细胞生物学

分类

    A Mouse Infection Model with a Wildtype Salmonella enterica Serovar Typhimurium Strain for the Analysis of Inflammatory Innate Immune Cells
    用于分析炎性先天免疫细胞的野生型肠沙门氏菌鼠伤寒血清型小鼠感染模型
    作者:  Christa Pfeifhofer-Obermair, Natascha Brigo, Piotr TymoszukGünter Weiss, 日期: 04/05/2022, 浏览量: 1019, Q&A: 0

    Salmonella enterica serovar Typhimurium (S. Typhimurium) is a Gram-negative, facultative intracellular bacterium, which causes gastrointestinal disorders in humans, and systemic, typhoid fever-like infections in mice. Our current knowledge regarding

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    Single-cell Damagenome Profiling by Linear Copying and Splitting based Whole Genome Amplification (LCS-WGA)
    基于线性复制和分裂的全基因组扩增法(LCS-WGA)分析单细胞损伤组
    作者:  Yichi Niu, Qiangyuan ZhuChenghang Zong, 日期: 03/20/2022, 浏览量: 1313, Q&A: 0

    Spontaneous DNA damage frequently occurs on the human genome, and it could alter gene expression by inducing mutagenesis or epigenetic changes. Therefore, it is highly desired to profile DNA damage distribution on the human genome and identify the

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    Single Cell Analysis and Sorting of Aspergillus fumigatus by Flow Cytometry
    用流式细胞仪对烟曲霉进行单细胞分析和分选
    作者:  Gareth HowellRobert-Jan Bleichrodt, 日期: 04/20/2021, 浏览量: 3129, Q&A: 0

    Experimental results in fungal biology research are usually obtained as average measurements across whole populations of cells, whilst ignoring what is happening at the single cell level. Microscopy has allowed us to study single-cell behavior, but

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    海洋细菌活性和膜完整性的流式检测
    Assessment of the Bacterial Cell Viability and Membrane Integrity in Marine Bacteria by Flow Cytometry
    作者:  钟春莲, 卢余盛, 贾力陈建明, 日期: 11/20/2019, 浏览量: 2587, Q&A: 0
    摘要:5-氰基-2,3-二甲苯基四氮唑 (5-cyano-2,3-ditolyl tetrazolium chloride,CTC) 是一种氧化还原染料,本身不带荧光。CTC可以通过活细菌细胞膜,被活细菌还原后,产生红色荧光物质。碘化丙啶 (Propidium ...
    海洋微藻中脂质体含量的流式检测
    Flow Cytometric Determination of Lipid Content in Marine Microalgae
    作者:  钟春莲, 卢余盛, 贾力陈建明, 日期: 11/20/2019, 浏览量: 2849, Q&A: 0
    摘要:微藻脂质体,俗称微藻油,其中富油微藻可作为一种新型的生物柴油来源,对其有效的开发和利用可缓解全球石油供缺问题。本文采用尼罗红 (NR) 染料对微藻中油脂染色,通过流式细胞仪检测荧光强度,从而快速、准确的测定微藻中脂质体含量,筛选富含脂质体的微藻,即富油微藻。
    基于不同类型海洋微藻所含色素成分差异的流式分群分析
    Marine Microalgae Pigment Analysis by Flow Cytometry
    作者:  钟春莲, 卢余盛, 贾力陈建明, 日期: 11/20/2019, 浏览量: 2809, Q&A: 0
    摘要:藻类植物的主要特征为细胞内含有大量的色素,因其含有色素种类和数量的不同,不同的藻类呈现不同的颜色,因此色素以其特征显著用于微藻群落的分析。本文通过流式细胞仪检测海洋微藻中含有的特征色素成分差异可实现对大批样品中微藻种类鉴定和数量分析的初步研究。
    骨骼肌肌肉干细胞流式分选
    Isolation of Skeletal Muscle Stem Cells by FACS
    作者:  韩莉莉胡苹, 日期: 11/20/2019, 浏览量: 4008, Q&A: 0
    摘要:为了提高分离效率,采用两步法进行肌肉干细胞的分离和分选。首先分离小鼠骨骼肌,并将肌肉组织消化为单细胞悬液。然后利用流式分选的方法,使用杂细胞标签CD45、CD31和Sca ...