览量: 255, Q&A: 0

    RIBO-seq and proteogenomics have revealed that mammalian genomes harbor thousands of unannotated small and alternative open reading frames (smORFs, <100 amino acids, and alt-ORFs, >100 amino acids, respectively). Several dozen mammalian

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    Fluorescence Screens for Identifying Central Nervous System–Acting Drug–Biosensor Pairs for Subcellular and Supracellular Pharmacokinetics
    用于识别亚细胞和超细胞药代动力学的中枢神经系统作用的药物-生物传感器配对的荧光筛选

    Subcellular pharmacokinetic measurements have informed the study of central nervous system (CNS)–acting drug mechanisms. Recent investigations have been enhanced by the use of genetically encoded fluorescent biosensors for drugs of interest at the

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    Proteome Integral Solubility Alteration (PISA) Assay in Mammalian Cells for Deep, High-Confidence, and High-Throughput Target Deconvolution
    在哺乳动物细胞中进行蛋白质组积分溶解度改变(PISA)测定,用于深度、高置信度和高通量目标解构
    作者:  Xuepei Zhang, Olga Lytovchenko, Susanna L. Lundström, Roman A. ZubarevMassimiliano Gaetani, 日期: 11/20/2022, 浏览量: 895, Q&A: 0

    Chemical proteomics focuses on the drug–target–phenotype relationship for target deconvolution and elucidation of the mechanism of action—key and bottleneck in drug development and repurposing. Majorly due to the limits of using

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    Fluorescent Binding Protein Sensors for Detection and Quantification of Biochemicals, Metabolites, and Natural Products
    用于检测和量化生化物质、代谢物和天然产物的荧光结合蛋白传感器
    作者:  Salete M. NewtonPhillip E. Klebba, 日期: 11/20/2022, 浏览量: 972, Q&A: 0

    Membrane transporters and soluble binding proteins recognize particular nutrients, metabolites, vitamins, or ligands. By modifying genetically engineered single cysteine residues near the active sites of such proteins with extrinsic maleimide

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    Single-molecule Force Spectroscopy on Biomembrane Force Probe to Characterize Force-dependent Bond Lifetimes of Receptor–ligand Interactions on Living Cells
    生物膜力探针的单分子力谱表征活细胞上受体-配体相互作用的力依赖性键的寿命
    作者:  Tongtong Zhang, Chenyi An, Wei HuWei Chen, 日期: 10/20/2022, 浏览量: 933, Q&A: 0

    The transmembrane receptor–ligand interactions play a vital role in the physiological and pathological processes of living cells, such as immune cell activation, neural synapse formation, or viral invasion into host cells. Mounting evidence

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    A Novel Imaging Technique for The On-site Assessment of Renal Biopsy Specimens
    一种用于肾活检标本现场评估的新型成像技术
    作者:  Tomoaki Takata, Hajime Isomoto, Takuji IyamaKentaro Yamada, 日期: 09/20/2022, 浏览量: 544, Q&A: 0

    When performing renal biopsy, it is necessary to identify the cortex, where glomeruli are exclusively distributed, to ensure the quality of the specimen for histological diagnosis. However, conventional methods using a stereomicroscope or magnifying

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    In-Cell Western Protocol for Semi-High-Throughput Screening of Single Clones
    单克隆半高通量筛选的In-Cell Western实验方案
    作者:  Arpita S. Pal, Alejandra M. AgredoAndrea L. Kasinski, 日期: 08/20/2022, 浏览量: 1564, Q&A: 0

    The in-cell western (ICW) is an immunocytochemical technique that has been used to screen for effects of siRNAs, drugs, and small molecule inhibitors. The reduced time and number of cells required to follow this protocol illustrates its

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