分子生物学

分类

    BONCAT-based Profiling of Nascent Small and Alternative Open Reading Frame-encoded Proteins
    基于 BONCAT 的新生小和替代开放阅读框架编码蛋白质的分析
    作者:  Xiongwen Cao, Yanran Chen, Alexandra KhitunSarah A. Slavoff, 日期: 01/05/2023, 浏览量: 228, Q&A: 0

    RIBO-seq and proteogenomics have revealed that mammalian genomes harbor thousands of unannotated small and alternative open reading frames (smORFs, <100 amino acids, and alt-ORFs, >100 amino acids, respectively). Several dozen mammalian

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    Proteome Integral Solubility Alteration (PISA) Assay in Mammalian Cells for Deep, High-Confidence, and High-Throughput Target Deconvolution
    在哺乳动物细胞中进行蛋白质组积分溶解度改变(PISA)测定,用于深度、高置信度和高通量目标解构
    作者:  Xuepei Zhang, Olga Lytovchenko, Susanna L. Lundström, Roman A. ZubarevMassimiliano Gaetani, 日期: 11/20/2022, 浏览量: 886, Q&A: 0

    Chemical proteomics focuses on the drug–target–phenotype relationship for target deconvolution and elucidation of the mechanism of action—key and bottleneck in drug development and repurposing. Majorly due to the limits of using

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    In-Cell Western Protocol for Semi-High-Throughput Screening of Single Clones
    单克隆半高通量筛选的In-Cell Western实验方案
    作者:  Arpita S. Pal, Alejandra M. AgredoAndrea L. Kasinski, 日期: 08/20/2022, 浏览量: 1554, Q&A: 0

    The in-cell western (ICW) is an immunocytochemical technique that has been used to screen for effects of siRNAs, drugs, and small molecule inhibitors. The reduced time and number of cells required to follow this protocol illustrates its

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    A High-throughput Automated ELISA Assay for Detection of IgG Antibodies to the SARS-CoV-2 Spike Protein
    一种高通量自动ELISA检测SARS-CoV-2刺突蛋白IgG抗体的方法

    The SARS-CoV-2 pandemic and vaccination campaign has illustrated the need for high throughput serological assays to quantitatively measure antibody levels. Here, we present a protocol for a high-throughput colorimetric ELISA assay to detect IgG

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    Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike
    SARS-CoV-2刺突慢病毒伪型载体制备和滴定的优化方法

    The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast

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    Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease
    用于研究人类疾病中蛋白质N-糖基化失调的N-糖肽丰度和N-糖基化位点的差异分析
    作者:  Qi Zhang, Cheng Ma, Lian LiLih-Shen Chin, 日期: 06/20/2021, 浏览量: 2461, Q&A: 0

    Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass

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    In vitro Measurement of Membrane Attack Complex in RPE Cells
    视网膜色素上皮细胞膜攻击复合物的体外测定
    作者:  Kelly MulfaulSarah L. Doyle, 日期: 02/20/2021, 浏览量: 2954, Q&A: 0

    Initiation of the complement system results in the formation of a multiprotein pore termed the membrane attack complex (MAC, C5b-C9). MAC pores accumulate on a cell surface and can result in cell lysis. The retinal pigment epithelium (RPE) is a

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    Assembly and Imaging Set up of PIE-Scope
    PIE-Scope的组装和成像设置
    作者:  Sergey Gorelick, David A. Dierickx, Genevieve Buckley, James C. WhisstockAlex de Marco, 日期: 10/05/2020, 浏览量: 3632, Q&A: 0
    Cryo-Electron Tomography (cryo-ET) is a method that enables resolving the structure of macromolecular complexes directly in the cellular environment. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both ...
    Quantification of the Surface Expression of G Protein-coupled Receptors Using Intact Live-cell Radioligand Binding Assays
    利用完整活细胞放射配体结合分析定量G蛋白偶联受体的表面表达
    作者:  Xin XuGuangyu Wu, 日期: 09/20/2020, 浏览量: 3284, Q&A: 0
    G protein-coupled receptors (GPCRs) are the most structurally diverse family of signaling proteins and regulate a variety of cell function. For most GPCRs, the cell surface is their functional destination where they are able to respond a wide range ...
    A Workflow for Ultra-rapid Analysis of Histone Post-translational Modifications with Direct-injection Mass Spectrometry
    使用直接进样质谱对组蛋白翻译后修饰进行超快速分析的工作流程
    作者:  Natarajan V. Bhanu, Simone SidoliBenjamin A Garcia, 日期: 09/20/2020, 浏览量: 3959, Q&A: 0
    Chromatin modifications, like histone post translational modifications (PTMs), are critical for tuning gene expression and many other aspects of cell phenotype. Liquid chromatography coupled to mass spectrometry (LC-MS) has become the most suitable ...