植物科学

分类

    Quantitative Analysis of Redox Pool (NAD+, NADH Content) in Plant Samples Under Aluminum Stress
    铝胁迫下植物样品氧化还原池(NAD+, NADH含量)的定量分析
    作者:  Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki KoyamaSanjib Kumar Panda, 日期: 06/20/2022, 浏览量: 1431, Q&A: 0

    Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial

    ...
    Investigation of Transposon DNA Methylation and Copy Number Variation in Plants Using Southern Hybridisation
    利用Southern杂交技术研究植物中转座子DNA甲基化和拷贝数变异的情况
    作者:  Vivek Hari Sundar G.P. V. Shivaprasad, 日期: 06/05/2022, 浏览量: 919, Q&A: 0

    Plant genomes are pronouncedly enriched in repeat elements such as transposons. These repeats are epigenetically regulated by DNA methylation. Whole genome high-depth sequencing after bisulfite treatment remains an expensive and laborious method to

    ...
    In vitro Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions
    评估蛋白质-磷脂相互作用的特异性和亲和力的体外测定

    Protein–lipid interactions play important roles in many biological processes, including metabolism, signaling, and transport; however, computational and structural analyses often fail to predict such interactions, and determining which lipids

    ...
    Apoplastic Expression of CARD1-ecto Domain in Nicotiana benthamiana and Purification from the Apoplastic Fluids
    本氏烟草中 CARD1-ecto 结构域的质外体表达及质外体液的纯化
    作者:  Nobuaki Ishihama, Anuphon Laohavisit, Kaori TakizawaKen Shirasu, 日期: 04/20/2022, 浏览量: 1364, Q&A: 0

    The protein expression and purification process is an essential initial step for biochemical analysis of a protein of interest. Traditionally, heterologous protein expression systems (such as E. coli, yeast, insect cells, and cell-free) are employed

    ...
    In vitro Auto- and Substrate-Ubiquitination Assays
    体外自体和底物泛素化测定
    作者:  Hye Lin Park, Han Yong LeeGyeong Mee Yoon, 日期: 04/05/2022, 浏览量: 1012, Q&A: 1

    The precise regulation of the homeostasis of the cellular proteome is critical for the appropriate growth and development of plants. It also allows the plants to respond to various environmental stresses, by modulating their biochemical and

    ...
    Activity-based Protein Profiling of Serine Hydrolase Superfamily Enzymes
    丝氨酸水解酶超家族酶的基于活性的蛋白质分析
    作者:  Achintya Kumar Dolui, Mahadev LathaPanneerselvam Vijayaraj, 日期: 03/20/2022, 浏览量: 905, Q&A: 0

    Activity-based protein profiling (ABPP) is a chemoproteomics platform to assess the functional state of enzymes in complex biological systems. Over the two decades, ABPP has emerged from a gel-based to gel-free platform, for in-depth proteome

    ...
    A Quick Method to Quantify Iron in Arabidopsis Seedlings
    一种快速定量拟南芥幼苗中铁的方法
    作者:  Chandan Kumar Gautam, Huei-Hsuan TsaiWolfgang Schmidt, 日期: 03/05/2022, 浏览量: 1724, Q&A: 0

    Iron (Fe) is an indispensable micronutrient for plant growth and development. Since both deficiency, as well as a surplus of Fe, can be detrimental to plant health, plants need to constantly tune uptake rates to maintain an optimum level of Fe.

    ...
    Measuring Endogenous GA and IAA
    测量内源性 GA 和 IAA
    作者:  Yunping Chen, Zhiwu DanShaoqing Li, 日期: 02/20/2022, 浏览量: 1471, Q&A: 0

    Plant hormones regulate many physiological processes that largely influence growth, differentiation, and development. Contents of phytohormones were analyzed using a liquid chromatography-electrospray ionization-tandem mass spectrometry

    ...
    An in vitro Coupled Assay for PEPC with Control of Bicarbonate Concentration
    一种控制碳酸氢盐浓度的磷酸烯醇式丙酮酸羧化酶的体外偶联分析
    作者:  Nicholas R. Moody, Chatawal PhansopalJames D. Reid, 日期: 12/20/2021, 浏览量: 1183, Q&A: 0

    Phosphoenolpyruvate carboxylase (PEPC) catalyzes a critical step in carbon metabolism in plants and bacteria, the irreversible reaction between bicarbonate and phosphoenolpyruvate to produce the C4 compound oxaloacetate. This enzyme is particularly

    ...
    Preference Test of Plutella xylostella Larvae upon DMNT Treatment
    小菜蛾幼虫对DMNT处理的偏好试验
    作者:  Chen Chen, Zhen Tao, Chuanhong Wang, Tengyue Wang, Hongyi Chen, Taoshan JiangPeijin Li, 日期: 11/05/2021, 浏览量: 1149, Q&A: 0

    We describe a method to test the preference of insects in response to (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). We use a device that includes a horizontal glass tube, two grooves (with activated carbon), air flow, rubber stoppers/tubes, transparent

    ...