细胞生物学

分类

    Unscheduled DNA Synthesis at Sites of Local UV-induced DNA Damage to Quantify Global Genome Nucleotide Excision Repair Activity in Human Cells
    在局部紫外线诱导的 DNA 损伤位点进行程序外DNA合成,以量化人类细胞中的全基因组核苷酸切除修复活性
    作者:  Paula J. van der Meer, Diana van den HeuvelMartijn S. Luijsterburg, 日期: 02/05/2023, 浏览量: 144, Q&A: 0

    Nucleotide excision repair (NER) removes a wide variety of structurally unrelated lesions from the genome, including UV-induced photolesions such as 6–4 pyrimidine–pyrimidone photoproducts (6-4PPs) and cyclobutane pyrimidine dimers

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    Dual-Color Live Imaging of Adult Muscle Stem Cells in the Embryonic Tissues of Drosophila melanogaster
    果蝇胚胎组织成体肌肉干细胞的活体成像
    作者:  Monika Zmojdzian, Binoj Dhanarajan, Krzysztof JaglaRajaguru Aradhya, 日期: 02/05/2023, 浏览量: 33, Q&A: 0

    Adult muscle stem cells (MuSCs) show remarkable capability in repairing injured tissues. Studying MuSCs in suitable model organisms, which show strong homology with vertebrate counterparts, helps in dissecting the mechanisms regulating their

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    In vivo Assessment of Lysosomal Stress in the Drosophila Brain Using Confocal Fluorescence Microscopy
    利用共聚焦荧光显微镜对果蝇大脑溶酶体压力的体内评估
    作者:  Felipe Martelli, 日期: 01/20/2023, 浏览量: 215, Q&A: 0

    Lysosomes play a central role in signaling, nutrient sensing, response to stress, and the degradation and recycling of cellular content. Defects in lysosomal digestive enzymes or structural components can impair lysosomal function with dire

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    Targeting Ultrastructural Events at the Graft Interface of Arabidopsis thaliana by A Correlative Light Electron Microscopy Approach
    用相关光电子显微镜的方法确定拟南芥嫁接界面的超微结构单元
    作者:  Clément Chambaud, Sarah J. Cookson, Nathalie Ollat, Amélie BernardLysiane Brocard, 日期: 01/20/2023, 浏览量: 556, Q&A: 0

    Combining two different plants together through grafting is one of the oldest horticultural techniques. In order to survive, both partners must communicate via the formation of de novo connections between the scion and the rootstock. Despite the

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    Myonecrosis Induction by Intramuscular Injection of CTX
    肌内注射 CTX 诱导肌肉坏死

    Skeletal muscle, one of the most abundant tissue in the body, is a highly regenerative tissue. Indeed, compared to other tissues that are not able to regenerate after injury, skeletal muscle can fully regenerate upon mechanically, chemically,

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    Preparation of Caenorhabditis elegans for Scoring of Muscle-derived Exophers
    用于肌肉源性外显体评估的秀丽隐杆线虫的制备
    作者:  Katarzyna Banasiak, Michał TurekWojciech Pokrzywa, 日期: 01/05/2023, 浏览量: 290, Q&A: 0

    Utilizingresources available from the mother's body to guarantee healthy offspring growth is the fundamental reproductive strategy. Recently, we showed that a class of the largest extracellular vesicles known as exophers, which are responsible

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    Monitoring Mitochondrial Protein Import Using Mitochondrial Targeting Sequence (MTS)-eGFP
    使用线粒体靶向序列 (MTS)-eGFP 监测线粒体蛋白导入
    作者:  Jonas B. Michaelis, Süleyman Bozkurt, Jasmin A. SchäferChristian Münch, 日期: 12/20/2022, 浏览量: 417, Q&A: 0

    Mitochondria are cellular organelles essential for the function and survival of eukaryotic cells. Nearly all mitochondrial proteins are nuclear-encoded and require mitochondrial import upon their synthesis in the cytosol. Various approaches

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    Focused Ion Beam Milling and Cryo-electron Tomography Methods to Study the Structure of the Primary Cell Wall in Allium cepa
    用聚焦离子束铣削和低温电子断层扫描术方法研究洋葱原代细胞壁的结构
    作者:  William J. Nicolas, Grant J. JensenElliot M. Meyerowitz, 日期: 12/05/2022, 浏览量: 490, Q&A: 0

    Cryo-electron tomography (cryo-ET) is a formidable technique to observe the inner workings of vitrified cells at a nanometric resolution in near-native conditions and in three-dimensions. One consequent drawback of this technique is the sample

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    Fluorescence Time-lapse Imaging of Entosis Using Tetramethylrhodamine Methyl Ester Staining
    使用四甲基罗丹明甲酯染色对内吞死亡进行荧光延时成像
    作者:  Emir Bozkurt, Heiko DüssmannJochen H. M. Prehn, 日期: 12/05/2022, 浏览量: 326, Q&A: 0

    Entosis is a process where a living cell launches an invasion into another living cell’s cytoplasm. These inner cells can survive inside outer cells for a long period of time, can undergo cell division, or can be released. However, the fate

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