植物科学

分类

    Profiling of Single-cell-type-specific MicroRNAs in Arabidopsis Roots by Immunoprecipitation of Root Cell-layer-specific GFP-AGO1
    通过根细胞层特异性 GFP-AGO1 的免疫沉淀分析拟南芥根中单细胞类型特异性 MicroRNAs
    作者:  Lusheng Fan, Bin GaoXuemei Chen, 日期: 12/20/2022, 浏览量: 326, Q&A: 0

    MicroRNAs (miRNA) are small (21–24 nt) non-coding RNAs involved in many biological processes in both plants and animals. The biogenesis of plant miRNAs starts with the transcription of MIRNA (MIR) genes by RNA polymerase II; then, the

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    Faster Bacterial Gene Cloning Using the Brick into the Gateway (BiG) Protocol
    使用Brick into the Gateway (BiG) 实验方法快速克隆细菌基因

    Cloning systems like Gateway and Golden Gate/Braid are known because of their efficiency and accuracy. While the main drawback of Gateway is the expensive cost of the enzymes used in its two-step (LR and BP) reaction, Golden Gate requires

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    Measurement of Transgenes Copy Number in Wheat Plants Using Droplet Digital PCR
    利用微滴数字 PCR 测量小麦植株中的转基因拷贝数
    作者:  Peng Liu, Shuang Liu, Jiajia Lei, Jianping ChenJian Yang, 日期: 12/05/2022, 浏览量: 346, Q&A: 0

    Genetic transformation is a powerful method for the investigation of gene function and improvement of crop plants. The transgenes copy number in the transgenic line is involved in gene expression level and phenotypes. Additionally, identification of

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    Chromosome Scaffolding of Diploid Genomes Using ALLHiC
    使用ALLHiC构建二倍体基因组的染色体支架
    作者:  Yi-Bin WangXing-Tan Zhang, 日期: 09/20/2022, 浏览量: 167, Q&A: 0

    High-throughput chromosome conformation capture (Hi-C) technology has become an economical and robust tool for generating a chromosome-scale assembly. However, high-quality chromosome scaffoldings are limited by the number of short and chimeric

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    The Canu Genome Assembly Pipeline Using Nanopore Long Reads
    使用 Nanopore Long Reads 的 Canu 基因组组装流程
    作者:  Guifang LinSanzhen Liu, 日期: 09/05/2022, 浏览量: 354, Q&A: 0

    Long sequencing reads have greatly improved assemblies of genomes with all sizes. The current Oxford Nanopore technology can regularly produce reads longer than 20 kb with less than 10% sequencing errors. To use long reads that contain relatively

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    Contigs Scaffolding with Hi-C for Plant Genomes
    用Hi-C构建植物基因组Contigs Scaffolding
    作者:  Hong An, Qing Xiao, Zhibo Jia, J. Chris PiresBin Yi, 日期: 08/05/2022, 浏览量: 526, Q&A: 0

    Hi-C is a chromosome conformation capture method originally developed to detect genome-wide chromatin interactions. Nowadays, it is widely applied in scaffolding de novo assembled contigs into chromosome-scale genome sequences. Multiple open-source

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    Transformation, Normalization, and Batch Effect Removal
    转换、归一化和批量效应去除
    作者:  Lei Yu, Han Qu, Qiong Jia, Xuesong WangZhenyu Jia, 日期: 07/20/2022, 浏览量: 721, Q&A: 0

    In bulk RNA-seq analysis, normalization and batch effect removal are two procedures necessary to scale the read counts and reduce technical errors. Many differential expression analysis tools require a raw count matrix as input and embed the

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    Quality Control and Preprocessing of Sequencing Reads
    测序读数的质量控制和预处理
    作者:  Zhiqiang Hao, Xiaojuan LiangGuanglin Li, 日期: 07/05/2022, 浏览量: 592, Q&A: 0

    Quality control and preprocessing of sequences are essential before analyzing high-throughput sequence data. After raw read data is generated from high-through sequencing platforms, quality control and preprocessing of sequencing reads should

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    Differential Expression Analysis: Simple Pair, Interaction, Time-series
    微分表达式分析:简单对、交互、时间序列
    作者:  Han Qu, Meng Qu, Shibo Wang, Lei Yu, Qiong Jia, Xuesong WangZhenyu Jia, 日期: 07/05/2022, 浏览量: 764, Q&A: 0

    Identifying differentially expressed (DE) genes across specific conditions is vital in understanding phenotypic variation. The fast-growing RNA sequencing (RNA-seq) provides much information that efficiently quantifies gene expression. Methods and

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    Maize Genome Assembly with PacBio Reads
    利用PacBio Reads组装玉米基因组
    作者:  Ying HuMarcio F. R. Resende Jr., 日期: 07/05/2022, 浏览量: 385, Q&A: 0

    Assembly of high-quality genomes is critical for the characterization of structural variations (SV), for the development of a high-resolution map of polymorphisms, and to serve as the foundation for gene annotation. In recent years, the advent

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